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Mallotus furetianus homopolysaccharide as well as preparation method and application thereof

A technology of camellia kucha and polysaccharides, applied in the field of natural polymers, to achieve the effects of high-efficiency DPPH free radicals, anti-oxidation and inhibition of α-glucosidase activity, and scavenging DPPH free radicals

Inactive Publication Date: 2019-09-13
SOUTH CHINA UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on polysaccharides of camellia kucha mainly focuses on the extraction process of crude polysaccharides, and there are few reports on the relationship between its structure and biological activity.

Method used

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  • Mallotus furetianus homopolysaccharide as well as preparation method and application thereof
  • Mallotus furetianus homopolysaccharide as well as preparation method and application thereof
  • Mallotus furetianus homopolysaccharide as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment example 1

[0041] Implementation case 1: Preparation of Camellia polysaccharide MFP-2A

[0042] (1) Polysaccharide extraction:

[0043] Take 1000g of the washed and dried camellia, pulverize it with a pulverizer, and pass it through a 60-mesh sieve. Add 6L of absolute ethanol and reflux for two hours in a water bath at 70℃. Dry in a blast oven, add 20L of water, extract for 4 hours at 80°C in a water bath, centrifuge, filter, concentrate to 2.5L, and slowly add four times the volume of 10L absolute ethanol at 500r / min. Let it stand for 12 hours at -4°C, centrifuge, collect the precipitate, and then wash it with absolute ethanol and acetone three times in sequence. The precipitate is freeze-dried to obtain 30.4 g crude polysaccharide of camellia.

[0044] (2) In addition to protein:

[0045] Dissolve 20 g of the above-prepared camellia crude polysaccharide in 300 mL of water, stir, centrifuge, add Sevag reagent to the supernatant, shake, stand still, centrifuge, take the supernatant, then add Se...

Embodiment example 2

[0060] Implementation case 2: Antioxidant activity experiment of camellia polysaccharide MFP-2A

[0061] The anti-oxidant activity of polysaccharides was evaluated by DPPH, ABTS method and superoxide radical experiment, as follows:

[0062] Scavenging DPPH free radicals: Put 150μL of MFP-2A solution (0.2, 0.4, 0.6, 0.8, 1.0, 1.2, 1.4, 1.6, 1.8 and 2.0mg / mL) and 150μL of DPPH solution (60μmol / L) in a 96-well plate respectively Mix and react for 30 minutes in the dark, test the absorbance of the mixture at 517nm, and use ascorbic acid (Vc) as a positive control. Figure 5 A.

[0063] Scavenging ABTS free radicals: Mix 2 mL of ABTS (7 mmol / L) solution and 2 mL of potassium persulfate aqueous solution (2.45 mmol / L), and react for 12 hours in the dark to prepare cationic ABTS free radicals. The prepared cationic ABTS radical solution was diluted with ethanol to an absorbance value of 0.7±0.05 at 734nm. Add 20μL of polysaccharide solution (0.2, 0.4, 0.6, 0.8, 1.0, 1.2, 1.4, 1.6, 1.8 and ...

Embodiment example 3

[0066] Implementation case 3: Camellia polysaccharide MFP-2A inhibits α-glycosidase activity experiment

[0067] Add 20μL of polysaccharide solution (0.2, 0.4, 0.6, 0.8, 1.0, 1.2, 1.4, 1.6, 1.8 and 2.0mg / mL) and 40μL of α-glycosidase solution (0.2U / mL) to a 96-well plate, mix well, Incubate for 10 min at 37℃, then add 20.0μL pNPG (10mM), mix well, react at 37℃ for 30min, and finally add 100μL Na 2 CO 3 (0.2M) solution to stop the reaction, test the absorbance of the mixture at 405nm, take acarbose as a positive control, see the result Image 6 .

[0068] The results showed that Camellia polysaccharide MFP-2A has inhibitory α-glycosidase activity in the concentration range of 0.2-2.0 mg / mL, and its inhibitory activity increases with the increase of concentration. At a concentration of 2.0 mg / mL, the inhibition rate of MFP-2A was 68.42±1.21%. It shows that Camellia polysaccharide has a good inhibitory effect on α-glycosidase activity.

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Abstract

The invention belongs to the technical field of natural polymers and discloses a mallotus furetianus homopolysaccharide with antioxidant activity and alpha-glucosidase inhibition activity as well as apreparation method and application thereof. The mallotus furetianus homopolysaccharide disclosed by the invention is named as MFP-2A, and the structural formula of the mallotus furetianus homopolysaccharide is as shown in the specification. The mallotus furetianus homopolysaccharide MFP-2A in the invention is an acid polysaccharide, simultaneously contains six glucosidic bonds of -4,6)-Galp-(1-,-3,6)-Manp-(1-,-5)-Araf-(1-,T-Araf-(1-,-4)-GalpA-(1- and -4)-Glc-(1-, particularly contains alpha-(1,4) glucosidic bonds, has high-efficiency abilities of scavenging DPPH (diphenyl-1-picrylhydrazyl) free radicals, ABTS (azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt) free radicals and superoxide radicals, also has properties of resisting oxidation and inhibiting alpha-glucosidaseactivity, can serve as a natural antioxidant, and is applied to preparation of hypoglycemic drugs or health foods.

Description

Technical field [0001] The invention belongs to the technical field of natural polymers, and particularly relates to a homogenous camellia polysaccharide with both antioxidant and α-glycosidase inhibitory activities, and a preparation method and application thereof. Background technique [0002] Type 2 diabetes is a non-insulin dependent disease. Due to its insulin secretion defect and insulin resistance, it will cause symptoms of postprandial hyperglycemia. Postprandial hyperglycemia is one of the main inducing factors for the complications of type 2 diabetes. Inhibiting glycosidase hydrolysis is an effective way to relieve postprandial hyperglycemia. α-Glucosidase is a glycoside hydrolase, which hydrolyzes α-D-glucose residues connected to the terminal non-reducing -1→4, thereby releasing glucose into the blood, leading to postprandial hyperglycemia. Therefore, inhibition of α-glucosidase can reduce the digestion of carbohydrates and slow down the postprandial blood sugar lev...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P39/06A61P3/10A23L33/125
CPCA23V2002/00A61K31/715A23L33/125A61P3/10A61P39/06C08B37/0003A23V2250/51
Inventor 李琳陈君诚李冰张霞郑青松霍达梁毅
Owner SOUTH CHINA UNIV OF TECH
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