Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method capable of simultaneously detecting various anticholinergic drugs

An anticholinergic drug and detection method technology, applied in the field of medical detection, can solve problems such as long detection time, and achieve the effects of reducing detection cost, less sample volume, and accurate detection results

Inactive Publication Date: 2019-09-13
GUILIN UNIVERSITY OF TECHNOLOGY
View PDF5 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method can achieve the separation and analysis of scopolamine and hyoscyamine, the detection time is as long as 50 minutes and only two kinds of tropane alkaloids can be separated.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method capable of simultaneously detecting various anticholinergic drugs
  • Method capable of simultaneously detecting various anticholinergic drugs
  • Method capable of simultaneously detecting various anticholinergic drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048]Embodiment 1 of the present invention is: a method capable of simultaneously detecting multiple anticholinergic drugs, and the detected sample is a methanol solution containing atropine, scopolamine methyl bromide, anisodamine and scopolamine butyl bromide. Wherein, the structural formulas of atropine, scopolamine methyl bromide, anisodamine and scopolamine butyl bromide are shown in A, B, C and D in the following formula successively:

[0049]

[0050] The instrument information used is as follows: capillary electrophoresis electrospray ionization mass spectrometry is Agilent 3D CE and Agilent 1100 Series single quadrupole mass spectrometer (Agilent Technologies Inc.). Capillary electrophoresis and mass spectrometry were coupled through the vertical sheath electrospray ion source interface of Agilent G1607A (Agilent Technologies Inc.). The sheath fluid is delivered by an Agilent 1100 series constant flow liquid chromatography pump (Agilent Technologies Inc., 1:100 s...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
lengthaaaaaaaaaa
Login to View More

Abstract

The invention discloses a method capable of simultaneously detecting various anticholinergic drugs. The anticholinergic drugs at least comprise atropine, anisodamine, scopolamine methobromide and scopolamine butylbromide; the detection method adopts CE-ESI-MS (Capillary Electrophoresis-Electrospray mass spectrometry), in the detection process, the separation voltage, the buffer solution, the sheath fluid type and the sheath fluid flow velocity parameter are set as follows: the separation voltage is 10-21 kV; the buffer solution is an ammonium acetate buffer solution with a pH value of 9.4, thesheath fluid type is an isopropanol-aqueous solution added with acetic acid, and the sheath fluid flow velocity is 2.0-4.0 [mu]L / min. According to the scheme, high-efficiency and rapid separation andanalysis can be realized by adopting a CE-MS coupling technique, thereby reducing the consumption of an organic solvent; compared with an HPLC-MS (High Performance Liquid Chromatography-Mass Spectrometry) technique in the prior art, the method has the advantages of being small in sample amount and the like; and the scheme in the invention is combined with high-efficiency separation of capillary electrophoresis and an accurate qualitative function of mass spectra, so that the detection result is more accurate.

Description

technical field [0001] The invention relates to the technical field of medical detection, in particular to a method capable of simultaneously detecting multiple anticholinergic drugs. Background technique [0002] Tropane alkaloids are a class of anticholinergic drugs with M receptor blocking effect. There are more than 200 known natural tropane alkaloids, which mainly exist in plants such as Solanaceae, Convolvulaceae and Mangroves. The tropane alkaloids with pharmacological activity mainly include atropine, anisodamine and scopolamine derivatives, etc. Among them, atropine has a wide range of pharmacological effects such as stimulating the central nervous system, dilating mydriasis, relieving spasm and inhibiting glandular secretion, and has many side effects. Clinically, it is mainly used for various visceral colic (such as stomach pain, intestinal colic, renal colic) and mydriasis, which can relieve acute organophosphate poisoning. The pharmacological effect of anisodam...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/447G01N27/62
CPCG01N27/44756G01N27/62
Inventor 张庆于晓章林钰涓杨丽雷生煜楚云鹏黄丽丽
Owner GUILIN UNIVERSITY OF TECHNOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com