Preparing method of plant cell enzymolysis extracts for treating avian influenza
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A plant cell and extract technology, applied in the field of preparation of plant cell enzymolysis extract, can solve the problems of insignificant virus inhibition, drug side effect drug resistance, slow absorption, etc., avoid side effects and drug resistance, improve The effect of survival
Pending Publication Date: 2019-09-17
任辛
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[0003] At present, if poultry are found to be suffering from highly pathogenic avian influenza, a comprehensive measure of strict isolation and blockade and culling of the whole group is basically adopted. Although this can suppress the spread of the virus, it will undoubtedly cause huge losses to the poultry breeding industry. Economic losses
There are also Chinese patent medicines for the prevention and treatment of bird flu on the market now, but they are absorbed slowly after taking them, and have no obvious inhibitory effect on the virus. Disadvantages of drug resistance
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Embodiment 1
[0022] A method for preparing a plant cell enzymolysis extract for treating avian influenza in the present invention comprises the following steps:
[0023] Step S01: Weigh fresh garlic and bitter gourd, wash them, mix and crush them into 10 meshes, add an equal amount of mature vinegar with a mass concentration of 30%, and edible grain wine with a mass concentration of 50%, and place them in an enzymatic dissolver, wherein the mature vinegar 1. The temperature of edible grain wine is 25°C, and garlic, bitter gourd, mature vinegar, and edible grain wine are all added in equal amounts.
[0024] Step S02, add amylase into the enzymolysis dissolver, the feeding ratio of amylase is 10%, stir at high speed for 20 minutes, stir once a day, add chitooligosaccharide cell fusion enzyme after continuous stirring for 5 days, and chitooligosaccharide cell fusion The feeding ratio of the enzyme is 1.5%, adding sodium carboxymethyl cellulose with a feeding ratio of 6%, and standing for 30 d...
Embodiment 2
[0027] A method for preparing a plant cell enzymolysis extract for treating avian influenza in the present invention comprises the following steps:
[0028] Step S01, weigh fresh garlic and bitter gourd, wash them, mix and crush them into 20 meshes, add an equal amount of mature vinegar with a mass concentration of 30%, and edible grain wine with a mass concentration of 50%, and place them in an enzymatic solution. 1. The temperature of edible grain wine is 30°C, and garlic, balsam pear, mature vinegar, and edible grain wine are all added in equal amounts.
[0029] Step S02, add amylase into the enzymolysis dissolver, the feeding ratio of amylase is 10%, stir at high speed for 20 minutes, stir once a day, add chitooligosaccharide cell fusion enzyme after continuous stirring for 5 days, and chitooligosaccharide cell fusion The feeding ratio of the enzyme is 1.5%, adding sodium carboxymethyl cellulose with a feeding ratio of 6%, and standing for 30 days until the garlic cells an...
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Abstract
The invention discloses a preparing method of plant cell enzymolysis extracts for treating avian influenza. The method includes the following steps of weighing fresh garlic and bitter gourd to be cleaned, mixing and smashing the garlic and bitter gourd until the granularity of 10-20 mesh is reached, adding mature vinegar and edible grain liquor into an enzymolysis container, adding starch protease into the enzymolysis container to be rotated and stirred for 20 minutes at a high speed once every day, adding a chitosan oligosaccharide cell fusion enzyme after stirring is continuously conducted for 5 days, mixing a growth promoting agent in cooperation for standing for 30 days, and conducting clarifying, filtering, separating and extracting on fused cell metabolites at a normal temperature. According to the preparing method of the plant cell enzymolysis extracts for treating avian influenza, the garlic and bitter gourd are pure natural medicine and food homologous plant objects, substances extracted after cell fusion enzymolysis keep the existing characteristics of the garlic and bitter gourd of immunity, inflammation eliminating, bacterial killing, virus resisting and the like and also have the medicine property of killing avian influenza viruses, the side effects and medicine tolerance caused by the use of a large amount of antibiotics are avoided, and the survival rate of poultry is increased.
Description
technical field [0001] The invention relates to the technical field of medicines, in particular to a preparation method of a plant cell enzymolysis extract for treating avian influenza. Background technique [0002] Avian influenza, the full name of avian influenza, is an acute infectious disease caused by avian influenza virus. It is generally only transmitted among birds. In rare cases, it can infect pigs, and in rare cases, it can cross the species barrier and infect humans. Poultry infected with avian influenza virus will mainly have systemic or respiratory diseases. Poultry such as chickens, turkeys, ducks, and quails, as well as wild birds such as wild birds, waterfowl, and seabirds, can be infected. The incidence ranges from acute septicemia to asymptomatic Toxic and so on are extremely diverse. [0003] At present, if poultry are found to be suffering from highly pathogenic avian influenza, a comprehensive measure of strict isolation and blockade and culling of the ...
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