Biocontrol strain TMN-1, and preparation method and application of biocontrol microbial inoculum

Abstract

The invention relates to the technical field of plant protection, and in particular, relates to a biocontrol strain TMN-1, and a preparation method and an application of a biocontrol microbial inoculum. The biocontrol strain TMN-1 is a hypocrea fungus; preparation of the biocontrol microbial inoculum containing the biocontrol strain TMN-1 comprises the steps: taking rhizosphere soil of healthy tobacco plants in an ill land where bacterial wilt occurs in a tobacco planting area, screening the biocontrol strain TMN-1 on a rose-bengal selective culture medium by a dilution coating plate method, activating the screened biocontrol strain TMN-1 on a PDA culture medium, carrying out shake cultivation on the obtained pure biocontrol strain TMN-1 in a PD culture solution at the speed of 180 rpm and at the temperature of 28 DEG C for 5 d, centrifuging at the speed of 7000 rpm for 8-10 min, and adjusting the spore concentration by sterile water to 1*10<8> cfu / mL. The biocontrol microbial inoculum can promote the healthy growth of tobacco, improve the structure of soil microbial community, have good control effect on tobacco bacterial wilt and reduce the harm of tobacco bacterial wilt when being applied in tobacco planting.

Description

technical field [0001] The invention relates to the technical field of plant protection, in particular to a preparation method and application of a biocontrol strain TMN-1 and a biocontrol agent. Background technique [0002] Tobacco bacterial wilt, also known as tobacco blast, is a soil-borne bacterial disease caused by Ralstia solanacearum, and it is also a typical vascular disease. The death of the whole plant is one of the devastating diseases of tobacco, which seriously affects the yield of tobacco leaves. The devastating harm of R. solanacearum to tobacco was first reported in Indonesia in 1864. Bacteria wilt occurs widely in tobacco planting areas in my country at present, and flue-cured tobacco planting areas such as Henan, Sichuan, Chongqing, Guizhou, and Yunnan occur every year. With the increase of continuous cropping years, continuous cropping obstacles cannot be broken, resulting in increased soil acidification and soil fertility. decline, soil health loses its...

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Problems solved by technology

Bacteria wilt occurs widely in tobacco planting areas in my country at present, and flue-cured tobacco planting areas such as Henan, Sichuan, Chongqing, Guizhou, and Yunnan occur every year. With the increase of continuous cropping years, continuous cropping obstacles cannot be broken, resulting in increased soil acidification and soil fertility. decline, soil health loses its original balance, crops cannot grow healthily, their ability to resist diseases decreases, and soil pathogens continue to accumulate, leading to the outbreak of tobacco bacterial wilt

[0003] So far, there is no effective method or agent for the prevention and treatment of tobacco bacterial wilt. Chemical control is the main method of prevention and control, but it will bring serious environmental problems; cultivating resistant varieties is a better solution, but my country has a large area and the bacterial wilt Disease races are more differentiated and mutate rapidly, and resistant varieties will quickly lose disease resistance, which is not a long-term solution

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