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Process for coating composite microecologiccal preparation by using solution commixing method

A composite microecology and solution blending technology, applied in the field of immobilization, can solve the problems of inconvenient transportation of bacterial liquid and short storage period, and achieve the effects of easy application and promotion, low toxicity and short production cycle.

Active Publication Date: 2019-09-27
DONGGUAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with the bacterial liquid product, it is easy to carry, easy to store, and saves space, which solves the problems of inconvenient transportation and short storage period of the bacterial liquid, and can be promoted in the aquaculture industry

Method used

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  • Process for coating composite microecologiccal preparation by using solution commixing method
  • Process for coating composite microecologiccal preparation by using solution commixing method
  • Process for coating composite microecologiccal preparation by using solution commixing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Step a. Prepare the seed solution of the composite microecological preparation: culture yeast (ACCC20165) with YPD medium at 30℃ for 24h; LB broth medium at 30℃ cultivate Bacillus (CICC20037) for 24h, LB broth medium at 30℃ cultivate photosynthetic bacteria ( CCREMSDMCC150016) 48h; Lactobacillus (ACCC11016) cultured in MRS medium at 30℃ for 48h;

[0025] The formulas of YPD medium, broth medium and MRS medium are as follows:

[0026] LB broth medium: tryptone 10g / L, yeast extract 5g / L, NaCl 10g / L, distilled water 1L, pH6.8-7.0;

[0027] YPD medium: glucose 20g / L, peptone 20g / L, yeast extract 10g / L, distilled water 1L;

[0028] MRS medium: glucose 20g / L, peptone 10g / L, beef extract 10g / L, yeast extract 5g / L, anhydrous sodium acetate 5g / L, K 2 HPO 4 2g / L, triammonium citrate 2g / L, MgSO 4 0.2g / L, MnSO 4 0.05g / L, 1L distilled water, pH 6.2-6.4;

[0029] Step b. Prepare a bacterial suspension of the composite microecological preparation: inoculate the four bacteria, Bacillus, Lact...

Embodiment 2

[0040] Step a. Prepare the seed solution of the composite microecological preparation: culture yeast (ACCC20165) with YPD medium at 30℃ for 24h; LB broth medium at 30℃ cultivate Bacillus (CICC20037) for 24h, LB broth medium at 30℃ cultivate photosynthetic bacteria ( CCREMSDMCC150016) 48h; Lactobacillus (ACCC11016) cultured in MRS medium at 30℃ for 48h;

[0041] The formulas of YPD medium, broth medium and MRS medium are as follows:

[0042] LB broth medium: tryptone 10g / L, yeast extract 5g / L, NaCl 10g / L, distilled water 1L, pH6.8-7.0;

[0043] YPD medium: glucose 20g / L, peptone 20g / L, yeast extract 10g / L, distilled water 1L;

[0044] MRS medium: glucose 20g / L, peptone 10g / L, beef extract 10g / L, yeast extract 5g / L, anhydrous sodium acetate 5g / L, K 2 HPO 4 2g / L, triammonium citrate 2g / L, MgSO 4 0.2g / L, MnSO 4 0.05g / L, 1L distilled water, pH 6.2-6.4;

[0045] Step b. Prepare the bacterial suspension of the composite microecological preparation: combine Bacillus CICC20037, lactic acid b...

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Abstract

The invention relates to an immobilization technique for preparing a PVA-SA-zeolite powder copolymer microsphere coating from sodium alginate (SA) and polyvinyl alcohol (PVA) as raw materials, calcium chloride and boric acid as crosslinking agents together with a small amount of modified zeolite powder as a carrier and a composite microecologiccal preparation bacterial liquid. The production process provided by the invention is simple and easy to operate, efficient, safe and degradable in production raw material and easy to popularize and use. The process is applied to holothurian culture, the water purification effect of a composite microecologiccal preparation can be improved, the water purification time can be prolonged, the composite microecologiccal preparation can be easily preserved and transported when being compared with a bacterial liquid product mode, and the problems that the microecologiccal preparation is short in effect taking cycle and inconvenient to transport and preserve in the application process can be solved.

Description

Technical field [0001] The invention relates to immobilization technology, in particular to a method for preparing copolymer gel microspheres by immobilization of a composite probiotic preparation. Background technique [0002] At present, the compound microecological preparation has been used in aquaculture, mainly in the sea cucumber aquaculture industry. It can be directly sprayed into the aquaculture water body, and can also be used as a feed additive to purify the water quality of the aquaculture water body, promote the growth of the sea cucumber, and improve the intestine The role of digestive enzyme activity in the tract. However, in practical applications, there are problems such as inconvenient transportation, short shelf life, and unstable effects. Summary of the invention [0003] In order to solve the above problems, the present invention adopts a solution blending method using sodium alginate (SA) and polyvinyl alcohol (PVA) as raw materials, calcium chloride and bor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N11/10C12N11/08C12N11/04C12R1/865C12R1/125C12R1/25C12R1/01
CPCC12N1/14C12N11/04C12N11/08C12N11/10
Inventor 王际辉肖珊陆璐
Owner DONGGUAN UNIV OF TECH
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