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Process of embedding composite microecological preparations by solution blending method

A technology of composite microecology and solution blending, applied in the field of immobilization, can solve the problems of short storage period and inconvenient transportation of bacterial liquid, and achieve the effects of non-toxicity, low toxicity, easy application and promotion, and wide sources

Active Publication Date: 2021-03-23
DONGGUAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with the bacterial liquid product, it is easy to carry, easy to store, and saves space, which solves the problems of inconvenient transportation and short storage period of the bacterial liquid, and can be promoted in the aquaculture industry

Method used

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  • Process of embedding composite microecological preparations by solution blending method
  • Process of embedding composite microecological preparations by solution blending method
  • Process of embedding composite microecological preparations by solution blending method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Step a, preparation of compound microecological preparation seed liquid: use YPD medium to cultivate yeast (ACCC20165) at 30°C for 24h; LB broth medium to cultivate bacillus (CICC20037) at 30°C for 24h, and LB broth medium to cultivate photosynthetic bacteria at 30°C ( CCREMSDMCC150016) 48h; MRS culture medium 30 ℃ lactic acid bacteria (ACCC11016) 48h;

[0025] Wherein YPD medium, broth medium and MRS medium formulations are as follows:

[0026] LB broth medium: tryptone 10g / L, yeast extract powder 5g / L, NaCl 10g / L, distilled water 1L, pH6.8-7.0;

[0027] YPD medium: glucose 20g / L, peptone 20g / L, yeast extract 10g / L, distilled water 1L;

[0028] MRS medium: glucose 20g / L, peptone 10g / L, beef extract 10g / L, yeast extract 5g / L, anhydrous sodium acetate 5g / L, K 2 HPO 4 2g / L, triammonium citrate 2g / L, MgSO 4 0.2g / L, MnSO 4 0.05g / L, distilled water 1L, pH 6.2-6.4;

[0029] Step b, preparation of composite microecological preparation bacteria suspension: four kinds o...

Embodiment 2

[0040] Step a, preparation of compound microecological preparation seed liquid: use YPD medium to cultivate yeast (ACCC20165) at 30°C for 24h; LB broth medium to cultivate bacillus (CICC20037) at 30°C for 24h, and LB broth medium to cultivate photosynthetic bacteria at 30°C ( CCREMSDMCC150016) 48h; MRS culture medium 30 ℃ lactic acid bacteria (ACCC11016) 48h;

[0041] Wherein YPD medium, broth medium and MRS medium formulations are as follows:

[0042] LB broth medium: tryptone 10g / L, yeast extract powder 5g / L, NaCl 10g / L, distilled water 1L, pH6.8-7.0;

[0043]YPD medium: glucose 20g / L, peptone 20g / L, yeast extract 10g / L, distilled water 1L;

[0044] MRS medium: glucose 20g / L, peptone 10g / L, beef extract 10g / L, yeast extract 5g / L, anhydrous sodium acetate 5g / L, K 2 HPO 4 2g / L, triammonium citrate 2g / L, MgSO 4 0.2g / L, MnSO 4 0.05g / L, distilled water 1L, pH 6.2-6.4;

[0045] Step b, preparation of composite microecological preparation bacteria suspension: Bacillus CICC...

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Abstract

The invention relates to an immobilization technique for preparing a PVA-SA-zeolite powder copolymer microsphere coating from sodium alginate (SA) and polyvinyl alcohol (PVA) as raw materials, calcium chloride and boric acid as crosslinking agents together with a small amount of modified zeolite powder as a carrier and a composite microecologiccal preparation bacterial liquid. The production process provided by the invention is simple and easy to operate, efficient, safe and degradable in production raw material and easy to popularize and use. The process is applied to holothurian culture, the water purification effect of a composite microecologiccal preparation can be improved, the water purification time can be prolonged, the composite microecologiccal preparation can be easily preserved and transported when being compared with a bacterial liquid product mode, and the problems that the microecologiccal preparation is short in effect taking cycle and inconvenient to transport and preserve in the application process can be solved.

Description

technical field [0001] The invention relates to an immobilization technology, in particular to a method for preparing copolymer gel microspheres by immobilizing a composite microbial ecological preparation. Background technique [0002] At present, the compound micro-ecological preparation has been used in aquaculture, mainly in the sea cucumber aquaculture industry. It can be directly splashed into the culture water body, and can also be used as a feed additive. It can purify the water quality of the culture water body, promote the growth of sea cucumber, improve the intestinal The role of digestive enzyme activity in the tract. However, in practical application, there are problems of inconvenient transportation, short shelf life and unstable effect. Contents of the invention [0003] In order to solve the above problems, the present invention adopts a solution blending method to use sodium alginate (SA) and polyvinyl alcohol (PVA) as raw materials, calcium chloride and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/14C12N11/10C12N11/084C12N11/04C12R1/865C12R1/125C12R1/25C12R1/01
CPCC12N1/14C12N11/04C12N11/08C12N11/10
Inventor 王际辉肖珊陆璐
Owner DONGGUAN UNIV OF TECH
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