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Shiitake culture and method for simply preparing shiitake mycelium with same

A technology for mycelium and shiitake mushrooms, which is applied in the field of microbial fermentation, can solve the problems such as difficulty in subsequent utilization of mycelium and polysaccharide extraction and difficult separation, and achieve the effects of being convenient for direct consumption or crushing and utilization, short production cycle and reduced production cost.

Inactive Publication Date: 2019-09-27
河北省微生物研究所有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The production of edible and medicinal fungi by fermentation, and the use of fermented liquid to replace fruiting bodies to extract active ingredients are currently research hotspots and new technical methods for efficient utilization of edible and medicinal fungi, but there is no method for obtaining a large amount of pure mushroom mycelium. According to reports, Lei Sexiang et al. "A Lentinus Mushroom Powder" and Zhao Jingjing et al. "A Production Method of Lentinus Mushroom Mycelium for Extracting Lentinan" both use solid medium for cultivation, and the final product contains mycelium and medium mixed together , closely combined and difficult to separate, which brings great difficulties to the subsequent utilization of mycelium and polysaccharide extraction

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0038] A shiitake mushroom strain is classified as Lentinus edodes, and the preservation number is CGMCC NO.16991.

[0039] The method for simply preparing mushroom mycelium by utilizing Lentinus edodes CGMCC NO.16991 comprises the following process steps:

[0040] A. Submerged aerated fermentation: Inoculate the bacteria into the sterilized fermentation medium in the fermenter according to the volume ratio of 3% inoculum, and the fermentation is not low under the condition of aeration rate of 0.5:1 and fermentation temperature of 23°C In 5 days, the wet weight of the mycelium was determined to be no less than 18g / L, and the fermentation was completed to obtain the submerged culture fermentation liquid; the strain used Lentinus edodes CGMCCNO. 10 mg of PDA medium is added with vitamin B 1 ;

[0041]B, tissue culture bottle static fermentation: place the mycelium-producing medium in the tissue culture bottle, inoculate the submerged culture fermentation liquid in step A after...

Embodiment 2

[0058] The difference between this embodiment and embodiment 1 is:

[0059] The method for simply preparing mushroom mycelium by utilizing Lentinus edodes CGMCC NO.16991 comprises the following process steps:

[0060] A. Submerged aerated fermentation: Inoculate the bacteria into the sterilized fermentation medium in the fermenter according to the volume ratio of 10% inoculum, and the fermentation is not low under the condition of aeration rate of 0.7:1 and fermentation temperature of 30°C In 5 days, the wet weight of the mycelium was determined to be no less than 18g / L, and the fermentation was completed to obtain the submerged culture fermentation liquid; the strain used Lentinus edodes CGMCCNO. The amount of PDA medium 30mg added vitamin B 1 ;

[0061] B, tissue culture bottle static fermentation: place the mycelium-producing medium in the tissue culture bottle, inoculate the submerged culture fermentation liquid in step A after sterilization, and cultivate it for 20-30 d...

Embodiment 3

[0076] The difference between this embodiment and embodiment 1 is:

[0077] The method for simply preparing mushroom mycelium by utilizing Lentinus edodes CGMCC NO.16991 comprises the following process steps:

[0078] A. Submerged aerated fermentation: Inoculate the bacteria into the sterilized fermentation medium in the fermenter according to the volume ratio of 7% inoculum, and the fermentation is not low under the conditions of aeration rate of 0.6:1 and fermentation temperature of 25°C In 5 days, the wet weight of the mycelium was determined to be no less than 18g / L, and the fermentation was completed to obtain the submerged culture fermentation broth; the strain used was Lentinus edodes CGMCCNO.16991, and the fermentation medium was PDA medium without agar, according to the amount per 1000ml. Add 20 mg of PDA medium with agar and add vitamin B to it 1 ;

[0079] B, tissue culture bottle static fermentation: place the mycelium-producing medium in the tissue culture bottl...

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Abstract

The invention relates to microorganism fermentation, in particular to a shiitake culture and a method for simply preparing a shiitake mycelium with same. The method comprises the following process steps: inoculating a shiitake culture Lentinus edodes CGMCC NO. 16991 with a sterilized fermentation culture medium, and carrying out deep gas introduction fermentation so as to prepare a deep culture fermentation liquor; inoculating the deep culture fermentation liquor with a mycelium generation culture liquid, and carrying out tissue culture and bottle culture, so as to obtain the shiitake mycelium. The method is capable of solving problems that in the prior art a fermentation method is high in shiitake mycelium production investment, low in yield, high in technical difficulty, high in equipment investment, and the like, and has the advantages of being stable in product quality and process, low in production cost, and the like.

Description

technical field [0001] The invention relates to microbial fermentation, in particular to mushroom strains and a method for simply preparing mushroom mycelium by using the same. Background technique [0002] Lentinus edodes (Berk.) sing) is a large edible fungus, also known as flower mushroom, Xiangxin, Shiitake, winter wild mushroom, thick mushroom, and flower mushroom. Lentinus edodes has a wide range of cancer prevention and treatment, and has been used in clinical treatment. Shiitake mushrooms contain a variety of vitamins and minerals, which have a great effect on promoting human metabolism and improving the body's adaptability. Shiitake mushrooms also have a therapeutic effect on diabetes, tuberculosis, infectious hepatitis, neuritis, etc., and can be used for indigestion, constipation, and weight loss. Many ancient books in China record that shiitake mushrooms "replenish qi and prevent hunger, treat wind and blood, and benefit stomach and food". Folks are used to hel...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01H15/00A01G18/40C12R1/645
CPCA01H15/00A01G18/40C12N1/14C12R2001/645C12N1/145
Inventor 陈文杰张瑞青韩韬章淑艳古述江马清河秦艳梅
Owner 河北省微生物研究所有限公司
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