Method for preparing full-thickness skin tissue engineering scaffold with gradient pore structure

A technology for tissue engineering scaffolds and full-thickness skin, applied in additive processing, medical science, prostheses, etc., can solve the problems of difficult to meet the requirements of multi-layer complex structures, difficult to achieve precise control of pore structure and personalized customization, etc. Achieve the effects of promoting vascularization and proliferation of fibroblasts, accelerating wound healing, and combining tightly

Active Publication Date: 2019-10-08
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Traditional scaffold forming methods such as particle leaching, phase separation, freeze-drying, gas foaming, fiber weaving, etc., are difficult to achieve precise control of the pore structure and personalized customization, making it difficult to meet the complex multi-layer structure of skin tissue engineering. requirements

Method used

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  • Method for preparing full-thickness skin tissue engineering scaffold with gradient pore structure
  • Method for preparing full-thickness skin tissue engineering scaffold with gradient pore structure
  • Method for preparing full-thickness skin tissue engineering scaffold with gradient pore structure

Examples

Experimental program
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Effect test

Embodiment 1

[0034] The full-thickness skin tissue engineering scaffold with gradient pore structure prepared in this example has two layers, namely the epidermis and the dermis, wherein the epidermis is made of chitosan / silica gel, i.e. CS- Si gel is made, and the dermis is made of chitosan / silicon dioxide / gelatin gel, that is, CS-Si-Gel gel is made; the preparation process of the support is as follows:

[0035] Chitosan-silica hybrid (CS-Si) gel preparation: 8g chitosan was dissolved in 4% acetic acid aqueous solution to prepare 8wt% chitosan solution; 2 TEOS solution, stirred to make it evenly mixed, the obtained sol was transferred to a barrel, and after defoaming, it was placed at room temperature for 48 hours to gel.

[0036] Gelatin-chitosan-silica hybrid (10Gel-CS-Si) gel preparation: 1.2g gelatin (Gel) was added into deionized water to prepare a concentration of 10wt% gelatin solution A, and 0.12g GPTMS was added to the above solution , stirred vigorously for 2h under 50°C water ba...

Embodiment 2

[0041] The preparation process of the full-thickness skin tissue engineering scaffold with gradient pore structure in this embodiment is as follows:

[0042] Chitosan-silica hybrid (CS-Si) gel preparation: 12g chitosan was dissolved in 10% acetic acid aqueous solution to prepare 12wt% chitosan solution; 2 TEOS solution, stirred to make it evenly mixed, the obtained sol was transferred to a barrel, and after defoaming, it was placed at room temperature for 48 hours to gel.

[0043] Gelatin-chitosan-silica hybrid (20Gel-CS-Si) gel preparation: 2g gelatin (Gel) was added to 8g deionized water to prepare a concentration of 20wt% gelatin solution A, and 0.2g was added to the above solution GPTMS, stirred vigorously in a water bath at 50°C for 2 hours; weighed 12g of chitosan (CS) and dispersed it in deionized water to prepare 8wt% chitosan suspension B; weighed TEOS solution and dissolved it in an acidic deionized In the aqueous solution, the TEOS hydrolyzate C was prepared.

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Embodiment 3

[0048] The preparation process of the full-thickness skin tissue engineering scaffold with gradient pore structure in this embodiment is as follows:

[0049] Chitosan-silica hybrid (CS-Si) gel preparation: 8g chitosan is dissolved in 4% acetic acid aqueous solution and prepares the chitosan solution of 8wt%; Add the TEOS solution of pre-hydrolysis, stir to make it Mix evenly, transfer the resulting sol into a barrel, remove the bubbles and let it gel at room temperature for 2 hours.

[0050] Gelatin-chitosan-silica hybrid (10Gel-CS-Si) gel preparation: 2g gelatin (Gel) was added into deionized water to prepare a concentration of 10wt% gelatin solution A, and 0.6g GPTMS was added to the above solution, Stir vigorously in a water bath at 50°C for 2 hours; weigh 12g of chitosan (CS) and disperse it in deionized water to prepare 8wt% chitosan suspension B; measure TEOS solution and dissolve it in an acidic deionized aqueous solution with a pH value of 2 , to prepare TEOS hydrolyz...

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Abstract

The invention relates to the technical field of tissue engineering skin, and concretely relates to a method for preparing a full-thickness skin tissue engineering scaffold with a gradient pore structure. The organic-inorganic hybrid materials are prepared by a sol-gel technique. According to the structure and composition characteristics of skin tissue, a CS-Si-Gel component is selected as a dermislayer and a CS-Si component is used as an epidermal layer, and the full-thickness skin tissue engineering scaffold with the gradient pore structure is successfully constructed by using a multi-nozzleprinting system. The material selected by the invention has excellent biocompatibility, antibacterial property, and the like, and the prepared scaffold has the advantage of structural bionics. In addition, a 3D scanning modeling technology is used for layered printing, stacking layer by layer is carried out, which can be customized, so that the success rate of full-thickness skin defect repair can be increased. The skin tissue engineering scaffold can also be used for in vitro skin model research and plays an important role in drug and cosmetic testing as well as on cancer research.

Description

technical field [0001] The invention relates to the technical field of tissue engineering skin, in particular to a method for preparing a full-thickness skin tissue engineering scaffold with a gradient pore structure. Background technique [0002] As the largest organ of the human body, skin can be divided into epidermis, dermis and subcutaneous tissue in anatomical structure, and contains accessory organs (sweat glands, skin sacs), blood vessels, lymphatic vessels, muscles and nerves. Because it is exposed to the external environment in a large area, it is extremely vulnerable to various injuries, such as burns, surgical incisions, and ulcers caused by chronic diseases (diabetes, etc.), and the complex structure of the skin tissue makes the wound healing often extremely difficult. [0003] Although the currently used skin wound dressings can accelerate wound healing, it is difficult to repair and regenerate skin tissue in terms of structure and function for large-area and d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/60A61L27/50A61L27/58A61L27/56A61L27/20A61L27/02A61L27/22B33Y10/00B33Y70/00
CPCA61L27/025A61L27/20A61L27/222A61L27/50A61L27/56A61L27/58A61L27/60B33Y10/00B33Y70/00C08L5/08
Inventor 赵娜如许珊董怡帆王迎军
Owner SOUTH CHINA UNIV OF TECH
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