Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Monascus for high yield of saccharifying enzyme, esterifying enzyme and protease and isolated culture method and application thereof

A technology for separating, culturing and saccharifying enzymes, applied in the field of winemaking, can solve the problem of undisclosed Monascus, and achieve the effects of elegant and delicate aroma, typical style and soft taste

Active Publication Date: 2019-10-11
JIANGSU KINGS LUCK BREWERY
View PDF3 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There are many reports about screening functional Monascus in the prior art, but how to obtain a Monascus with high yield of glucoamylase, esterase and protease has not been disclosed, which is of great significance for improving the yield and product quality of liquor brewing

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Monascus for high yield of saccharifying enzyme, esterifying enzyme and protease and isolated culture method and application thereof
  • Monascus for high yield of saccharifying enzyme, esterifying enzyme and protease and isolated culture method and application thereof
  • Monascus for high yield of saccharifying enzyme, esterifying enzyme and protease and isolated culture method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] The present embodiment provides a kind of Monascus with high yield of glucoamylase, esterase and protease, and the isolation and cultivation method comprises the following steps:

[0056] (1) Grind 10 g of distiller's yeast with a sterile mortar and place it in a conical flask with 90 mL of sterile water, and shake it for 30 minutes to obtain a bacterial suspension;

[0057] (2) After diluting the bacterial suspension obtained in step (1) by 10 times, apply the rolling ball method on the aseptic bran juice solid medium and carry out inverted culture at 30° C. for 8 days to obtain colonies;

[0058] (3) pick the morphologically regular bacterium colony in the step (2) and streak culture on the aseptic bran juice solid medium, carry out 3 times of repeated operation separation and purification, obtain a single pure-bred bacterium colony, and microscopic examination is determined to be the bacterium of the present invention Involved Monascus with high production of glucoam...

Embodiment 2

[0063] The present embodiment provides a kind of Monascus with high yield of glucoamylase, esterase and protease, and the isolation and cultivation method comprises the following steps:

[0064] (1) Grind 10 g of distiller's yeast with a sterile mortar and place it in a conical flask with 90 mL of sterile water, and shake it for 30 minutes to obtain a bacterial suspension;

[0065] (2) After diluting the bacterial suspension obtained in step (1) by 10 times, apply the rolling ball method on the aseptic bran juice solid medium and carry out inverted culture at 20° C. for 10 days to obtain colonies;

[0066] (3) pick the morphologically regular bacterium colony in the step (2) and culture it by streaking on the aseptic bran juice solid medium, carry out 3 repeated operations to separate and purify, and obtain a single pure-bred bacterium colony, which is determined to be Monascus by microscopic examination, It is characterized by colonies that are white in the early stage of cul...

Embodiment 3

[0068] The present embodiment provides a kind of Monascus with high yield of glucoamylase, esterase and protease, and the isolation and cultivation method comprises the following steps:

[0069] (1) Grind 10 g of distiller's yeast with a sterile mortar and place it in a conical flask with 90 mL of sterile water, and shake it for 30 minutes to obtain a bacterial suspension;

[0070] (2) After diluting the bacterial suspension obtained in step (1) by 10 times, apply the rolling ball method on the aseptic bran juice solid medium and carry out inverted culture at 45° C. for 5 days to obtain colonies;

[0071] (3) pick the morphologically regular bacterium colony in step (2) and culture it by streaking on the aseptic bran juice solid medium, carry out 2 repeated operations for isolation and purification, and obtain a single pure-bred bacterium colony, which is determined to be Monascus by microscopic examination, It is characterized by colonies that are white in the early stage of ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to View More

Abstract

The invention relates to monascus for high yield of a saccharifying enzyme, an esterifying enzyme and protease and an isolated culture method and application thereof. The monascus for high yield of the saccharifying enzyme, the esterifying enzyme and the protease is named as a Monascus anka T4 strain, the preservation unit is China General Microbiological Culture Collection Center, the preservation time is 25th February, 2019, the preservation number is CGMCC No.17074, and the address is No.3 the first yard, the eastern Beichen road, Chaoyang district, Beijing. The monascus has the good characteristic of high yield of the saccharifying enzyme, the esterifying enzyme and the protease, the obtained anka saccharifying power can reach up to 2,080 mg / (g h), the protease activity can reach up to9.7 microgram / (g min), and the esterifying power can reach up to 38.6 mg / (g 100h). The strain is of the great significance in increasing the baijiu brewing liquor yield and improving the product quality.

Description

technical field [0001] The invention belongs to the technical field of brewing, and in particular relates to a monascus and its isolation and cultivation method and application, in particular to a monascus with high production of glucoamylase, esterase and protease, its isolation and cultivation method and application. Background technique [0002] The use of Monascus has a long history in our country. As early as the Ming Dynasty, my country used it to cultivate red yeast rice as a raw material for medicinal purposes, wine making, vinegar making, fermented bean curd or pickled meat. The modern fermentation industry uses them to produce glucose, alcoholic fermentation and red yeast rice pigments, as well as a series of functional red yeast rice products such as regulating blood lipids and lowering blood pressure. People can use Monascus pigments to color and preserve food, and Monascus can also be used to make wine. Monascus can produce glucoamylase, esterase, protease and o...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/02C12G3/02C12R1/66
CPCC12G3/02C12N1/02C12N1/14C12N1/145C12R2001/66
Inventor 吴建峰季方张振坤陈尚一杨艳
Owner JIANGSU KINGS LUCK BREWERY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com