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Caffeine removal method in Ginkgo biloba extract

An extract and technology of ginkgo biloba, applied in the field of removal of harmful substances in ginkgo biloba extract, can solve the problem of low caffeine ginkgo biloba extract, etc., and achieve the effect of low cost and simple process

Active Publication Date: 2021-04-09
CHENGUANG BIOTECH GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the literature on the refining of Ginkgo biloba extract is mainly aimed at the purification of flavonoids and ginkgolide B in Ginkgo biloba extract, and there are almost no literature reports on the purification of low-caffeine Ginkgo biloba extract.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Add 30L of hydrochloric acid aqueous solution at 15°C and pH=5.7 to 20kg of ginkgo leaf extract, stir at 90r / min for 2h, and centrifuge with a decanter centrifuge with a centrifugal factor of not less than 6000 to obtain 14L of aqueous acid solution I , 26.3kg viscous substance I;

[0020] (2) After vacuum-drying the obtained viscous substance I at 53°C and vacuum degree of -0.085MPa, 17.7kg of Ginkgo biloba extract I was obtained;

[0021] (3) Add 3 times the volume of dichloromethane to the acid aqueous solution I, extract 3 times at 34°C, stand to separate and separate, and carry out organic solvent recovery at 35°C and a vacuum of -0.04MPa for the solvent phase of the lower layer, and the upper layer of water The phases were combined to obtain solution II;

[0022] (4) Add sodium hydroxide solution with a mass fraction of 5‰ to the obtained solution II to adjust the pH value to 9.0, and heat it to 35°C and pass it through a DM-130 macroporous adsorption resin c...

Embodiment 2

[0027] (1) Add 40L of phosphoric acid aqueous solution with a temperature of 10°C and pH=5.8 to 20kg of ginkgo leaf extract, stir at 90r / min for 0.5h, and then centrifuge with a tubular centrifuge with a centrifugal factor of not less than 6000 to obtain 33L of aqueous acid solution 1, 26kg viscous substance I;

[0028] (2) After vacuum-drying the obtained viscous substance I at 53°C and a vacuum degree of -0.08MPa, 17.8kg of Ginkgo biloba extract I was obtained;

[0029] (3) Add 4 times the volume of dichloromethane to the acid aqueous solution I, extract 4 times at 32°C, stand to separate and separate, and carry out organic solvent recovery at 31°C and vacuum degree of -0.04MPa for each lower solvent phase obtained, and the upper layer of water The phases were combined to obtain solution II;

[0030] (4) Add sodium bicarbonate solution with a mass fraction of 5‰ to the obtained solution II to adjust the pH value to 9.5, heat it to 36°C, pass through LSA-10 macroporous adsor...

Embodiment 3

[0035] (1) Add 45L of hydrochloric acid aqueous solution with a temperature of 15°C and pH=6.0 to 20kg of ginkgo leaf extract, stir at 90r / min for 3h, and centrifuge with a disc centrifuge with a centrifugal factor of not less than 6000 to obtain 37L of aqueous acid solution 1, 26.2kg viscous substance I;

[0036] (2) The obtained viscous substance I was vacuum-dried at a temperature of 55°C and a vacuum degree of -0.09MPa to obtain 17.6kg of Ginkgo biloba extract I;

[0037] (3) Solution I was extracted by adding 4 times the volume of dichloromethane at 36°C for 4 times, standing to separate layers, and the solvent phase of the lower layer was collected at 33°C and vacuum degree -0.05MPa for each pass, and the upper layer of the aqueous phase was combined to obtain Solution II;

[0038] (4) Add sodium hydroxide solution with a mass fraction of 5‰ to the obtained solution II to adjust the pH value to 10.0, heat it to 40°C and pass through LSA-20 macroporous adsorption resin a...

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PUM

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Abstract

A method for removing caffeine in ginkgo leaf extract, comprising the following steps: (1) adding an acidic aqueous solution with a pH value of 5.7 to 6.0 at 10 to 15°C to the ginkgo leaf extract, and mixing at 10 to 15°C After centrifugation, acid aqueous solution I and viscous substance I were obtained; (2) viscous substance I was vacuum-dried to obtain ginkgo biloba extract I with low caffeine content; (3) dichloromethane was added to acid aqueous solution I to extract 3-5 (4) Adjust the pH value of solution II to 9.0-10.0, pass through the macroporous adsorption resin after heating, and then rinse the resin column with hot water until the pH value is 6.5-7.5 Analyzing, removing the solvent residue from the analyzing solution, and obtaining Ginkgo biloba extract II with low caffeine content; (5) Ginkgo biloba extract I and II are thoroughly mixed to obtain the caffeine-removed Ginkgo biloba extract. The invention has simple process, low cost and is suitable for industrialized production.

Description

technical field [0001] The invention relates to a method for removing harmful substances in ginkgo leaf extract, in particular to a method for removing caffeine in ginkgo leaf extract. Background technique [0002] Ginkgo biloba L. is a deciduous tree of the genus Ginkgo in the Ginkgo family, and is a special plant in my country. Ginkgo biloba is an important medicinal part of Ginkgo biloba. Its main active ingredients are flavonoids and lactones. Among them, flavonoids can resist oxidation, improve cardiovascular and cerebrovascular circulation, and have anti-virus, anti-aging, anti-allergic, anti-tumor, Lower cholesterol, regulate the body's immune system and other effects. Ginkgo biloba has been used as medicine for more than 60 years. Ginkgo biloba extract (GBE) is the first-line drug for clinical treatment of cerebrovascular diseases. The effect of dampness and antidiarrheal is mainly used to treat coronary heart disease, cough disease, hyperlipidemia, angina pectoris...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/16A61K127/00
CPCA61K36/16A61K2236/333A61K2236/51A61K2236/53A61K2236/55
Inventor 张玉芬张苹苹张晓芳刘登帅程远欣
Owner CHENGUANG BIOTECH GRP CO LTD
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