Extracellular glucose oxidase-producing strain CY-12 and application thereof
A technology of glucose oxidase and CY-12, applied in the field of microorganisms, can solve the problems of lack of high-yielding strains, complicated separation and purification processes, single production strains, etc., and achieves the effects of simple preparation process, simple obtaining method and high recovery rate.
Active Publication Date: 2019-10-22
HENAN ACAD OF SCI INST OF BIOLOGY LIABILITY
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Problems solved by technology
[0004] In view of the above situation, in order to solve the defects of the prior art, the purpose of the present invention is to provide a kind of extracellular glucose oxidase producing strain CY-12 and its application, which can effectively solve the problem of single production strain, lack of high-yield strain, The problem of complex separation and purification process
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[0019] The specific implementation manners of the present invention will be described in further detail below in conjunction with the accompanying drawings and actual conditions.
[0020] The source of the extracellular glucose oxidase-producing strain CY-12 of the present invention is obtained by separating and purifying the soil samples of the vineyard soil in the Changyuan Yellow River beach in Xinxiang.
[0021] The relevant experimental data are as follows:
[0022] 1. Screening and identification of strains producing extracellular glucose oxidase
[0023] Soil samples collected from vineyards in Changyuan Yellow River Beach, Xinxiang, were spread on primary screening plate medium by conventional gradient dilution method, cultured at 28°C until colonies grew, placed in a 4°C refrigerator, and observed for color development.
[0024] The primary screening plate medium (g / L): glucose 80, peptone 3, (NH 4 ) 2 HPO 4 0.4, KH 2 PO 4 0.2, MgSO 4 •7H 2 O 0.2, CaCO 3 3...
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The invention relates to an extracellular glucose oxidase-producing strain CY-12 and application thereof. Problems in the prior art, such as single producing strain, lack of high-producing strain andcomplex separation and purification process can be effectively solved. According to the technical scheme, the extracellular glucose oxidase-producing strain CY-12 has the classification name of Cladosporium sp. and was preserved in the China General Microbiological Culture Collection Center on April 19, 2019 with the preservation number of CGMCC NO. 17661. The strain of the invention has excellentproperties; the method for obtaining the produced extracellular glucose oxidase is simple and easy to operate; the recovery rate is high; the preparation process is simple and efficient, is energy-saving and environmentally-friendly, and can provide a new glucose oxidase variety for the researchers in the application industry at home, has great application potential in the fields of food, medicaltreatment, feed industry and the like, and is an innovation for the glucose oxidase strain.
Description
technical field [0001] The invention relates to the field of microorganisms, in particular to a strain CY-12 producing extracellular glucose oxidase and its application. Background technique [0002] Glucose oxidase (GOD) is an aerobic dehydrogenase that can highly specifically catalyze β-D-glucose to generate gluconic acid and hydrogen peroxide in the presence of oxygen Galactose and xylose also oxidize more slowly. At present, the enzyme is widely used in food, feed, medicine and other fields. In the food industry, glucose and oxygen in food can be removed, thereby eliminating the Maillard reaction, effectively inhibiting non-enzymatic browning, and prolonging the shelf life; hydrogen peroxide catalyzed by glucose oxidase has a bactericidal effect and is used as a bacteriostatic agent Adding it to food is safer than chemical antibacterial agents; in the pharmaceutical industry, glucose oxidase is a catalyst for the production of gluconic acid, which can catalyze the prod...
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IPC IPC(8): C12N1/14C12N1/02C12N9/04C12R1/645
CPCC12N1/14C12N1/02C12N9/0006C12Y101/03004C12R2001/645C12N1/145
Inventor 权淑静解复红胡虹陈国参刁文涛张英涛张秀江王秋菊向凌云刘丽冯菲
Owner HENAN ACAD OF SCI INST OF BIOLOGY LIABILITY