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Pseudoarthrobacter and application thereof, and biological organic fertilizer and application thereof

A bio-organic fertilizer and Arthrobacter technology, applied in the field of fertilizers, can solve the problems of reduced fertilizer efficiency, reproduction of harmful bacteria, and low utilization rate, and achieve the effect of increasing yield and significantly promoting growth

Active Publication Date: 2019-10-22
INST OF SOIL FERTILIZER & WATER SAVING AGRI GANSU ACAD OF AGRI SCI
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

With the increase of artificial planting area and the extension of continuous cropping years, the imbalance of soil microbial flora, the reproduction of harmful flora, and the increase of soil-borne diseases, coupled with the long-term application of chemical fertilizers during the cultivation of Chinese medicinal materials, resulting in a decline in fertilizer efficiency and low utilization rate , soil compaction and other disadvantages, which significantly reduce the yield of Chinese herbal medicines

Method used

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  • Pseudoarthrobacter and application thereof, and biological organic fertilizer and application thereof
  • Pseudoarthrobacter and application thereof, and biological organic fertilizer and application thereof
  • Pseudoarthrobacter and application thereof, and biological organic fertilizer and application thereof

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preparation example Construction

[0027] In the present invention, the pseudoarthrobacter preferably adopts a pseudoarthrobacter fermentation liquid, and the preparation method of the pseudoarthrobacter fermentation liquid is preferably to inoculate the pseudoarthrobacter into a pseudoarthrobacter fermentation medium for cultivation to obtain a pseudoarthrobacter fermented liquid. The pseudoarthrobacter fermentation medium uses water as a solvent, preferably comprising: 12 g of starch, 4.8 g of yeast powder, 0.8 g of dipotassium hydrogen phosphate, 1 g of magnesium sulfate heptahydrate and 3 g of calcium carbonate per liter. The pH value of the fermentation medium is preferably 6.5-8.0, more preferably 6.7. The culture condition is preferably 20-35° C. for 28-40 hours of fermentation. The number of effective viable bacteria in the fermentation broth is preferably greater than 1 billion.

[0028] The bio-organic fertilizer of the present invention comprises 25-42.5 parts by mass of humic acid coal, preferably...

Embodiment 1

[0035] Preparation of Pseudoarthrobacter DG-22 liquid bacterial agent: get the purified bacterial strain DG-22 under aseptic conditions, use the inoculation loop to dip 3 rings and transfer to the Erlenmeyer flask with 250 milliliters of sterile liquid medium, Shake at a constant temperature (190 rpm) at 30°C for 4 days, and check the purity and quantity of the strains under a microscope before use. The effective number of viable bacteria in Pseudoarthrobacter DG-22 liquid bacterial agent is 1×10 9 cfu / ml.

[0036] The experiment was conducted in the greenhouse of the Gansu Academy of Agricultural Sciences, and was carried out through pot experiments, and the test pots used small plastic flower pots. Each pot contains 1.1Kg of substrate, and the substrate formula is soil:imported peat:vermiculite=1:0.1:0.1. Weigh 1.1kg of substrate before transplanting, and water 400ml per pot when starting to sow. Sow 30 Astragalus seeds in each pot, inoculate 30ml of DG-22 liquid inoculum...

Embodiment 2

[0042] After uniformly mixing 42.5g of humic acid coal and 42.5g of cow dung, continue mixing with 15g of pseudoarthrobacter, and ferment for 2 days to obtain bio-organic fertilizer. Among them, the preservation number of pseudoarthrobacter is CGMCC No.17858, and the effective number of viable bacteria of pseudoarthrobacter is 1×10 9 cfu / g, the effective number of viable bacteria in bio-organic fertilizer is 500 million cfu / g.

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Abstract

The invention provides pseudoarthrobacter and a biological organic fertilizer and application thereof and relates to the technical field of fertilizers. The pseudoarthrobacter has the Latin name Pseudomonas sp. with the preservation number CGMCC No.17858. The pseudoarthrobacter sp. DG-22 has the remarkable growth promoting effect on traditional Chinese medicinal materials. The biological organic fertilizer prepared by utilizing the pseudoarthrobacter can effectively increase the yield of traditional Chinese medicinal materials and improve the activities of soil urease and acid phosphatase.

Description

technical field [0001] The invention relates to the technical field of fertilizers, in particular to a pseudoarthrobacter, bio-organic fertilizer and applications thereof. Background technique [0002] Astragalus mongholicus is a leguminous plant with high medicinal value. Its root is one of the commonly used Chinese medicinal materials in my country. It is mainly produced in Inner Mongolia, Shanxi, Gansu, Heilongjiang and other places. Yang, solid surface antiperspirant, detoxification and drainage of pus, diuresis, swelling and granulation and other effects. [0003] Angelica sinensis, whose root can be used as medicine, is one of the most commonly used traditional Chinese medicines. It has the effects of nourishing and harmonizing blood, regulating menstruation and relieving pain, moistening dryness and smoothing intestines, anti-cancer, anti-aging, and immunity. [0004] Codonopsis pilosula (Franch.) Nannf. Codonopsis pilosula (Franch.) Nannf.) Codonopsis pilosula (Fran...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05G3/00C05G3/04C12R1/01
CPCC12N1/20C05G3/00C05F3/00C12R2001/01C12N1/205C05G3/80Y02W30/40
Inventor 李娟王文丽赵旭
Owner INST OF SOIL FERTILIZER & WATER SAVING AGRI GANSU ACAD OF AGRI SCI