Pediococcus acidilactici micro-capsule and preparation method thereof

A technology of Pediococcus lactis and microcapsules, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of metabolite loss, loss of activity, bacterial death, etc., and achieve simple post-treatment process and reduce The effect of wastewater discharge and bacterial activity maintenance

Pending Publication Date: 2019-10-25
日照东润德农牧发展有限公司
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because Pediococcus lactis has high requirements on nutritional status and nutritional conditions, and the final concentration of growth bacteria is not high
In addition, Pediococcus lactis is not resistant to high temperature and is dried by freeze-drying. The product has high activity but requires high cost and equipment investment.
Spray drying and fluidized bed drying methods require less investment in equipment, but it is easy to cause bacterial death and loss of activity. Fluidized bed drying technology requires post-processing techniques such as centrifugation to separate the solid and liquid of the bacterial cells in the fermentation broth, which not only makes the fermentation broth The metabolites in the water are lost in vain, and cause waste water discharge

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Pediococcus acidilactici micro-capsule and preparation method thereof
  • Pediococcus acidilactici micro-capsule and preparation method thereof
  • Pediococcus acidilactici micro-capsule and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A Pediococcus lactis microcapsule is prepared from the following components in parts by weight:

[0031]

[0032] Wherein the sodium alginate solution is an aqueous solution of sodium alginate with a weight fraction of 3.375%, the polyethylene glycol solution is an aqueous solution of polyethylene glycol with a mass fraction of 2%, and the calcium chloride solution is a 20% calcium chloride solution with a mass fraction of The aqueous solution of calcium, the chitosan solution is the aqueous solution of chitosan that the mass fraction is 2%, and the thalline number of Pediococcus lactis in the Pediococcus lactis seed liquid is 10 8 CFU / mL.

[0033] A kind of preparation method of Pediococcus lactis microcapsule according to above-mentioned formula comprises the following steps:

[0034] S1 Dissolve sodium alginate powder and polyethylene glycol liquid in water, mix the prepared sodium alginate solution and polyethylene glycol solution evenly to obtain a mixed soluti...

Embodiment 2

[0044] A Pediococcus lactis microcapsule is prepared from the following components in parts by weight:

[0045]

[0046] Wherein the sodium alginate solution is an aqueous solution of sodium alginate with a weight fraction of 3.75%, the polyethylene glycol solution is an aqueous solution of polyethylene glycol with a mass fraction of 4%, and the calcium chloride solution is a 40% sodium chloride solution with a mass fraction of The aqueous solution of calcium, the chitosan solution is the aqueous solution of the chitosan that mass fraction is 4%, the thalline number of the Pediococcus lactis in the Pediococcus lactis seed liquid is 10 9 CFU / mL.

[0047] A kind of preparation method of Pediococcus lactis microcapsule according to above-mentioned formula comprises the following steps:

[0048] S1 Dissolve sodium alginate powder and polyethylene glycol liquid in water, mix the prepared sodium alginate solution and polyethylene glycol solution evenly to obtain a mixed solution...

Embodiment 3

[0058] A Pediococcus lactis microcapsule is prepared from the following components in parts by weight:

[0059]

[0060] Wherein the sodium alginate solution is an aqueous solution of 3% sodium alginate by weight fraction, the polyethylene glycol solution is an aqueous solution of polyethylene glycol with a mass fraction of 1.33%, and the calcium chloride solution is 13.3% calcium chloride solution with a mass fraction of The aqueous solution of calcium, the chitosan solution is the aqueous solution of the chitosan that mass fraction is 1.33%, the thalline number of Pediococcus lactis in the Pediococcus lactis seed liquid is 10 8 CFU / mL.

[0061] A kind of preparation method of Pediococcus lactis microcapsule according to above-mentioned formula comprises the following steps:

[0062] S1 Dissolve sodium alginate powder and polyethylene glycol liquid in water, mix the prepared sodium alginate solution and polyethylene glycol solution evenly to obtain a mixed solution, put t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
porosityaaaaaaaaaa
quality scoreaaaaaaaaaa
Login to view more

Abstract

The invention provides a pediococcus acidilacticimicro-capsule and a preparation method thereof, and relates to the field of non-antibiotic feed additives and preparation methods thereof. The pediococcus acidilacticimicro-capsule is a 40-60-micrometer micro-capsule prepared by conducting microencapsulation on a seed solution of pediococcus acidilactici through sodium alginate and polyethylene glycol, then high-density liquid fermentation culture is conducted in a fermentation tank, and finally a preparation is obtained through spray drying. According to the pediococcus acidilacticimicro-capsule, pediococcus acidilactici can grow and multiply in quantity in the capsule wall, the capsulewall of the micro-capsule has reticulated pores, mass transfer is not affected, medium components can freely enter and exit, the treatment process is simple, the production is continuous, and the pediococcus acidilactici is better protected, the thallus is resistant to high temperature, the activity of the thallus is effectively kept, it can be guaranteed that fermentation metabolites are not lost, the wastewater discharge is reduced, the product cost is further lowered, and the thallus activity is kept at90% or above. The products of the company are more competitive in the market.

Description

technical field [0001] The invention relates to the field of non-antibiotic feed additives and a preparation method thereof, in particular to a Pediococcus lactis microcapsule and a preparation method thereof. Background technique [0002] There are more than 100 known species of lactic acid bacteria. The application history of lactic acid bacteria is very long, such as the production and production of dairy products, pickles, silage, etc. There are a large number of lactic acid bacteria in the intestines of humans and animals. Lactic acid bacteria preparation is a preparation containing live lactic acid bacteria called Lactobacillus. Lactic acid bacteria grow and develop in the intestinal tract, decompose sugars to generate lactic acid and form an acidic environment, thereby inhibiting the proliferation of harmful bacteria in the intestinal tract, and preventing toxin production and disease-causing. The strains for preparing the lactic acid bacteria preparation include L...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N11/08C12N11/10C12R1/01
CPCC12N1/20C12N11/08C12N11/10
Inventor 郝永任张冬梅郭衍涛陈守慧贾衍俊
Owner 日照东润德农牧发展有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products