Pediococcus acidilactici micro-capsule and preparation method thereof
A technology of Pediococcus lactis and microcapsules, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of metabolite loss, loss of activity, bacterial death, etc., and achieve simple post-treatment process and reduce The effect of wastewater discharge and bacterial activity maintenance
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Embodiment 1
[0030] A Pediococcus lactis microcapsule is prepared from the following components in parts by weight:
[0031]
[0032] Wherein the sodium alginate solution is an aqueous solution of sodium alginate with a weight fraction of 3.375%, the polyethylene glycol solution is an aqueous solution of polyethylene glycol with a mass fraction of 2%, and the calcium chloride solution is a 20% calcium chloride solution with a mass fraction of The aqueous solution of calcium, the chitosan solution is the aqueous solution of chitosan that the mass fraction is 2%, and the thalline number of Pediococcus lactis in the Pediococcus lactis seed liquid is 10 8 CFU / mL.
[0033] A kind of preparation method of Pediococcus lactis microcapsule according to above-mentioned formula comprises the following steps:
[0034] S1 Dissolve sodium alginate powder and polyethylene glycol liquid in water, mix the prepared sodium alginate solution and polyethylene glycol solution evenly to obtain a mixed soluti...
Embodiment 2
[0044] A Pediococcus lactis microcapsule is prepared from the following components in parts by weight:
[0045]
[0046] Wherein the sodium alginate solution is an aqueous solution of sodium alginate with a weight fraction of 3.75%, the polyethylene glycol solution is an aqueous solution of polyethylene glycol with a mass fraction of 4%, and the calcium chloride solution is a 40% sodium chloride solution with a mass fraction of The aqueous solution of calcium, the chitosan solution is the aqueous solution of the chitosan that mass fraction is 4%, the thalline number of the Pediococcus lactis in the Pediococcus lactis seed liquid is 10 9 CFU / mL.
[0047] A kind of preparation method of Pediococcus lactis microcapsule according to above-mentioned formula comprises the following steps:
[0048] S1 Dissolve sodium alginate powder and polyethylene glycol liquid in water, mix the prepared sodium alginate solution and polyethylene glycol solution evenly to obtain a mixed solution...
Embodiment 3
[0058] A Pediococcus lactis microcapsule is prepared from the following components in parts by weight:
[0059]
[0060] Wherein the sodium alginate solution is an aqueous solution of 3% sodium alginate by weight fraction, the polyethylene glycol solution is an aqueous solution of polyethylene glycol with a mass fraction of 1.33%, and the calcium chloride solution is 13.3% calcium chloride solution with a mass fraction of The aqueous solution of calcium, the chitosan solution is the aqueous solution of the chitosan that mass fraction is 1.33%, the thalline number of Pediococcus lactis in the Pediococcus lactis seed liquid is 10 8 CFU / mL.
[0061] A kind of preparation method of Pediococcus lactis microcapsule according to above-mentioned formula comprises the following steps:
[0062] S1 Dissolve sodium alginate powder and polyethylene glycol liquid in water, mix the prepared sodium alginate solution and polyethylene glycol solution evenly to obtain a mixed solution, put t...
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