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A method for extracting and separating S-adenosylmethionine from S-adenosylmethionine fermentation broth

A technology of adenosylmethionine and fermentation broth, which is applied in the field of bioengineering, can solve the problems of poor stability, transformation and degradation of S-adenosylmethionine, high cost of S-adenosylmethionine, and impurity ash in products, etc. Simple and reliable effect with low product loss rate

Active Publication Date: 2020-05-12
山东惠仕莱生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] To sum up, using the traditional method to separate and purify S-adenosylmethionine has high cost, long separation process, low product yield, high cost, and poor stability of S-adenosylmethionine, which is prone to transformation and degradation during the long-term separation process. It is easy to cause the product impurities and ash content to be too high

Method used

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  • A method for extracting and separating S-adenosylmethionine from S-adenosylmethionine fermentation broth
  • A method for extracting and separating S-adenosylmethionine from S-adenosylmethionine fermentation broth

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The fermentation broth was passed through a disc centrifuge at a speed of 3000rpm to collect the yeast, and 30% of the volume of the fermentation broth was added to the pure water to wash the bacteria. Adjust the bacterial concentration to 10% and enter the starch injection liquefaction device. Instantaneous high temperature breaks the wall. The injection temperature is 105 ° C. After 30 seconds after injection, the feed liquid is cooled to 40 ° C. The cell fragmentation rate is 99.2%, and the yield of S-adenosylmethionine is 98.5. %; Pass the broken wall liquid through a microfiltration membrane with a pore size of 500 μm, collect the filtrate, and add 30% volume of pure water to wash. Pass the microfiltration dialysate through the ultrafiltration membrane (the material is PVDF, the molecular weight cut-off is 6000D), add pure water with 30% of the volume of the feed solution to clean the ultrafiltration membrane, and the concentration of S-adenosylmethionine in the ult...

Embodiment 2

[0024] The fermented liquid is passed through a disc centrifuge at a speed of 5000 rpm, the yeast is collected, and 50% of the volume of the fermented liquid is added to wash the cells with pure water. Adjust the bacterial concentration to 50% and enter the starch injection liquefaction device. Instantaneously high temperature breaks the wall. The injection temperature is 110°C. After 30 seconds of injection, the feed liquid is cooled to 45°C. The cell fragmentation rate is 99.6%, and the yield of S-adenosylmethionine is 96.5% %; Pass the broken wall solution through a microfiltration membrane with a pore size of 200 μm, collect the filtrate, and add 50% volume of pure water to wash. Pass the microfiltration dialysate through the ultrafiltration membrane (the material is PVDF, the molecular weight cut-off is 8000D), add pure water with 30% of the feed volume to clean the ultrafiltration membrane, and the concentration of S-adenosylmethionine in the ultrafiltration dialysate is ...

Embodiment 3

[0027] Comparison of Various Yeast Wall Breaking Methods

[0028] The fermented liquid passes through a disc centrifuge at a speed of 6000rpm, collects yeast, and adds 50% pure water of the fermented liquid volume to wash the thallus. Adjust the bacterial concentration to 30%. The yeast cells were disrupted in quadruplicate by high-temperature instantaneous wall breaking method, ultrasonic method, hot water wall breaking method, and dilute sulfuric acid wall breaking method. Compare the wall breaking rate, S-adenosylmethionine yield and S-S isomer ratio. The results are entered in the table below:

[0029]

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Abstract

The invention provides a method for extracting and separating S-adenosylmethionine from S-adenosylmethionine fermentation liquor, so that the total yield of the S-adenosylmethionine reaches 70% or above. The method comprises the following steps of: a, wall breaking: subjecting fermentation liquor to a disc centrifuge, collecting saccharomycetes, adding pure water-washed thallus which is 30-100% byvolume of the fermentation liquor, subjecting the obtained bacterial liquid to a starch jet liquefaction device, and performing rapid cooling after instant high-temperature wall breaking to enable the cell breaking rate to be 99% or above; b, crude extraction: passing the wall-broken liquid obtained in the step a through a microfiltration membrane with the aperture of 50-200 [mu]m, collecting filtrate, controlling the interception rate of S-adenosylmethionine by the microfiltration membrane to be less than or equal to 5%, passing the dialysate through an ultrafiltration membrane (the materialis PVDF, the interception molecular weight is 3000-10000D), and removing macromolecular substances such as proteins to obtain a crude extract of the S-adenosylmethionine; c, refining: purifying the crude extract obtained in the step b by using weak acid type cation exchange resin, and decolorizing the eluent by using decolorizing resin to obtain S-adenosylmethionine refined solution; and d, salifying: concentrating the refined liquid obtained in the step c by 5-15 times through a nanofiltration membrane (the material is PVDF, and the interception molecular weight is 100-500D), and synthesizing the concentrated material and sulfuric acid and p-toluenesulfonic acid in a certain molar ratio to double salts so as to obtain the adenosylmethionine p-toluenesulfonic acid sulfate.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a method for extracting yeast after fermentation by using a traditional starch jet liquefaction device. Background technique [0002] Adenosylmethionine, also known as S-adenosylmethionine, its English name is S-Adenosyl-methionine (SAM), which is a combination of sulfur-containing amino acid-methionine (methionine) and the main energy substance of the human body, adenosine triphosphate (ATP-adenosinetriphosphate). The metabolite is a physiologically active substance that exists in all tissues and body fluids of the human body, participates in the body's transmethylation, polyamine synthesis and transsulfurization, and is effective for arthritis, depression, liver dysfunction, pancreatitis, etc. All have good curative effect. In 1999, the U.S. FDA approved S-adenosylmethionine as a health product, and quickly became one of the best-selling nutritional products in the Unit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H19/167C07H1/06
CPCC07B2200/07C07H1/06C07H19/167
Inventor 刘建民任明孙荣张雷
Owner 山东惠仕莱生物科技有限公司