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Method for preparing astaxanthin from haematococcus pluvialis

A technology of Haematococcus pluvialis and astaxanthin, applied in the direction of organic chemistry, can solve the problems of easy damage to molecular structure, structure damage, easy oxidation, etc., and achieve the effects of short extraction time, reduced damage, and short production cycle

Active Publication Date: 2019-11-12
ZHEJIANG LIZIYUAN FOOD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, due to the structure of astaxanthin itself, it is extremely sensitive to light, heat, and oxygen. It is easily oxidized when placed in the air for a long time, and its molecular structure is easily destroyed by high temperature. It is easily affected by external factors during the preparation process. Structural damage, resulting in varying degrees of loss

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Step 1) Grind the dried Haematococcus pluvialis into powder, sieve to obtain uniform Haematococcus pluvialls powder, add ethyl acetate / ethanol (5:1) at a ratio of solid to liquid of 1:100, and use a power of 300W microwave-assisted extraction for 8 minutes, rotary steaming to dryness, and astaxanthin crude extract;

[0028]Step 2) Put template molecule astaxanthin standard substance and functional monomer vinylpyridine into a beaker at a molar ratio of 1:8, shake at room temperature for 6 hours and dissolve in acetonitrile, add an appropriate amount of cross-linking agent EDMA and initiator ABIN, bubbling with N2 for 15 minutes after ultrasonication, and polymerizing in an oil bath at 60°C for 24 hours. Grind the obtained polymer, use methanol: acetic acid = 9:1 solution as eluent to remove template molecules, put it in a vacuum drying oven and dry to constant weight, and the obtained polymer is astaxanthin molecularly imprinted polymer.

[0029] Step 3) Put 50mg molec...

Embodiment 2

[0031] Step 1) Grind the dried Haematococcus pluvialis into powder, sieve to obtain uniform Haematococcus pluvialls powder, add ethyl acetate / ethanol (4:1) at a solid-liquid ratio of 1:300, and 360W microwave-assisted extraction for 6 minutes, rotary steaming to dryness, and astaxanthin crude extract;

[0032] Step 2) Put template molecule astaxanthin standard substance and functional monomer vinylpyridine into a beaker at a molar ratio of 1:4, shake at room temperature for 6 hours and dissolve in acetonitrile, add cross-linking agent EDMA and initiator ABIN, After ultrasonication, N2 was bubbled for 15 minutes, and polymerized in an oil bath at 60°C for 24 hours. Grind the obtained polymer, use methanol: acetic acid = 9:1 solution as eluent to remove template molecules, put it in a vacuum drying oven and dry to constant weight, and the obtained polymer is astaxanthin molecularly imprinted polymer.

[0033] Step 3) Put 50mg of molecularly imprinted polymer and 5ml of methanol...

Embodiment 3

[0035] Step 1) Grind the dried Haematococcus pluvialis into powder, sieve to obtain uniform Haematococcus pluvialls powder, add ethyl acetate / ethanol (4:1 ), with microwave-assisted extraction at a power of 480W for 7 minutes, and rotary steamed to dryness to obtain crude astaxanthin extract;

[0036] Step 2) Put template molecule astaxanthin standard substance and functional monomer vinylpyridine into a beaker at a molar ratio of 1:4, shake at room temperature for 6 hours and dissolve in acetonitrile, add cross-linking agent EDMA and initiator ABIN, After ultrasonication, N2 was bubbled for 15 minutes, and polymerized in an oil bath at 60°C for 24 hours. Grinding the obtained polymer, using a solution of methanol:acetic acid=9:1 as an eluent, removing template molecules, and drying in a vacuum drying oven to constant weight, the obtained polymer is an astaxanthin molecularly imprinted polymer;

[0037] Step 3) Put 50mg molecularly imprinted polymer and 5ml methanol solution ...

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PUM

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Abstract

The invention aims to provide a method for preparing astaxanthin from haematococcus pluvialis. The astaxanthin in the haematococcus pluvialis can be quickly and efficiently identified, the productiontime is greatly shortened, mechanical stability and thermal recessiveness are realized, the process is simple, operation is convenient, and industrial production is easy to realize.

Description

technical field [0001] The invention relates to a method for preparing astaxanthin from Haematococcus pluvialis by microwave-assisted combined molecular imprinting polymer method. Background technique [0002] Astaxanthin is a kind of ketone carotenoid, its molecular structure is 3, 3'-dihydroxy-4, 4'-diketone-β, β'-carotene, and its molecular formula is C 40 h 52 o 4 , widely exists in organisms such as shrimp, crab, microalgae and bird feathers, among which Haematococcus pluvialis ( Haematococcus pluvialis ) is a single-celled green algae with the highest natural astaxanthin content. Astaxanthin is a red lens, which contains conjugated double bonds, and has unsaturated ketone groups and hydroxyl groups at the end of the molecule, so the electronic effect is relatively active, and it can scavenge rational free radicals and produce strong antioxidant effects. According to research, astaxanthin is the only carotenoid that can pass through the blood-brain barrier, and its ...

Claims

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Application Information

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IPC IPC(8): C07C403/24
CPCC07C403/24C07C2601/16
Inventor 王顺余王平江瑜郑宋友何建新
Owner ZHEJIANG LIZIYUAN FOOD CO LTD
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