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An antibacterial, adhesive, conductive, hemostatic and antioxidative injectable composite hydrogel and its preparation method and application

A composite hydrogel and anti-oxidation technology, which can be used in pharmaceutical formulations, bandages, drug delivery, etc., and can solve problems such as limited applications and difficult dispersion of CNTs

Active Publication Date: 2020-10-27
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the strong hydrophobic interactions between individual CNTs make it difficult for unmodified CNTs to disperse in water
thus limiting its application in the synthesis of conductive bioscaffolds

Method used

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  • An antibacterial, adhesive, conductive, hemostatic and antioxidative injectable composite hydrogel and its preparation method and application
  • An antibacterial, adhesive, conductive, hemostatic and antioxidative injectable composite hydrogel and its preparation method and application
  • An antibacterial, adhesive, conductive, hemostatic and antioxidative injectable composite hydrogel and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0050] The preparation steps of gelatin grafted dopamine comprise:

[0051] (1A) Dissolve gelatin in a buffer solution, stir at 50-60°C for 10-30 minutes to fully dissolve, wherein the concentration of gelatin is 3-6 wt%, and the buffer solution can be phosphate buffered saline (PBS) and 2-morpholine Any one of the ethanesulfonic acid (MES) buffer solution, the pH of the buffer solution is 5-6;

[0052] (1B) Add dopamine hydrochloride to the solution obtained in step (1A), and stir at room temperature for 10-30 minutes to fully dissolve it, wherein the concentration of dopamine hydrochloride is 44-88 mg / mL;

[0053] (1C) Add EDC and NHS to the solution obtained in step (1B), and continue stirring at 30-50° C. for 6-18 hours, wherein the concentrations of EDC and NHS are 44-89 mg / mL and 27-54 mg / mL respectively; add EDC and After NHS, hydrochloric acid (1M) and aqueous sodium hydroxide solution (1M) should be used to maintain the reaction pH at 5-6.

[0054] (1D) After the st...

Embodiment 1

[0067] (1) Grafting dopamine to gelatin under the action of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) Gelatin-grafted dopamine (GT-DA) is generated on the main chain:

[0068] Gelatin (0.5 g, type A from pig skin, Sigma-Aldrich) was dissolved in 10 mL of phosphate buffered saline (PBS, pH=5.0) with stirring at 50°C. Then dopamine hydrochloride (0.741 g, Sigma-Aldrich) was added thereto, and stirred at room temperature for 30 min to fully dissolve it. Additional EDC (0.749 g, Sigma-Aldrich) and NHS (0.45 g, Sigma-Aldrich) were added. Stirring was continued for 12 hours at 37°C. Finally, the above reaction solution was dialyzed in deionized water for 48 hours, and then freeze-dried to obtain gelatin-grafted-dopamine. After adding EDC and NHS, and during dialysis with deionized water, the pH was maintained at 5 using hydrochloric acid (1M) and aqueous sodium hydroxide (1M).

[0069] (2) Preparation of polydopamine (PDA)-c...

Embodiment 2

[0076] The difference from Example 1 is that 1 wt% CNT-PDA in step (3B) of step (3) is replaced by 2 wt%, and the obtained hydrogel is also named GT-DA / CS / CNT2.

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Abstract

The invention relates to an injectable compound hydrogel with the functions of bacterial resistance, adhesion, electric conduction, bleeding stopping and oxidation resistance and a preparing method and application thereof. Firstly, dopamine is grafted on a gelatin main chain to generate gelatin grafted dopamine GT-DA; a polydopamine coating is prepared on the surface of CNT through auto-polymerization of the dopamine under an alkaline condition to obtain CNT-PDA; the CT-DA and CS are prepared into a GT-DA / CS solution, and the CNT-PDA is prepared into a CNT-PDA dispersion liquid; under the effect of an initiator, through the auto-polymerization of the dopamine in the GT-DA / CS solution and the CNT-PDA dispersion liquid, the injectable compound hydrogel with the functions of bacterial resistance, adhesion, electric conduction, bleeding stopping and oxidation resistance is obtained. The hydrogel prepared by grafting the dopamine on the gelatin main chain has good oxidation resistance, andalso has properties of good biocompatibility, adhesion, blood stopping, photo-thermal bacterial resistance, sustained drug release and the like.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to an injectable composite hydrogel with antibacterial adhesion, conductive conduction, hemostasis and anti-oxidation, its preparation method and application. Background technique [0002] Regeneration of damaged tissue is the most basic requirement for rebuilding tissue integrity and restoring normal tissue function. Infection is well known to be one of the major obstacles to wound healing and has become a growing factor in the cause of death in critically ill patients. The treatment of infection has brought a huge burden to the medical system and even the whole society. At present, the main strategy to deal with infection clinically is still to use antibiotics. Therefore, it is still challenging to develop an antibacterial drug delivery system that can not only effectively and accurately deliver antibiotics to wound sites, but also regulate the release of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L26/00
CPCA61L26/0004A61L26/0019A61L26/0023A61L26/0038A61L26/0061A61L26/0066A61L26/008A61L26/009A61L2300/406A61L2300/412A61L2300/602A61L2400/04A61L2400/12C08L79/02C08L5/08
Inventor 郭保林梁永平史梦婷赵鑫
Owner XI AN JIAOTONG UNIV