Quantum dot luminescence encoding microsphere with precisely designable encoding signals

Abstract

The invention discloses a quantum dot luminescence encoding microsphere with precisely designable encoding signals. The microsphere comprises a polymer and quantum dots with a large Stokes shift, which is greater than 50 nm. The emission wavelength of the quantum dots is 450 to 750 nm. The invention also discloses an application of the microsphere in detection of one or more target substances. Thespectrums of quantum dots with different emission wavelengths will not overlap with each other, thus energy transfer in multi-color encoding is avoided; the quantum dot luminescence encoding microsphere, which has precisely designable encoding signals, a strong encoding performance, and a large encoding number, is prepared; solved are the problems that the encoding signals cannot be predicted dueto energy transfer in multi-color encoding, the encoding signals cannot be precisely designed, and the encoding number is limited; the preparation of the encoding microsphere is not strongly dependent on experience anymore; the technology barrier and preparation cost of encoded library construction are largely reduced; and the microsphere has a very important meaning and wide application prospectin the field of liquid biochip detection.

Description

technical field [0001] The invention relates to the field of preparation and application of micro-nano materials, in particular to a quantum dot fluorescent coding microsphere whose coding signal can be precisely designed. Background technique [0002] The liquid phase chip is also called the suspension biochip. Its basic detection principle is to combine microspheres with flow cytometry, and use suspension microspheres as the reaction carrier for liquid phase detection. According to the interaction rules of protein-protein, nucleic acid-nucleic acid, etc. , with high-throughput, rapid detection flow cytometry as the analysis method. In the flow chamber of the flow cytometer, the microspheres are irradiated with laser light. On the one hand, the laser light is used to excite the fluorescence of the microspheres to identify the type of microspheres, and then determine the type of analyte; The fluorescent reporter molecule bound to the substance is detected, and the analyte i...

AI Technical Summary

Problems solved by technology

At present, some researchers control the placement of different luminescent materials in different microspheres, and then compound microspheres of different colors to eliminate energy transfer, which will undoubtedly complicate the preparation process of encoded microspheres

However, there is no report on solving energy transfer by controlling the spectral overlap of luminescent materials.

Images