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Cultivation method for increasing content of ganoderma lucidum polysaccharide and ganoderma lucidum total triterpenic acid

A cultivation method and technology of Ganoderma lucidum polysaccharide, applied in the cultivation field of Ganoderma lucidum, can solve the problems of long production cycle of fruit body cultivation, low content of biologically active substances, hindering mass production of Ganoderma lucidum, etc., and achieves widening raw material sources, small investment, and reduction of miscellaneous bacteria. The effect of pollution rate

Pending Publication Date: 2019-11-22
BEIJING JINGCHENG BIOTECHNOLOGY CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The fruiting bodies (or sclerotia) produced by traditional wild collection or artificial cultivation are scarce in wild resources, the fruiting body cultivation and production cycle is long (4-5 months), production is controlled by climate and other conditions, and the content of biologically active substances is low and many other factors Restrictions have hindered the mass production of Ganoderma lucidum

Method used

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  • Cultivation method for increasing content of ganoderma lucidum polysaccharide and ganoderma lucidum total triterpenic acid
  • Cultivation method for increasing content of ganoderma lucidum polysaccharide and ganoderma lucidum total triterpenic acid

Examples

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Effect test

Embodiment 1

[0046] A cultivation method of Ganoderma lucidum, comprising the following steps:

[0047] 1) Inoculate Ganoderma lucidum (Leyss.: Fr.) Karst parent species into the parent species medium and culture at a constant temperature of 22-26°C for 5-7 days to obtain the parent species of Ganoderma lucidum. The mother seed medium includes the following components: 250g / L potato, 25g / L glucose, 3g / L beef extract, 6g / L peptone, and 15-20g / L agar powder.

[0048] 2) Inoculate the parent ganoderma seed into the original seed medium and cultivate it at a temperature of 20-22° C. and a humidity of 55-65% to obtain the original ganoderma seed. The original seed medium includes the following components by weight: 70 parts of sawdust, 6 parts of corncobs, 15 parts of wheat bran, 2 parts of brown sugar, 1 part of gypsum powder, KNO 3 0.25 parts; the water content of the original seed culture medium is 55-60%; the original seed culture medium is made into a 500mL original seed bottle. The pre...

Embodiment 2

[0056] A cultivation method of Ganoderma lucidum, the only difference from Example 1 is that the mother seed culture medium includes the following components: 200g / L of potato, 20g / L of glucose, 3g / L of beef extract, 4g / L of peptone, 15g of agar powder / L; the original seed medium includes the following components by weight: 60 parts of sawdust, 5 parts of corncobs, 10 parts of wheat bran, 1 part of brown sugar, 1 part of gypsum powder, KNO 3 0.05 parts; the water content of the original seed medium is 55-60%; the cultivar medium includes the following raw materials in parts by weight: 50 parts of corn stalks, 30 parts of sawdust, 10 parts of pig manure, 10 parts of cow dung, rapeseed 5 parts of meal, 5 parts of humic acid, 1 part of Armillaria fermented liquid, 1 part of Bacillus subtilis fermented liquid, 1 part of gypsum; the effective number of viable bacteria in the Armillaria fermented liquid is 1.0-2.0×10 6 / mL, the number of effective viable bacteria in the Bacillus s...

Embodiment 3

[0059] A cultivation method of Ganoderma lucidum, the only difference from Example 1 is: the Ganoderma lucidum is Ganodermasinense J.D.Zhao, L.W.Hsu et X.Q.Zhang. The average daily growth rate of mother species, original species, cultivated species, and cultivation bag mycelium can reach 18-20mm / d. It generally takes 30-45 days from primordia to fruiting body maturity, and can be harvested 1.5-2 months after cultivation; based on the dry weight of 1000kg of dry substitute material, 90-115kg of Ganoderma lucidum fruiting bodies can be harvested in the same year.

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Abstract

The invention belongs to the technical field of artificial cultivation of medicinal fungi and particularly relates to a cultivation method for increasing the content of ganoderma lucidum polysaccharide and ganoderma lucidum total triterpenic acid. The method comprises the steps of separately obtaining ganoderma lucidum mother seeds, ganoderma lucidum original seeds and ganoderma lucidum culturingseeds; carrying out substitute cultivation on the ganoderma lucidum culturing seeds to obtain ganoderma lucidum, wherein a culture medium of the culturing seeds comprises corn straw, sawdust, pig manure, cow manure, rapeseed meal, humic acid, a fermented armillaria mellea liquor, a fermented bacillus subtilis liquor and gypsum, and a culture medium for substitute cultivation is the same as the culture medium of the culturing seeds. The method can remarkably increase the yield of ganoderma lucidum sporocarps and spore powder and the content of effective components such as the ganoderma lucidumpolysaccharide and the ganoderma lucidum total triterpenic acid.

Description

[0001] This application is a divisional application for an invention patent application with an application date of 2016.10.14, application number 201610900215.8, and the invention title "a method for cultivating Ganoderma lucidum". technical field [0002] The invention belongs to the technical field of artificial cultivation of medicinal fungi, and in particular relates to a cultivation method of ganoderma lucidum. Background technique [0003] Ganoderma lucidum (Ganoderma Lucidum Karst) is the fruiting body of the polyporaceae fungus Ganoderma lucidum. It has the effects of invigorating qi and calming the nerves, relieving cough and asthma, and prolonging life. For dizziness and insomnia, palpitation, shortness of breath, neurasthenia, fatigue and cough. Ganoderma lucidum is a kind of precious Chinese medicine in the treasure house of Chinese medicine, and it is an edible fungus integrating health care and medicine. "Shen Nong's Materia Medica", "Baopuzi", "Compendium o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/00A01G18/40A01G18/20
CPCA01G18/00A01G18/20A01G18/40Y02W30/40
Inventor 马传贵
Owner BEIJING JINGCHENG BIOTECHNOLOGY CO LTD
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