Platelet-rich plasma tissue engineering scaffold and preparation method thereof

A tissue engineering scaffold and bone scaffold technology, applied in the field of biomedicine, can solve the problems of not effectively promoting rapid and high-quality healing of patients, poor recovery of motor function, unsatisfactory healing effect, etc., and achieve high-quality rapid healing , excellent sustained release effect, excellent sustained release and accelerated healing effect

Inactive Publication Date: 2019-11-22
XIANGYA HOSPITAL CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, after tendon-bone interface injury, non-steroidal anti-inflammatory drugs, cryotherapy, low-intensity pulsed ultrasound, physical therapy, etc. are often used clinically. Although these treatments are widely used in the field of orthopedics or sports medicine, early motor function of patients still recovers. Poor, its healing effect is unsatisfactory, and cannot effectively promote rapid and high-quality healing of patients

Method used

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  • Platelet-rich plasma tissue engineering scaffold and preparation method thereof
  • Platelet-rich plasma tissue engineering scaffold and preparation method thereof
  • Platelet-rich plasma tissue engineering scaffold and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Embodiment 1 PRP-loaded tissue engineering scaffold of icariin and preparation method thereof (1)

[0057] 1. Preparation of concentrated PRP

[0058] 20 mL of autologous blood was taken from the rabbit's ear vein, and the red blood cells were removed by gradient centrifugation (differential speed centrifugation), firstly at 300 g x 10 min. Then 800g×10min, take 2.4mL of the lower layer, and discard the remaining upper layer liquid; shake well and shake for 30s to prepare concentrated PRP.

[0059] 2. Preparation of PRP-loaded icariin tissue engineering scaffold

[0060] Dissolve icariin in DMSO to prepare a DMSO solution (ICA-DMSO solution) containing 50 mM icariin, take 10 μL ICA-DMSO solution and 0.1 mL of mass concentration 10% calcium chloride solution (solvent is 0.1M PBS solution) and 1.0 mL of concentrated PRP were mixed to obtain a PRP-loaded icariin gel. After lyophilization in a vacuum freeze dryer and ethylene oxide gas sterilization, the tissue engineeri...

Embodiment 3

[0068] Example 3 Test of Icariin Sustained Release Effect in Tissue Engineering Scaffold

[0069] The stents prepared in Example 1 and Comparative Example 3 were immersed in 1 mL of sterile PBS solution in the centrifuge tube at room temperature, and kept stirring gently. At the time points of 4d, 5d, 6d, and 7d, take 0.6 mL of incubation solution each, and add a new equal volume of PBS liquid to the centrifuge tube at the same time after the liquid. Use high-performance liquid chromatography (HPLC) to detect the corresponding peak area of ​​ICA in the sample to be tested, and calculate the corresponding ICA concentration in the sample to be tested according to the standard curve, thereby quantifying the in vitro release curve of icariin , the results are shown in Table 1, 2 and figure 2 shown.

[0070] Table 1 Cumulative release rate of ICA in PRP loaded ICA scaffold (%)

[0071] Bracket category 0h 1h 2h 4h 8h 16h 1d 2d 3d 4d 5d 6d 7d freeze-d...

Embodiment 5

[0075] Example 5 Tissue Engineering Scaffold Treatment Effect Test

[0076] 1. Establishment and grouping of animal models

[0077] Select 48 healthy adult New Zealand white rabbits, male or female, body weight (3.0±0.5) kg, rabbits fed with common feed, 24h cycle light, free to eat and drink, all animals have no trauma, infection and deformity in the operation area, and no For other systemic diseases, all operating procedures complied with the guidelines for animal experiments.

[0078] 48 white rabbits were randomly divided into 3 groups according to the commonly used grouping method in animal experiment scientific research design (random number table method), 16 in each group. They were patellar injury group (control group-CLT); freeze-dried PRP scaffold group (FD-PRP); freeze-dried PRP-loaded ICA scaffold group (FD-PRP / ICA).

[0079] Animals were anesthetized with Sumianxin, disinfected, and draped. An incision was made on the medial side of the patellar tendon of the ri...

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Abstract

The invention belongs to the field of biological medicine, and relates to a composite biological material of platelet-rich plasma tissue and a preparation method thereof. The composite biological material comprises PRP loaded with icariin, has the functional characteristics of good biological activity, chondrogenic property, osteogenic property and bone healing property, and can be used as a noveltissue engineering scaffold for bone tendon interface damage, is used for treating injury of a bone tendon interface, and realizes high-quality rapid healing of the bone tendon interface, the composite biological material shows the excellent slow-release effect, and can be slowly released at a proper speed for a proper duration, so that the drug effect of icariin is exerted to a greater extent, and side effects caused by over-high dosage are avoided.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to a platelet-rich plasma tissue engineering scaffold and a preparation method thereof. Background technique [0002] The tendon-bone interface is a common type of sports injury in the field of sports medicine. According to statistics, in the United States alone, there are about 250,000 rotator cuff injuries and 100,000 anterior cruciate ligament injuries requiring surgical treatment each year, which has brought a heavy economic burden to society and families. With the increase in life expectancy and the widespread development of national fitness campaigns, the incidence of tendon-bone interface injuries is expected to continue to rise. [0003] After the tendon-bone interface is injured, the tendon or ligament is often directly fixed to the bone by means of various surgical suture techniques. The tendon-bone interface is filled with fibrous scar tissue in the early stage, and the reconstr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/02A61L27/50A61L27/54
CPCA61L27/025A61L27/3616A61L27/50A61L27/54A61L2300/236A61L2300/412A61L2300/602A61L2430/02
Inventor 陈灿史强陈洋
Owner XIANGYA HOSPITAL CENT SOUTH UNIV
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