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Penicillium oxalicum capable of degrading 2-phenethyl alcohol produced from rice straw and application thereof

A technology of Penicillium oxalicum and phenylethyl alcohol, applied in the field of microorganisms, can solve the problems of no Penicillium oxalicum and no Penicillium oxalicum fermenting natural straw, and achieve good cellulose degradation ability and strong endoglucosidase activity Effect

Active Publication Date: 2019-11-22
INST OF MICROBIOLOGY JIANGXI ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at present, there is no report of Penicillium oxalicum fermentation to produce 2-phenylethanol, let alone the report of Penicillium oxalicum fermentation of natural rice straw to produce 2-phenylethanol

Method used

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  • Penicillium oxalicum capable of degrading 2-phenethyl alcohol produced from rice straw and application thereof
  • Penicillium oxalicum capable of degrading 2-phenethyl alcohol produced from rice straw and application thereof
  • Penicillium oxalicum capable of degrading 2-phenethyl alcohol produced from rice straw and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0034] The screening of embodiment 1 degrading cellulose strain

[0035] Screening medium: CMC-Na 10g, (NH 4 ) 2 SO 4 1.4g, MgSO 4 0.3g, KH 2 PO 4 2g, MnSO 4 1.6mg, FeSO 4 5mg, ZnSO 4 2.5mg, CoCl 2 2.0mg, 20g agar, pH=7.0, dilute to 1000mL;

[0036] Seed medium: CMC-Na 10g, peptone 3g, KH 2 PO 4 4g, MgSO 4 ·7H 2 O 0.03g, pH=6.0, dilute to 1000mL;

[0037] Fermentation enzyme production medium: CMC-Na 10g, (NH 4 ) 2 SO 4 4.0g, MgSO 4 ·7H 2 O 0.5g, K 2 HPO 4 2g, 5g of beef extract, 10g of peptone, natural pH, dilute to 1000mL;

[0038] Preliminary screening: Weigh 10g of soil sample, put it into a conical flask filled with 90mL of sterile water, place it on a shaker at 150rpm for 30min, take it out, and dilute it step by step to 10 -1 , 10 -2 , 10 -3 , 10 -4 , 10 -5 , 10 -6 6 kinds of concentrations, the dilutions of 6 kinds of concentrations were spread on the screening medium, repeated 3 times, and cultured at 25°C for 3 days. After the hypha...

Embodiment 2

[0041] Degradative enzyme activity assay of embodiment 2 degrading cellulose strain

[0042] 3,5-Dinitrosalicylic acid colorimetric method (DNS method) was used to measure filter paper enzyme activity, endo-glucosidase activity, exo-glucosidase activity and β-glucosidase activity.

[0043] 2.1 Preparation of glucose standard curve

[0044] Glucose standard solution: weigh 1 g of anhydrous glucose dried at 103°C to constant weight, and dilute to 100 mL with water;

[0045] Phosphate buffer: 0.1mol / L pH 6.0 (suitable for neutral cellulase) Weigh 121.0 g of sodium dihydrogen phosphate monohydrate and 21.89 g of disodium hydrogen phosphate dihydrate, and dissolve them in 1 L of deionized water. Adjust the pH of the solution to 6.0;

[0046] DNS reagent: Weigh 10g of 3,5-dinitrosalicylic acid, put it in about 600mL of water, gradually add 10g of sodium hydroxide, stir and dissolve in a 50°C water bath (magnetic force), then add 200g of potassium sodium tartrate in turn , 2 g of ...

Embodiment 3

[0080] The filter paper degradation assay of embodiment 3 degrading cellulose strain

[0081] Filter paper degradation medium: (NH 4 ) 2 SO 4 1.4g, MgSO 4 0.3g KH 2 PO 4 2g, MnSO 4 1.6mg, FeSO 4 5mg, ZnSO 4 2.5mg, CoCl 2 Add 2.0 mg of distilled water to 1 L, take 5 mL of culture base in a test tube, add 50 mg of filter paper, 121 ° C for 20 min. After sterilization, use an inoculation needle to pick up a small amount of T1 spores and inoculate them in a test tube, and incubate at 28°C.

[0082] The result is as Figure 4 As shown (the test tubes 1 to 3 on the left are the strain T1, and the test tube on the far right is the control), the strain T1 can disintegrate the filter paper, indicating that its filter paper enzyme activity is very high.

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Abstract

The invention provides penicillium oxalicum capable of degrading 2-phenethyl alcohol produced from rice straw and application thereof. The penicillium oxalicum is penicillium oxalicum T1, and was preserved in the China Center for Type Culture Collection. The preservation number is CCTCC M 2019608. The penicillium oxalicum T1 provided by the invention has good cellulose degradation ability, can produce cellulase, and has strong endoglucosidase activity, filter paper enzyme activity, exonuclease activity and beta-glucosidase activity; and the penicillium oxalate T1 can degrade 2-phenethyl alcohol produced by degradation of rice straw. The fermentation broth that degrades rice straw can inhibit Phomopsis sp., Magnaporthe grisea and Fusarium oxysporum.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a Penicillium oxalicum capable of degrading rice straw to produce 2-phenylethanol and an application thereof. Background technique [0002] The southern part of my country is dominated by rice planting and has abundant rice straw resources. Direct burning of rice straw as fuel is not only a huge waste of natural resources, but also causes serious environmental pollution. The lignocellulose in the straw component has a complex structure and degrades slowly under natural conditions. In industry, high temperature and high pressure or strong alkali are often used for pretreatment, but these measures not only consume energy but also cause environmental pollution. Therefore, the use of microorganisms to treat rice straw has become a focus of research in recent years, because it has the advantages of less investment, simple operation, and no environmental pollution compared...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P7/22C12N9/42C12R1/80
CPCC12P7/22C12N9/2437C12Y302/01004C12Y302/01091C12Y302/01021C12R2001/80C12N1/145
Inventor 王金昌靳亮吴磊黄筱萍刘兰
Owner INST OF MICROBIOLOGY JIANGXI ACADEMY OF SCI
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