Feed for hybrid fish and preparation method and application thereof
A fish feed and raw material technology, applied in the field of hybrid fish feed and its preparation, can solve the problems of slowing down of fish growth, weight loss, and increasing the amount of ammonia nitrogen in the breeding system, etc.
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Embodiment 1
[0077] Example 1: Crossbreeding of bream and bream and construction of backcross offspring
[0078] 1 experimental fish
[0079] The female parent used in the experiment is Megalobrama amblycephala (MA) "Pujiang No. 1" fine variety (approval unit: National Aquatic Raw Variety Approval Committee, approval number: GS-01-001-2000); the male parent is Dianshanhuqiao The original species of Culter alburnus (CA) (taken from the Shanghai Dianyuan Aquatic Breeding Farm) has a slender body, flat sides, in the shape of willow leaves, a straight back of the head, and a raised back of the head. The parents are 3-year-old, sexually mature and well-developed, and they are both kept in the Genetic Breeding Center of Bory Bream, Ministry of Agriculture, Shanghai Ocean University.
[0080] 2 experimental reagents
[0081] Chorionic gonadotropin, luteinizing hormone releasing hormone, colchicine, DAPI (Germany Partec), PHA (Shanghai Sunshine), NaCl, KCl, disodium hydrogen phosphate (12H 2 O)...
Embodiment 2
[0085] Example 2: Research on feed for offspring of crossbreeding and backcrossing bream and bream
[0086] 1 Experimental design and feed formulation
[0087] In this study, the dietary protein level was taken as the influencing factor, and other factors were controlled relatively constant. Among them, the five levels of protein are 25%, 30%, 35%, 40% and 45%, respectively. The animal protein source of feed preparation is Peruvian fish meal (TASA super steam red fish meal), and the plant protein source is soybean meal and rapeseed meal (purchased from Shanghai Dongchen Grain and Oil Co., Ltd.). Soybean oil was used as the fat source, and the fat level of the feed was fixed at about 4.5%. Sugars are provided by wheat flour and wheat bran to balance the level of nitrogen-free extract. α-starch is used as the binder, effective phosphorus is adjusted with calcium dihydrogen phosphate; vitamins and mineral salts are supplemented with premixed materials. The experimental feed f...
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