Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of β-galactose modified nitric oxide sustained-release hydrogel and its preparation method and application

A technology of nitric oxide and galactose, which is applied in the field of biomedical engineering, can solve problems such as applications that have not yet been reported, and achieve the effects of excellent physical and chemical properties, clear polymer structure, and easy structure.

Active Publication Date: 2021-06-11
JINAN UNIVERSITY
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, polyethylene glycol-modified PAMAM (MPEG-PAMAM) guest molecules have been used to load NO through the secondary amine groups of the PAMAM segment to form ionic donors, which are then modified with β-galactose (Gal) , in order to obtain a novel NO ion donor PEG-PAMAM-NO-Gal that exists stably in water, and then form a NO-loaded injectable hydrogel material and its application in aqueous solution through the host-guest assembly with α-CD to report

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of β-galactose modified nitric oxide sustained-release hydrogel and its preparation method and application
  • A kind of β-galactose modified nitric oxide sustained-release hydrogel and its preparation method and application
  • A kind of β-galactose modified nitric oxide sustained-release hydrogel and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] (1) Synthesis of PAMAM (PAMAM-NO) loaded with nitric oxide

[0065] Synthesis of Polyamide Dendrimers Containing Alkyne Groups (PAMAM)

[0066] Referring to the preparation method of Example 4 in the patent (Application No. 201610356643.9, a cationic polymer loaded with nitric oxide and its preparation method and application), an alkyne-containing polyamide dendrimer was prepared.

[0067] PAMAM and CH 3 ONa dissolved in CH 3 OH, PAMAM and CH 3 The molar ratio of ONa is 1:200; CH 3 The dosage of OH is to dissolve 0.1g CH per 10mL 3 ONa meter, after stabilizing for 10 minutes, place it in a high-pressure reactor and seal it, feed high-purity nitrogen for 5 minutes and maintain the pressure of the reactor at 10 psi, remove the air in the reactor, then feed NO gas (40 psi), and react at room temperature for 3 sky. After the reaction is over, feed high-purity nitrogen gas (10psi) for 10 minutes to discharge unreacted NO, open the reaction vessel, and take out the reac...

Embodiment 2

[0077] (1) Synthesis of PAMAM (PAMAM-NO) loaded with nitric oxide

[0078]Synthesis of Polyamide Dendrimers Containing Alkyne Groups (PAMAM)

[0079] Referring to the preparation method of Example 5 in the patent (Application No. 201610356643.9, a cationic polymer loaded with nitric oxide and its preparation method and application), an alkyne-containing polyamide dendrimer was prepared.

[0080] PAMAM and CH 3 ONa dissolved in CH 3 OH, PAMAM and CH 3 The molar ratio of ONa is 1:300; CH 3 The dosage of OH is to dissolve 0.3g CH per 10mL 3 ONa meter, stabilized for 30 minutes, placed in a high-pressure reactor and sealed, fed high-purity nitrogen for 15 minutes and maintained the pressure of the reactor at 20 psi, removed the air in the reactor, and then fed NO gas (80 psi), and reacted at room temperature for 7 days . After the reaction is over, feed high-purity nitrogen gas (20psi) for 20 minutes to discharge unreacted NO, open the reactor, and take out the reaction prod...

Embodiment 3

[0090] (1) Synthesis of PAMAM (PAMAM-NO) loaded with nitric oxide

[0091] Synthesis of Polyamide Dendrimers Containing Alkyne Groups (PAMAM)

[0092] Referring to the preparation method of Example 6 in the patent (Application No. 201610356643.9, a cationic polymer loaded with nitric oxide and its preparation method and application), an alkyne-containing polyamide dendrimer was prepared.

[0093] PAMAM and CH 3 ONa dissolved in CH 3 OH, PAMAM and CH 3 The molar ratio of ONa is 1:500; CH 3 The dosage of OH is to dissolve 0.5g CH per 10mL 3 ONa meter, after stabilizing for 20min, place it in a high-pressure reactor and seal it, feed high-purity nitrogen for 10min and maintain the pressure of the reactor at 15psi, remove the air in the reactor, then feed NO gas (80psi), and react at room temperature for 5 sky. After the reaction is over, feed high-purity nitrogen gas (15psi) for 15 minutes to discharge unreacted NO, open the reaction vessel, and take out the reaction produc...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a β-galactose modified nitric oxide slow-release hydrogel, a preparation method and application thereof. The preparation method comprises the following steps: dissolving dendrimer PAMAM in CH 3 ONa / CH 3 After the OH solution is placed in a nitric oxide autoclave to load nitric oxide, the halogenated β-D-galactose pentaacetate is added to protect the nitric oxide active group, and then the mPEG is coupled by a click reaction to obtain mPEG-PAMAM- NO‑Gal; Dissolve mPEG‑PAMAM‑NO‑Gal in deionized water, mix with β‑CD aqueous solution, let stand, and gradually form a gel. The preparation method is mild, easy to operate, less by-products and easy to separate and purify the product, the obtained hydrogel has good biocompatibility, and its nitric oxide slow-release performance is widely used in biomedical engineering, especially in the field of antibacterial.

Description

technical field [0001] The invention belongs to the field of biomedical engineering, and in particular relates to a β-galactose modified nitric oxide slow-release hydrogel and a preparation method and application thereof. Background technique [0002] Periodontitis is one of the most common diseases in humans. Severe periodontitis leads to tooth loss and systemic health deficits such as coronary heart disease, cardiovascular disease, and stroke. Among the various microorganisms in the oral cavity, the overgrowth of periodontal pathogens (such as Porphyromonas gingivalis) leads to the occurrence and development of periodontitis. Long-term use of antibiotics is prone to drug resistance and destroys the micro-ecological balance of the oral cavity. Therefore, there is an urgent clinical need for a new type of antibacterial material that is safe, persistently antibacterial, can be administered locally, and is not prone to drug resistance. [0003] In recent years, it has been ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C08G81/00A61K47/69A61K47/59A61K47/60A61K9/06A61K33/00A61P1/02
CPCA61K9/0019A61K9/06A61K33/00A61K47/595A61K47/60A61K47/6903A61K47/6951A61P1/02C08G81/00
Inventor 马栋刘施欣张武薛巍
Owner JINAN UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com