Melanosome in vitro cell model and preparing method thereof

A cell model, melanin technology, applied in biochemical equipment and methods, animal cells, vertebrate cells, etc., can solve the problems of natural skin structure differences, poor stability, difficult to maintain, etc., and achieve good culture stability and good mechanical properties. Effect

Inactive Publication Date: 2019-12-10
JIANGYIN USUN BIOCHEMICAL TECH CO LTD
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  • Abstract
  • Description
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Problems solved by technology

[0007] The purpose of the present invention is to provide a method for preparing a melanin in vitro model, the prepared melanin model and its appli

Method used

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Embodiment 1

[0022] The cross-linked agarose microspheres were put into 0.01% sodium hyaluronate, soaked for 60 minutes, taken out, and dried at room temperature. Agarose microspheres containing sodium hyaluronate were placed in 0.05 ng / ml of fibroblast growth factor. Put the agarose microspheres loaded with fibroblast growth factor into 0.01 mg / ml collagen solution, soak for 30 minutes, add fibroblasts, the cell seeding density is 1*105 / cm2, culture with 10% 10*DMEM Cultured with fetal bovine serum, submerged at 37°C for 12 hours; repeated inoculation of fibroblasts 4 times, and cultured. Inoculate 1*107 / cm2 mast cells on its surface and culture for 24 hours. Repeat inoculation of fibroblasts 3 times.

[0023] Mix keratinocytes and melanocytes at a ratio of 1:5, inoculate the cells at a density of 2.5×106 / cm2, and carry out submerged culture. The medium is 10% 10*DMEM culture and fetal bovine serum culture, containing insulin 0.5 ng / ml, epidermal growth factor 0.1ng / ml, immersion cultu...

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Abstract

The invention relates to a method for preparing a melanosome in vitro model. The method comprises the following steps of using a peridium porous agarose microsphere as a cell matrix, and constructinga derma layer containing melanosome and an epidermal layer on the cell matrix. The constructed model is similar to the structure of the human body skin, and comprises a part of inflammation stress factors, so that screening of products relevant to melanosome metabolic diseases and researching of a melanosome formation or restraining mechanism are facilitated.

Description

technical field [0001] The invention relates to the field of biological materials, in particular to a melanin in vitro cell model and a preparation method thereof. Background technique [0002] Melanin, the main pigment that determines the color of human skin and hair, is synthesized and stored by melanocytes, which remain active in the skin throughout life. Disorders of melanin metabolism can cause skin pigmentation disorders, including hyperpigmentation such as spots, moles, and hypopigmentation such as white hair, albinism, and vitiligo. The onset of pigment metabolic diseases is relatively common, and there is an upward trend with the deterioration of the environment, and it is still an unresolved disease. Therefore, research on the pathogenesis of pigment metabolic diseases, therapeutic drugs, and therapeutic cosmetics has become a topic of concern. [0003] Products for the treatment of pigmentation metabolic diseases usually undergo a large number of tests before th...

Claims

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Application Information

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IPC IPC(8): C12N5/071
CPCC12N5/0698C12N2501/115C12N2502/091C12N2502/094C12N2502/11C12N2502/1323C12N2533/76C12N2533/80
Inventor 高敏
Owner JIANGYIN USUN BIOCHEMICAL TECH CO LTD
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