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Composition taking hederacolchiside A1 as active component and use of composition

A technology for hedera saponins and active ingredients, which is applied in the field of medicine, can solve the problems of difficulty in obtaining products with stable composition, controllable process, complicated methods, etc., and achieves controllable process and quality, simple and easy process, and simple and easy preparation method. row effect

Inactive Publication Date: 2019-12-13
SHENYANG PHARMA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The existing preparation technology of Hedera saponin A1 mostly adopts the method of first extracting and then hydrolyzing medicinal materials, which is relatively cumbersome. The present invention adopts the simultaneous parallel steps of hydrolysis and extraction, which greatly saves time and economic cost; on the other hand, the existing The reported technology is not sure about the composition of the saponin composition in Liangtoujian products, so it is difficult to obtain products with stable composition and good process controllability in actual production; , Paeoniae saponin B, Saponin Pe, Phylloxanthin R22 and Phytocyperone R23 have significant pharmacological activities, especially the biological activity of Hedera saponin A1 is particularly significant, so how to prepare rich and high-purity Composition of Black Sea Hedera saponin A1 appears particularly urgent

Method used

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  • Composition taking hederacolchiside A1 as active component and use of composition
  • Composition taking hederacolchiside A1 as active component and use of composition
  • Composition taking hederacolchiside A1 as active component and use of composition

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Experimental program
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Effect test

Embodiment 1

[0025] Embodiment 1 composition preparation method 1

[0026] Pulverize 1000g of anemone rhizome, pass through a 10-mesh sieve, add 8 times the amount of 0.2M 55% ethanol sodium hydroxide alkali solution, soak for 6 hours, reflux and extract three times (8 times, 8 times, 8 times the amount of alcohol added each time) ), 1.5 hours each time, combined the extraction solution, recovered until there was no alcohol smell, diluted the medicinal solution with distilled water, and adsorbed it with HPD-400 macroporous resin for 5 hours. The amount of resin used was 1kg. Elution to remove impurities, and then eluted with 12L 80% ethanol at a rate of 0.8L / h, collect the eluted part of 90% ethanol, spin dry, put on ODS column, elution with 5% ethanol to remove impurities, the elution process of this step is passed by TLC Monitoring, and then eluted with 95% ethanol, collected 95% ethanol eluted part, evaporated to dryness, obtained target product 320g, black sea hedera saponin A1 content...

Embodiment 2

[0027] Embodiment 2 composition preparation method 2

[0028] Pulverize 1000g rhizome of Anemone anemone, pass through a 16-mesh sieve, add 8 times the amount of 0.8M 65% ethanol strong potassium oxide solution, soak for 12 hours, reflux extraction three times (8 times, 6 times, 6 times), each 2h, Combine the extraction solutions, recover until there is no alcohol smell, and use AB-8 macroporous resin to absorb for 8 hours. The amount of resin used is 1.5kg. % ethanol was eluted at a rate of 0.6L / h, the eluted part of 80% ethanol was collected, spin-dried, put on ODS column, eluted with 10% ethanol to remove impurities, the elution process of this step was monitored by TLC, and then eluted with 90% ethanol , collect the 90% ethanol eluted part, evaporate to dryness, the obtained target product is 300g, the content of Hedera saponin A1 is 45.1%, acanthoside K, paeony saponin B, Saponin Pe, Phylloxanthin R22 and Phylophora saponin The summed content of the five components of pr...

Embodiment 3

[0029] Embodiment 3 composition preparation method 3

[0030] Pulverize 1000g rhizome of Anemone anemone, pass through a 16-mesh sieve, add 8 times the amount of 0.3M 80% ethanol strong potassium oxide solution, soak for 12 hours, reflux extraction three times (8 times, 8 times, 6 times), each 2h, Combine the extraction solutions, recover until there is no alcohol smell, and use HPD-400 macroporous resin to absorb for 4 hours. The amount of resin used is 1.5kg. Ethanol was eluted at a rate of 0.5L / h, and the eluted part with 80% ethanol was collected, spin-dried, put on an ODS column, and eluted with 25% ethanol to remove impurities. The elution process in this step was monitored by TLC, and then eluted with 85% ethanol. Collect 85% ethanol elution part, evaporate to dryness, the obtained target product 270g, black sea hedera saponin A1 content 52.6%, acanthoside K, paeony grass saponin B, Saponin Pe, bamboo cyperone R22 and bamboo cyperone The content of the five components ...

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PUM

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Abstract

The invention discloses a composition taking hederacolchiside A1 as a main component. The composition is characterized by mainly comprising the hederacolchiside A1, eleutheroside K, leonloside B, Saponin Pe, raddeanin R22 and raddeanin R23; the total mass content of the hederacolchiside A1, the eleutheroside K, the leontoside B, the Saponin Pe, the raddeanin R22 and the raddeanin R23 in the composition ranges from 60.00% to 100%. The composition can be used for preventing and treating rheumatic arthritis and inflammatory pain.

Description

technical field [0001] The invention relates to the technical field of medicine, and relates to a composition with Hedera saponin A1 as the main active ingredient, a preparation method and a medical application thereof. Background technique [0002] The two tips are the rhizomes of Anemone raddeana Regel, which are mainly produced in Northeast China, North China, Japan, Korea, and the Far East of Russia. This medicine was first recorded in "The Essence of Materia Medica" written by Liu Wentai in the Ming Dynasty. In the Qing Dynasty, Wang Xugao's "Ding'ai Powder" once used two tips to treat breast cancer. Now it is clinically used for anemofrigid-damp arthralgia, spasm of hands and feet, arthralgia, carbuncle swelling and pain. [0003] Since the 1980s, researchers have done a lot of research on the chemical composition, pharmacological effects and clinical application of Liangtujian, providing a lot of research information for the modern application of this traditional Chi...

Claims

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Application Information

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IPC IPC(8): A61K36/71A61K31/704A61P19/02A61P29/00
CPCA61K31/704A61K36/71A61K2236/333A61K2236/50A61P19/02A61P29/00
Inventor 路金才吕重宁张丹丹孙崇智
Owner SHENYANG PHARMA UNIVERSITY
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