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Medical dressing patch containing human fibroblast adjusting culture solution, and preparation method and application thereof

A technology of human fibroblasts and culture medium, applied in the field of medical devices, can solve the problems of ineffective effects, achieve the effects of no toxic side effects, promote cell migration, and reduce stimulation

Active Publication Date: 2019-12-13
西安艾尔菲生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The third object of the present invention is to provide the application of the above-mentioned medical dressing in hormone-dependent dermatitis, and solve the problem that the effect of existing dressing products is not obvious

Method used

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  • Medical dressing patch containing human fibroblast adjusting culture solution, and preparation method and application thereof
  • Medical dressing patch containing human fibroblast adjusting culture solution, and preparation method and application thereof
  • Medical dressing patch containing human fibroblast adjusting culture solution, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] A medical dressing containing human fibroblast-regulated culture fluid, the preparation method of which is as follows:

[0077] Step 1, the preparation of liposome freeze-dried powder A of encapsulating human fibroblasts to adjust the culture medium:

[0078] Step 1.1, human fibroblast-conditioned medium concentrated by ultrafiltration:

[0079] Concentrate the conditioned human fibroblast culture solution by ultrafiltration, and filter the filtrate through a 0.22 μm sterile filter to obtain a conditioned human fibroblast culture concentrated solution, the concentration of which is 10 mg / mL.

[0080] Step 1.2, preparation of liposome suspension wrapped in human fibroblast conditioned medium:

[0081] Dissolve 15.0g of soybean lecithin, 5.0g of cholesterol, and 1.0g of vitamin E in 63mL of absolute ethanol, and remove the absolute ethanol by rotary evaporation under reduced pressure at 0.04Mpa and 35°C to obtain a uniform film; take step 1.1 to obtain Add 20 mL of 10 m...

Embodiment 2

[0089] A medical dressing containing human fibroblast-regulated culture fluid, the preparation method of which is as follows:

[0090] Step 1, preparation of human fibroblast regulated culture medium liposome lyophilized powder A:

[0091]Step 1.1, human fibroblast-conditioned medium concentrated by ultrafiltration:

[0092] Concentrate the conditioned human fibroblast culture solution by ultrafiltration, and filter the filtrate through a 0.22 μm sterile filter to obtain a conditioned human fibroblast culture concentrated solution with a concentration of 15 mg / mL.

[0093] Step 1.2, preparation of liposome suspension wrapped in human fibroblast conditioned medium:

[0094] Dissolve 12g of soybean lecithin, 4g of cholesterol, and 0.5g of vitamin E in 64mL of absolute ethanol, and remove the absolute ethanol by rotary evaporation under reduced pressure at 0.05Mpa and 37°C to obtain a uniform film; take 15mg prepared in step 1.1 / mL human fibroblast adjusted culture concentrate...

Embodiment 3

[0102] A medical dressing containing human fibroblast-regulated culture fluid, the preparation method of which is as follows:

[0103] Step 1, preparation of human fibroblast regulated culture medium liposome lyophilized powder A:

[0104] Step 1.1, human fibroblast-conditioned medium concentrated by ultrafiltration:

[0105] Concentrate the conditioned human fibroblast culture solution by ultrafiltration, and filter the filtrate through a 0.22 μm sterile filter to obtain a conditioned human fibroblast culture concentrated solution, the concentration of which is 20 mg / mL.

[0106] Step 1.2, preparation of liposome suspension wrapped in human fibroblast conditioned medium:

[0107] Dissolve 3.0g of soybean lecithin, 1.0g of cholesterol, and 3.0g of vitamin E in 35ml of absolute ethanol, and remove the absolute ethanol by rotary evaporation under reduced pressure at 0.06Mpa and 40°C to obtain a uniform film; take step 1.1 to obtain Add 25mL of 20mg / mL human fibroblast conditio...

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Abstract

The invention discloses a medical dressing patch containing a human fibroblast adjusting culture solution; a feed liquid part is composed of the following components by the mass percentage: 1-8% of liposome freeze-dried powder A wrapped with the human fibroblast adjusting culture solution and 92-99% of a mixed solution B, wherein the sum of the mass percentages of the components is 100%, and the component A and the component B are independently packaged. According to the medical dressing patch, the liposome freeze-dried powder wrapped with the human fibroblast adjusting culture solution is combined with the mixed solution, the medical dressing patch is applied to wound repair, absorption of active ingredients in the culture solution is facilitated, the transdermal absorption capacity of cytokines and collagen is enhanced, and the bioavailability of the cytokines in the human fibroblast adjusting culture solution is improved. Besides, sodium hyaluronate can promote cell migration, differentiation and wound healing and has high-viscosity elasticity, plasticity and good biocompatibility, and repair of skin injury parts is facilitated by combining sodium hyaluronate and freeze-dried powder.

Description

technical field [0001] The invention belongs to the technical field of medical devices, and in particular relates to a medical dressing containing human fibroblast regulated culture solution, the invention also relates to a preparation method of the medical dressing, and the application of the above medical dressing. Background technique [0002] With the development of our country's society and economy, the medical device market is growing day by day, and the repair of facial trauma has become a topic that people are more and more concerned about. Various hyaluronic acid and collagen-based medical dressings are constantly being used in facial beauty and trauma treatment. . The process of wound repair is not only the process of cell proliferation, differentiation, migration, apoptosis, and disappearance, but also the result of a series of network interactions between different types of cells, structural proteins, and growth factors. Studies have confirmed that growth factor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/40A61L15/20A61L15/28A61L15/44
CPCA61L15/40A61L15/20A61L15/28A61L15/44A61L2300/30A61L2300/412
Inventor 肖萍王海江王静胡春梅
Owner 西安艾尔菲生物科技有限公司
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