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Human fibroblast regulating culture liquid flexible liposome, preparation method and application thereof

A human fibroblast, flexible liposome technology, applied in the field of biomedical beauty and skin care, can solve problems such as poor compatibility and insignificant direct application effect, achieve uniform particle size, solve difficult transdermal absorption, and improve bioavailability degree of effect

Active Publication Date: 2019-11-15
西安艾尔菲生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The third object of the present invention is to provide the above-mentioned human fibroblast regulated culture solution flexible liposome application in the technical field of beauty and skin care, so as to solve the problem that the direct application effect of the existing human fibroblast regulated culture solution for beauty is not obvious or the compatibility is poor The problem

Method used

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  • Human fibroblast regulating culture liquid flexible liposome, preparation method and application thereof
  • Human fibroblast regulating culture liquid flexible liposome, preparation method and application thereof
  • Human fibroblast regulating culture liquid flexible liposome, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] The preparation method of the flexible liposomes of human fibroblast regulating culture fluid is as follows:

[0062] Step 1. Prepare the human fibroblast conditioned culture concentrate: In a sterile environment, filter the serum-free human fibroblast conditioned culture with 0.8μm and 0.45μm capsule filters to remove the cells in the culture supernatant Fragments. Then the filtrate is obtained by ultrafiltration and concentration using an ultrafiltration device, and its total protein content is 20mg / mL;

[0063] Step 2. Weigh 4000mg lecithin, 4000mg triolein and 200mg of coenzyme Q10, dissolve in 16.4mL of absolute ethanol, and stir for 20min at a speed of 800r / min at 50℃ in a water bath to make it complete Dissolve to obtain alcohol phase solution;

[0064] Step 3. Weigh 400 mg of dipotassium glycyrrhizinate and 800 mg of arginine and add them to 96.6 mL of citrate buffer with pH 6.5 to obtain a mixed solution;

[0065] Step 4. Add the mixed solution obtained in step 3 dro...

Embodiment 2

[0071] The preparation method of the flexible liposomes of human fibroblast regulating culture fluid is as follows:

[0072] Step 1. Prepare the human fibroblast conditioned culture concentrate: In a sterile environment, filter the serum-free human fibroblast conditioned culture with 0.8μm and 0.45μm capsule filters to remove the cells in the culture supernatant Fragments. Then the filtrate is obtained by ultrafiltration and concentration using an ultrafiltration device, and its total protein content is 15mg / mL;

[0073] Step 2. Weigh out 10.0g lecithin, 1.2g triolein, 450mg lycopene and 450mg carotenoids, and dissolve them in 66.6mL absolute ethanol. Under 40℃ water bath conditions, at 650r / min Stir at a speed of 15min to make it completely dissolve to obtain an alcohol phase solution;

[0074] Step 3. Weigh 1.8 g of dipotassium glycyrrhizinate, 2.0 g of methionine and 1.3 g of caprylic acid and add them to 76.8 mL of pH 6.5 citric acid buffer to obtain a mixed solution;

[0075] S...

Embodiment 3

[0081] The preparation method of the flexible liposomes of human fibroblast regulating culture fluid is as follows:

[0082] Step 1. Prepare the human fibroblast conditioned culture concentrate: in a sterile environment, filter the serum-free human fibroblast conditioned culture with 0.8μm and 0.45μm capsule filters to remove the cells in the culture supernatant Fragments. Then the filtrate is obtained by ultrafiltration and concentration using an ultrafiltration device, and its total protein content is 10mg / mL;

[0083] Step 2. Weigh 8000mg lecithin, 500mg triolein, 1000mg ascorbyl palmitate, 1000mg vitamin E acetate, 1000mg guaiac resin and 1000mg curcumin, and dissolve them in 47.5mL of absolute ethanol at 30℃ Under the water bath condition, stir for 10min at a speed of 500r / min to make it completely dissolve to obtain an alcohol phase solution;

[0084] Step 3. Weigh 8.0g dipotassium glycyrrhizinate, 2.0g methionine, 2.5g sodium deoxycholate, 1.0g PEG400 and 2.5g polysorbate-80...

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Abstract

The invention discloses a human fibroblast regulating culture liquid flexible liposome, which is composed of the following components according to a mass percentage: 20.0% to 50.0% of human fibroblastregulating culture concentrate, 2.0% TO 8.0% of lecithin, 0.5-2.0% of triolein, 0.1%-4.0% of an antioxidant, 0.4%-8.0% of a stabilizer, 0.2 % to 8.0% of dipotassium glycyrrhizinate, 6.0% to 27.0% ofpropylene glycol, and 15.0 to 49.0% of a citrate buffer solution, wherein the sum of the mass percentages of the above components is 100%. The invention also discloses a preparation method of the above liposome, wherein the obtained liposome has uniform particle size and small PDI distribution, and can be stored at room temperature for 18 months, the activity of the human fibroblast regulating culture liquid is kept, and the problem that the human fibroblast-regulated culture solution has a short shelf life and a severe storage condition is solved. At the same time, the invention discloses anapplication of the human fibroblast regulating culture liquid flexible liposome in the technical field of cosmetic skin care.

Description

Technical field [0001] The present invention belongs to the technical field of biomedical cosmetology and skin care, and specifically relates to a human fibroblast regulating culture fluid flexible liposome, and specifically also relates to a preparation method of the human fibroblast regulating culture fluid flexible liposome, and the present invention also relates to the Application of flexible liposomes in the field of beauty and skin care technology. Background technique [0002] With the improvement of people’s living standards, people are paying more and more attention to beauty and maintenance. There are many types of beauty and maintenance on the market, but most of them can only stay on the surface of the skin to act as a physical barrier, and cannot penetrate the skin barrier. Solve skin problems, and the existing beauty products do not have biological activity, cannot effectively promote cell metabolism and improve skin problems. [0003] Human fibroblast regulating cul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/99A61K8/63A61K8/55A61K8/37A61K8/365A61K8/34A61K8/14A61Q19/00
CPCA61K8/99A61K8/553A61K8/375A61K8/63A61K8/345A61K8/365A61K8/14A61Q19/00
Inventor 胡春梅王海江王静肖萍
Owner 西安艾尔菲生物科技有限公司
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