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A kind of detection method of Arboria phenolic substance in Arboria plant or extract

A detection method and extraction technology, applied in the field of analytical chemistry, can solve the problems of high sample requirements and large interference, and achieve the effect of simple processing steps, pollution reduction and simple steps

Active Publication Date: 2021-08-03
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the low content of dendrogenols in the plant or extract of the dendrobium, the interference is greater during the detection, and the requirements for sample pretreatment are high, and the content of the dendrogenols in the plant or extract of the dendrobium The detection method has not been reported at present, therefore, the method dedicated to the detection of the Arboria phenolic substances in Arboria sativa plants or extracts is particularly important

Method used

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  • A kind of detection method of Arboria phenolic substance in Arboria plant or extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Use a sample tube containing 0.5g of water-removing agent anhydrous magnesium sulfate to collect 0.1 g of the extract (extract) of Saffron, add 1mL of methanol solution and shake fully for 10min, then take 1mL of the extraction solution into the chromatographic bottle, seal it quickly, and pass through the gas phase Chromatography-mass spectrometry analysis, quantitative according to component peak area;

[0018] The gas chromatographic conditions described are: the chromatographic column is a DB-5MS column, the length is 60 m × the inner diameter is 0.25 mm × the film thickness is 0.25 μ m; the carrier gas is helium (He); the temperature of the injection port is 170 ° C; constant flow mode , the column flow rate is 0.5 mL / min, the split ratio is 6:1; the programmed temperature rise is 40°C, hold for 1 min, and then rise to 150°C at a rate of 2°C / min, and hold for 5 min;

[0019] The mass spectrometry conditions are: ionization mode is electron bombardment source (EI), ...

Embodiment 2

[0022] Use a sample tube containing 1 g of water-removing agent anhydrous magnesium sulfate to collect 0.5 g of the plant Moss, add 5 mL of methanol solution and shake fully for 20 min, then take 1.5 mL of the extraction solution into a chromatographic bottle, quickly seal it, and perform gas chromatography-mass spectrometry. Analyze with instrument, quantify according to component peak area;

[0023] The gas chromatography conditions described are: HP-5MS column, length 60 m×inner diameter 0.25 mm×film thickness 0.25 µm; carrier gas is helium (He); inlet temperature is 180°C; constant flow mode, column The flow rate is 0.8mL / min, the split ratio is 8:1; the programmed temperature rise is 60°C, hold for 3 minutes, and rise to 160°C at a rate of 3°C / min, and hold for 8 minutes;

[0024] The mass spectrometry conditions are: the ionization mode is electron bombardment source (EI), the ion source temperature is 190°C, the ionization energy is 70 eV, the quadrupole temperature is ...

Embodiment 3

[0027] Use a sample tube containing 2 g of water-removing agent anhydrous magnesium sulfate to collect 1 g of the extract (absolute oil) of Sargassum, add 10 mL of methanol solution and shake fully for 30 minutes, then take 2 mL of the extraction solution into a chromatographic bottle, seal it quickly, and pass through gas chromatography. - Mass spectrometry analysis, quantification according to component peak area;

[0028] The gas chromatographic conditions described are: the chromatographic column is HP-5MS, the length is 60 m × the inner diameter is 0.25 mm × the film thickness is 0.25 μm; the carrier gas is helium (He); the inlet temperature is 190 ° C; The flow rate is 1.2 mL / min, the split ratio is 12:1; the programmed temperature rise is 70°C, hold for 5 minutes, and then rise to 180°C at a rate of 4°C / min, and hold for 10 minutes;

[0029] The mass spectrometry conditions are: ionization mode is electron bombardment source (EI), ion source temperature is 200°C, ioniza...

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Abstract

The present invention provides a method for detecting the saponinols in sassafras plants or extracts. The method adopts gas chromatography-mass spectrometry to measure sassy phenols in sassafras plants or extracts. The sample tube containing water-removing agent anhydrous magnesium sulfate is used to collect the plants or extracts of S. japonica, add 1-10mL of methanol solution and shake fully for 10-30min, then take 1-2mL of the extraction solution into the chromatographic bottle, seal it quickly, and pass through the gas chromatography. ‑Mass spectrometry analysis, external standard method for quantification; this detection method is suitable for 3,4‑dimethylphenol, 3‑methoxy‑5‑methylphenol, 3‑methoxy 2,5,6-trimethylphenol, 3,5-dihydroxytoluene, 2,5-dihydroxytoluene, 5-methoxy-2,3-xylenol, 2,5-dimethyl Simultaneous detection of ‑1,4‑benzenediol and other dendrols has the advantages of rapidity, accuracy, and simple operation.

Description

technical field [0001] The invention belongs to the technical field of analytical chemistry, and in particular relates to a method specially used for the detection of arboria phenolic substances in arborica plants or extracts. Background technique [0002] Moss ( Evernia furfuraceae ) belongs to Usneaceae ( Usneaceae ) Treeflower ( Ramalina ) plants are mainly distributed in Yunnan, Guizhou and other places. Due to the complex terrain and changeable climate of the place of origin, there are many kinds of tree mosses, and there are clusters of tree flowers ( Ramalina fastigiata ), crack tree flower ( Ramina Minuscula ) and tree flowers ( Ramalina commista ), etc., the surface is mainly gray-green or dark green, and the types and contents of chemical components are different. In industry, the moss extract is obtained by extracting the active ingredients in the moss mainly through hydrocarbon series solvents such as petroleum ether or benzene, and further processing w...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/72G01N30/86
CPCG01N30/02G01N30/06G01N30/72G01N30/8631
Inventor 张凤梅刘哲刘志华刘馨芮彤向能军唐石云李振杰蒋薇朱瑞芝司晓喜申钦鹏何沛尤俊衡王昆淼刘春波杨光宇苏钟璧
Owner CHINA TOBACCO YUNNAN IND
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