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Preparation method of dark septate endomycorrhiza fungal inoculant combination

A technology of dark-colored endophytic and mycorrhizal fungi with compartments, applied in biochemical equipment and methods, methods based on microorganisms, fungi, etc., can solve the problem of bacteria that cannot be operated in a large-scale factory and have a high infection rate of bacteria agents The shelf life of the agent and the instability of the infection effect of the bacterial agent are solved, and the large-scale factory operation is realized, the infection effect is stable, and the quality is good.

Active Publication Date: 2019-12-17
BAODING UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved in the present invention is to provide a method for preparing a dark-colored septated endomycorrhizal fungal agent combination, so as to solve the problem that the current dark-colored septated endomycorrhizal production method does not form a system framework and cannot realize large-scale The problem of factory operation and unstable infection effect of the prepared bacterial agent; to further achieve the purpose of low-cost preparation of bacterial agent, no pollution, high raw material utilization rate, high bacterial agent infection rate, and prolong the shelf life of the bacterial agent. Factory operation with septal endomycorrhizae

Method used

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  • Preparation method of dark septate endomycorrhiza fungal inoculant combination
  • Preparation method of dark septate endomycorrhiza fungal inoculant combination
  • Preparation method of dark septate endomycorrhiza fungal inoculant combination

Examples

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Effect test

Embodiment 1

[0041] S1: Collect well-growing, healthy and disease-free wild annual fibrous root herbaceous plant Potentilla chaotian, cut off the root system of Potentia chaotian, wash it under running water, and cut the root section about 1 cm in length. Carry out microscopic examination to the root segment, select the fresh root segment containing dark-colored endomycorrhizal mycelium with septum for microscopic examination, cut off both ends, and cut the root segment into 0.3-0.5 cm root segments, and use 75% After surface disinfection with ethanol for 3 minutes, wash with sterile water for 3 times, then disinfect with 8% sodium hypochlorite solution for 8 minutes, and wash with sterile water for 3 times.

[0042] S2: After peeling, washing and cutting the fresh potatoes, weigh three portions of 200g fresh potatoes, add 1000mL of distilled water to each portion and boil for 30min, then filter with four layers of gauze to obtain three portions of filtrate.

[0043] Take two of the filtrate...

Embodiment 2

[0054] S1: Collect a well-growing, healthy and disease-free wild annual fibrous root herbaceous plant, Nippon, cut off the root system of Nippon, wash it under running water, and cut the root segment of about 1 cm in length. Carry out microscopic examination to the root segment, select the fresh root segment containing dark-colored endomycorrhizal mycelium with septum for microscopic examination, cut off both ends, and cut the root segment into 0.3-0.5 cm root segments, and use 75% After surface disinfection with ethanol for 3 minutes, wash with sterile water for 3 times, then disinfect with 8% sodium hypochlorite solution for 8 minutes, and wash with sterile water for 3 times.

[0055] S2: After peeling, washing and cutting the fresh potatoes, weigh three portions of 200g fresh potatoes, add 1000mL of distilled water to each portion and boil for 30min, then filter with four layers of gauze to obtain three portions of filtrate.

[0056] Take two of the filtrates, add 20g of ag...

Embodiment 3

[0067] S1: Collect a well-growing, healthy and disease-free wild annual fibrous root herbaceous plant, C. chinensis, cut off the root system of C. chinensis, wash it under running water, and cut the root segments about 1 cm in length. Carry out microscopic examination to the root segment, select the fresh root segment containing dark-colored endomycorrhizal mycelium with septum for microscopic examination, cut off both ends, and cut the root segment into 0.3-0.5 cm root segments, and use 75% After surface disinfection with ethanol for 3 minutes, wash with sterile water for 3 times, then disinfect with 8% sodium hypochlorite solution for 8 minutes, and wash with sterile water for 3 times.

[0068] S2: After peeling, washing and cutting the fresh potatoes, weigh three portions of 200g fresh potatoes, add 1000mL of distilled water to each portion and boil for 30min, then filter with four layers of gauze to obtain three portions of filtrate.

[0069] Take two of the filtrates, add...

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Abstract

The invention discloses a preparation method of a dark septate endomycorrhiza fungal inoculant combination. The preparation method specifically comprises the steps of plant root system selecting and processing, cultivating system preparation, isolation and culture of strains, purifying culture of the strains, preparation and fermental cultivation of seed solutions of the strains, biocontrol bacteria powder preparation, and preparation of dark septate endomycorrhiza fungal inoculant combination. The dark septate endomycorrhiza fungal inoculant combination prepared by the invention has the advantages that the inoculant combination is good in quality, high in infection rate, high in colonization frequency, long in expiration date, and stable in infection effect, the preparation method can bewidely applied to the fields of scientific research, ecological control, soil improvement and restoration and the like, large-scale factory operation is achieved, and the industry requirement is met.

Description

technical field [0001] The invention relates to the technical field of microbial inoculum, in particular to a dark-colored septated endomycorrhizal fungal inoculum. Background technique [0002] Dark septal endomycorrhizal is a symbiosis of plant roots and fungi that widely exists in nature. Dark Septate Endiphytes (DSE) are small soil fungi that can colonize in plant roots and form mycorrhizal structures with plant roots. DSE is mainly distributed in extreme environments, such as areas polluted by heavy metals, desert areas, alpine regions, and polar regions. It has the function of mycorrhizal ecology, which can not only enhance the stress resistance of plants to biotic and abiotic factors, but also help plants survive in arid environments. It can maintain water and nutrient transport in the medium, and can enhance the stress resistance of plants and promote plant growth by increasing the activity of certain enzymes in plants. It is of great significance in restoring deser...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 吴艳清白菁华刘文静赵茹茹张士薇其他发明人请求不公开姓名
Owner BAODING UNIV
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