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Method for extracting Perilla frutescens leaf anthocyanin by using a ternary eutectic solvent

A technique of perilla leaf anthocyanin and low eutectic solvent, which is applied in the direction of organic chemistry and the like, can solve problems such as attenuation and residual organic solvent, and achieve the effects of increasing extraction rate, low vapor pressure, and good extraction ability.

Active Publication Date: 2019-12-20
ZHEJIANG GONGSHANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are residues of organic solvents and attenuation problems when microwaves penetrate the interior of the material, etc.

Method used

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  • Method for extracting Perilla frutescens leaf anthocyanin by using a ternary eutectic solvent
  • Method for extracting Perilla frutescens leaf anthocyanin by using a ternary eutectic solvent
  • Method for extracting Perilla frutescens leaf anthocyanin by using a ternary eutectic solvent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-6

[0033] Using polyols as hydrogen bond donors, mix the hydrogen bond donors with the hydrogen bond acceptor choline chloride ChCl, the ratio of the two is shown in Table 1, at N 2 The heating and stirring reaction was carried out under the condition of atmosphere and oil bath, wherein the heating temperature was 90°C, and the stirring time was 1h. After the reaction, a clear binary deep eutectic solvent DES could be obtained;

[0034] Into the three-necked bottle, add 5g of perilla leaf residue, 20mL of deep eutectic solvent, and 10mL of distilled water in sequence, and place it in an ultrasonic-microwave-ultraviolet combined instrument. The extraction temperature is 60°C. In the case of ultrasonic power and ultraviolet light, the reaction time is 20 minutes;

[0035] After the reaction is finished, the perilla leaf residue and the deep eutectic solvent rich in anthocyanins are layered. First centrifuge at 9000r / min and 4°C for 10min, then filter through a 0.22um pore filter m...

Embodiment 7-19

[0040] Use ethylene glycol as the hydrogen bond donor, sugar or carboxylic acid as the auxiliary ligand, mix the hydrogen bond donor with the hydrogen bond acceptor choline chloride ChCl, and then add the auxiliary ligand to mix. The ratio of the three is shown in Table 2 show, in N 2 Heating and stirring reaction is carried out in the atmosphere and under the condition of oil bath, wherein the heating temperature is 90°C, and the stirring time is 1h. After the reaction is completed, a clear ternary deep eutectic solvent DES can be obtained;

[0041] Into the three-necked bottle, add 5g of perilla leaf residue, 20mL of deep eutectic solvent, and 10mL of distilled water in sequence, and place it in an ultrasonic-microwave-ultraviolet combined instrument. The extraction temperature is 60°C. In the case of ultrasonic power and ultraviolet light, the reaction time is 20 minutes;

[0042] After the reaction is finished, the perilla leaf residue and the deep eutectic solvent rich i...

Embodiment 20-24

[0047] With ethylene glycol as the hydrogen bond donor and lactic acid as the auxiliary ligand, the hydrogen bond donor and the hydrogen bond acceptor choline chloride ChCl are mixed in a molar ratio of 3:1, and then the auxiliary ligand is added to mix, and the auxiliary ligand is mixed with The molar ratio of choline chloride is 0.7:1, in N 2 Heating and stirring reaction is carried out in the atmosphere and under the condition of oil bath, wherein the heating temperature is 90°C, and the stirring time is 1h. After the reaction is completed, a clear ternary deep eutectic solvent DES can be obtained;

[0048] In the there-necked flask, add perilla leaf powder 5g, then add a certain volume of deep eutectic solvent ChCl-ethylene glycol-lactic acid (1:3:0.7) and distilled water, the volume ratio of deep eutectic solvent and distilled water is 2: 1. The ratio of solid to liquid (g / ml) is shown in Table 3. Place it in an ultrasonic-microwave-ultraviolet light combination instrumen...

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Abstract

The invention discloses a Perilla frutescens leaf anthocyanin extraction method, and particularly relates to preparation of an eutectic solvent, and a method for ultrasonic wave-microwave-ultravioletlight assisted extraction of anthocyanin, wherein the eutectic solvent has advantages of diversified structure, biodegradability, environmental protection, easy preparation, renewable property, low melting point and the like, is an environmentally-friendly solvent, and can effectively reduce the solvent loss and obviously increase the content of anthocyanin at a low dosage; and the disadvantages of the single wave can be overcome through the ultrasonic wave-microwave-ultraviolet light combination, such that the method is an emerging technology integrating the advantages of ultrasonic wave extraction and microwave extraction, the solvent can penetrate through plant matrixes easily, and the effective components can be dissolved in the solvent easily.

Description

technical field [0001] The invention relates to the technical field of plant extraction, in particular to a method for extracting perilla leaf anthocyanins by using a ternary deep eutectic solvent. Background technique [0002] With the development of science and technology and the improvement of living standards, the demand for a new type of green solvent and equipment for extracting natural macromolecular products is increasing day by day. [0003] Deep eutectic solvent (DES) belongs to the ionic solution, which has the advantages of diverse structures, biodegradability, environmental protection, easy preparation, regeneration, and low melting point. It is a green solvent. The freezing point of deep eutectic solvents is mostly between -38 and Between 150°C, compared with the melting points of hydrogen bond donors and hydrogen bond acceptors that make up the deep eutectic solvent, the freezing point of the deep eutectic solvent can usually drop by 20 to 120°C. Therefore, i...

Claims

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Application Information

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IPC IPC(8): C07D311/62
CPCC07D311/62
Inventor 韩晓祥叶青杜银霜丰艺璇郑丽娜汪思田徐艺洺王彦波
Owner ZHEJIANG GONGSHANG UNIVERSITY
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