Microbial symbiotic culture inoculant and preparation method and application thereof

Abstract

The invention relates to the field of microorganisms, and particularly provides a microbial symbiotic culture inoculant and a preparation method and application thereof. The microbial symbiotic culture inoculant provided by the invention specifically comprises pseudomonas, bacillus subtilis, candida and xanthomonas. The inoculant is good in environmental adaptability and high in toxic pollution resistance, can be well applied to various petroleum pollution environments, achieves stable balance among strains through symbiotic culture, and can be prepared through large-scale fermentation. The target strains are co-cultured, a stable symbiotic system is formed by utilizing the interaction among the strains, the preparation method is simple, and the prepared microbial symbiotic culture inoculant is balanced and stable among the strains and high in environmental adaptability. The inoculant can treat petroleum sewage or petroleum sludge at a time, the treated petroleum sewage or petroleum sludge reaches the direct discharge standard, and the treatment cost is greatly reduced.

Description

technical field [0001] The present invention relates to the field of microorganisms, in particular, to a microbial symbiotic culture agent and a preparation method and application thereof. Background technique [0002] The use of microorganisms to degrade petroleum oil pollution is the current research hotspot of oil pollution treatment technology, but at present, there are many problems in the existing microbial decomposition of petroleum oil pollution technology. 2. The existing compound microbial inoculants are compounded from a single strain, and the growth conditions of each strain are different. The compound preparation requires the help of a variety of industrial methods, the process is complex, and the environmental adaptability of the inoculum is poor. , the ability to resist toxic pollution is weak, it cannot adapt to the changeable petroleum environment, the decomposition efficiency is low, and the treatment effect is poor; 3. After the existing bacterial agent de...

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Problems solved by technology

[0002] The use of microorganisms to degrade petroleum oil pollution is currently a research hotspot in oil pollution treatment technology, but at present, there are many problems in the existing technology of microbial decomposition of petroleum oil pollution: 1. The efficiency of single bacterial strains to degrade petroleum sludge is poor, and the combined application of multiple bacteria makes the treatment process complicated 1. Uncontrollable, high engineering cost; 2. The existing compound microbial bacterial agent is compounded from a single strain, and the growth conditions of each strain are different. The preparation of the compound requires a variety of industrial methods, the process is complicated, and the environmental adaptability of the bacterial agent is poor , the ability to resist toxic pollution is weak, unable to adapt to the changing petroleum environment, the decomposition efficiency is low, and the treatment effect is poor. high cost

[0003] In addition, because the petroleum components are very complex, and microbial decomposition has a certain bias, a certain type of bacteria cannot achieve rapid and thorough decomposition of multiple components of petroleum. A better way to treat petroleum oil pollution is the joint action of multiple microorganisms. However, in the prior art, the effect of co-application of different types of strains is often not achieved due to the different oxygen requirements of the strains, so that the microbial agents used in the prior art cannot achieve better treatment of oil and oil pollution due to the limitation of the strains. deal with

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