Enterococcus faecalis capable of reducing trimethylamine concentration and preparation method and application thereof
A technology of Enterococcus faecalis and trimethylamine, applied in the field of microorganisms, can solve problems such as lack of trimethylamine, and achieve the effect of reducing the concentration
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[0055] A method for preparing Enterococcus faecalis capable of reducing the concentration of trimethylamine in one embodiment, the method includes the following steps S110-S120.
[0056] S110. Preliminarily screen the fresh feces of healthy people to find the genus that can reduce the concentration of trimethylamine.
[0057] Specifically, a number of fresh feces dilutions from healthy people were inoculated into multiple trimethylamine-based brain-heart infusion broth (BHI broth) culture media and multiple brain-heart infusion broth (BHI broth) without trimethylamine added. In the base, anaerobic culture, multiple cultures were obtained, and then the DNA of multiple cultures were extracted, 16S rDNAV4 universal primers were selected for amplification, and the amplified products were sequenced. Then, the sequencing data were analyzed using the BIPES analysis process to obtain the genus that can reduce the concentration of trimethylamine.
[0058] It should be noted that the f...
Embodiment 1
[0099] (1) Randomly select the fresh feces of 11 healthy people, and take 1g each to prepare 10 5 Diluted liquid, and recorded as feces sample 1 ~ feces sample 11, respectively inoculated the diluted liquid into the brain heart extract broth (BHI broth) added with 4g / L trimethylamine according to the volume ratio of feces diluted liquid and liquid medium 1:25 ) medium and BHI broth medium without adding trimethylamine.
[0100] (2) A plurality of above-mentioned two culture mediums after inoculation are placed in anaerobic box (10% H 2 , 5% CO 2 , 85%N 2 ) at 37°C for 7 days in anaerobic culture, and multiple culture products were obtained.
[0101] (3) Use the Bacterial Genomic DNA Magnetic Beads Extraction Kit of Shenzhen Yirui Biotechnology Co., Ltd. to extract the DNA of multiple culture products.
[0102](4) 16S rDNA V4 universal primers were selected to amplify the 16S rDNA V4 fragments of multiple culture products respectively to obtain multiple amplification produc...
Embodiment 2
[0111] (1) Randomly select 1 g of the feces of two healthy people to make 10 5 Diluted solution, then take 100 microliters to inoculate on the BHI agar medium of XX mL respectively, and place in anaerobic box (10% H 2 , 5% CO 2 , 85%N 2 ) at 37°C for 7 days to obtain the culture to be screened.
[0112] (2) Purify the culture to be screened by streaking on the plate until only a single colony of one form grows on the BHI agar medium.
[0113] (3) Pick multiple single colonies to extract their DNA, respectively amplify and measure the 16S rDNA sequences of multiple single colonies, compare the results with the 16S rDNA sequences of Enterococcus faecalis, and determine Results The 16S rDNA sequence identical to that of Enterococcus faecalis was Enterococcus faecalis, and a strain of Enterococcus faecalis was screened. The Enterococcus faecalis was preserved in Guangdong Microbial Culture Collection Center (GDMCC) on April 23, 2018, address: 5th Floor, Building 59, Compound, ...
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