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A method for improving photosynthetic efficiency of plants

A technology for photosynthetic efficiency and plants, applied in botany equipment and methods, biochemical equipment and methods, plant peptides, etc., can solve the problems of reduced drought tolerance, reduce photorespiration, improve photosynthetic efficiency, and increase plant biomass or yield effect

Active Publication Date: 2022-06-28
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] However, our study found that plants overexpressing GDH and MS genes in chloroplasts, while inhibiting the expression of PLGG1 gene, had significantly reduced drought tolerance compared with the control

Method used

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  • A method for improving photosynthetic efficiency of plants
  • A method for improving photosynthetic efficiency of plants
  • A method for improving photosynthetic efficiency of plants

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1, the construction of carrier

[0032] The GDH genes of the present invention can be derived from prokaryotes or eukaryotes. The GDH genes provided by the present invention include but are not limited to the genes shown in Table 2. In order to construct the transformation vector, the E. coli-derived GDH gene and the corresponding terminator sequence were artificially synthesized, including the chloroplast signal peptide, the GDH coding gene and the terminator. The nucleotide sequence is shown in SEQID NO. ' ends are provided with BamHI and KpnI sites, respectively. The artificially synthesized GCL gene includes a chloroplast signal peptide, a GCL coding gene and a terminator, the nucleotide sequence is shown in SEQ ID NO. 8, and the 5' end and the 3' end are respectively provided with BamHI and HindIII sites. The artificially synthesized TSR gene includes a chloroplast signal peptide, a TSR coding gene and a terminator, the nucleotide sequence is shown in S...

Embodiment 2

[0039] Example 2, rice transformation

[0040] The method of obtaining transgenic rice is to use the existing technology (Lu Xiongbin, Gong Zuxun (1998) Life Science 10: 125-131; Liu Fan et al. (2003) Molecular Plant Breeding 1: 108-115). The mature and plump "Xiushui-134" seeds were selected and shelled to induce callus as the transformation material. Take the Agrobacterium plates constructed in Example 1 containing the plasmids pCambia1300-bar-GDH-GCL-TSR-OsBASS-RNAi (GGTOsBi) and pCambia1300-bar-GDH-GCL-TSR-OsPGGL1-RNAi (GGTOsPi) respectively. Pick a single colony to inoculate and prepare for transformation with Agrobacterium. The callus to be transformed is put into the Agrobacterium bacterium liquid with an OD of about 0.6 (the preparation of the Agrobacterium bacterium liquid: inoculate the Agrobacterium into the culture medium, and cultivate to an OD of about 0.6; the medium consists of 3 g / L K 2 HPO 4 , 1g / L NaH 2 PO 4 , 1g / L NH 4 Cl, 0.3g / L MgSO 4 ·7H 2 O, 0.1...

Embodiment 3

[0041] Example 3. Soybean Transformation

[0042] The steps used here to obtain transgenic soybeans were derived from existing techniques (Deng et al., 1998, PlantPhysiology Communications 34:381-387; Ma et al., 2008, Scientia Agricultura Sinica 41:661-668; Zhou et al., 2001 , Journal of Northeast Agricultural University 32:313-319). Select healthy, plump and mature soybeans of "Tianlong No. 1", sterilize them with 80% ethanol for 2 minutes, rinse with sterile water, and place them in a desiccator filled with chlorine gas (produced by the reaction of 50ml NaClO and 2ml concentrated HCl) Sterilize for 4-6 hours. The sterilized soybeans were sown into B5 medium in an ultra-clean workbench, and cultivated at 25°C for 5 days, while the optical density was 90-150μmol photons / m 2 ·s level. When the cotyledons turn green and burst the seed coat, sterile sprouts will grow. The sprouts with the hypocotyls removed were cut in half in length so that both explants had cotyledons and e...

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Abstract

The invention discloses a method for improving photosynthetic efficiency of plants. The method comprises: inhibiting or knocking out the sodium bile acid co-transporter gene in plants, and simultaneously overexpressing glycolate dehydrogenase gene, glyoxylate carboxylase gene and Tartrate semialdehyde reductase gene. The present invention creatively finds that inhibiting the expression of BASS6 gene in plants, combined with the overexpression of glycolate dehydrogenase gene, glyoxylate carboxylase gene and tartrate semialdehyde reductase gene in chloroplast, can significantly reduce photorespiration and improve plant photosynthetic efficiency, increase plant biomass or yield, and more importantly, the drought tolerance of this transgenic plant is significantly higher than that of the plant that inhibits the expression of PLGG1 gene, and the biomass or yield under drought conditions is increased by 3% compared with the non-transgenic control.-45 %.

Description

(1) Technical field [0001] The invention relates to a method for improving the photosynthetic efficiency of plants. The photosynthetic efficiency, biomass or yield of plants can be improved by means of transgenic methods, and plant planting resources can be improved. (2) Background technology [0002] The increase in the number of human beings and the improvement of living standards require more food and feed to be consumed, which requires more food to be harvested on limited land. Therefore, it is very important to breed new high-yielding plant varieties. [0003] The photosynthesis products of the whole plant are all derived from the enzyme catalyzed CO 2 converted to organic carbon compounds. Ribulose 1,5-diphosphate carboxylase / oxygenase (RubisCO) is a carboxylase in the Calvin-Benson (CB) cycle. As RubisCO and CO 2 or O 2 can react, RubisCO and O 2 The reaction produces phosphoglycolic acid, which enters the photorespiration cycle, which results in a waste of carb...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/82C12N15/84C12N15/29C12N15/53C12N15/60A01H5/00A01H5/10A01H6/46A01H6/54
CPCC12N15/8218C12N15/8273C12N15/8269C12N9/0006C12N9/88C07K14/415C12Y101/99014C12Y401/01047C12Y101/0106
Inventor 张先文王东芳向雅琴沈志成
Owner ZHEJIANG UNIV