Small molecule peptide for reducing blood pressure and preparation method and application thereof
A small molecular peptide and blood pressure lowering technology, applied in the field of bioengineering, can solve the problems of low conversion rate and low enzymatic hydrolysis efficiency, achieve the effect of reducing energy consumption and pollution discharge, and reducing material cost
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Embodiment 1
[0046] A kind of antihypertensive small molecule peptide, its preparation method comprises the following steps:
[0047] (1) Eggs are shelled, take 1000mL of egg solution after shelling, add n-hexane-ethanol (volume ratio 1:1) mixture in a ratio of 1:2 by volume, stir continuously at 4°C, and extract egg oil and lecithin , after 2h, let it stand for 30min, then carry out solid-liquid separation, dry and pulverize the solid at low temperature, analyze the protein content in the extracted raw material by Kjeldahl method, and recover n-hexane-ethanol;
[0048] (2) Weigh 100g of extracted egg protein powder, disperse it in 1L of deionized water, then add 100mM NaOH, react at 37°C for 1h for dephosphorization treatment, and quickly adjust the egg yolk solution to protease with hydrochloric acid The appropriate range of hydrolysis, and heating with a constant temperature water bath to maintain the enzyme action temperature, according to the mass ratio of egg protein powder and enzym...
Embodiment 2
[0056] A kind of antihypertensive small molecule peptide, its preparation method comprises the following steps:
[0057] (1) Eggs are shelled, take 1000mL of egg solution after shelling, add n-hexane-ethanol (volume ratio 1:1) mixture in a ratio of 1:2 by volume, stir continuously at 4°C, and extract egg oil and lecithin , after 2h, let it stand for 30min, then carry out solid-liquid separation, dry and pulverize the solid at low temperature, analyze the protein content in the extracted raw material by Kjeldahl method, and recover n-hexane-ethanol;
[0058] (2) Weigh 100g of extracted egg protein powder, disperse it in 1L of deionized water, then add 100mM NaOH, react at 37°C for 1h for dephosphorization treatment, and quickly adjust the egg yolk solution to protease with hydrochloric acid The appropriate range of hydrolysis, and heating with a constant temperature water bath to maintain the enzyme action temperature, according to the mass ratio of egg protein powder and enzym...
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