Organosilicon/sarcodon quel polysaccharide nano-composite, preparation method and application thereof

A nano-composite, black tiger palm bacteria technology, applied in the direction of organic active ingredients, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc. The application of edible fungus polysaccharide and other problems can achieve the effect of improving cell morphology, simple preparation method, and no toxic and side effects.

Active Publication Date: 2020-01-03
ANHUI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the biological activity of natural edible fungus polysaccharides is limited, and the ideal scavenging effect is often not achieved, which limits the application of edible fungus polysaccharides.

Method used

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  • Organosilicon/sarcodon quel polysaccharide nano-composite, preparation method and application thereof
  • Organosilicon/sarcodon quel polysaccharide nano-composite, preparation method and application thereof
  • Organosilicon/sarcodon quel polysaccharide nano-composite, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] This embodiment provides a preparation method of organosilicon / polysaccharide nanocomposite of Scutellaria nigricans, comprising the following steps:

[0052] (1) Preparation of crude polysaccharide

[0053] (11) Pulverize the sporocarp of the black tiger palm, cross a 60-mesh sieve to obtain the black tiger palm powder; Add the distilled water to obtain the extraction stock solution, then ultrasonically treat the extraction stock solution for 20 minutes, then extract at 80°C for 2 hours, finally use four layers of gauze to filter the extraction stock solution to obtain the supernatant, and repeat the extraction for 3 times, the supernatant was combined to obtain the extract;

[0054] (12) Using rotary steaming, the extract is concentrated to obtain a concentrated extract, and then absolute ethanol is added to the concentrated extract, the volume of the concentrated extract and absolute ethanol The ratio is 1:4, after stirring and mixing, let it stand at 4°C for 12 ho...

Embodiment 2

[0069] This embodiment tests the free radical scavenging ability of the silicone / polysaccharide nanocomposite prepared in Example 1

[0070] (1) Scavenge DPPH free radicals

[0071] Prepare the organosilicon / Pipella nigra polysaccharide nanocomposite solution with the concentration of 0.4mg / ml, 0.8mg / ml, 1.2mg / ml, 1.6mg / ml and 2.0mg / ml respectively, and prepare the solution with the concentration of 0.22mmol / l For the methanol solution of DPPH, take 2ml each of the organosilicon / Salpinella polysaccharide nanocomposite solutions with different concentrations, and mix them with 2ml of the methanol solution of DPPH respectively. Shake and mix well and incubate at room temperature for 30min, measure absorbance at 517nm, ascorbic acid is used as a positive control, set up three parallel experiments for each group, and take the average value of absorbance.

[0072] DPPH free radical scavenging ability = (1-(A 1 -A 2 ) / A 0 )×100%

[0073] in,

[0074] A 0 - Absorbance value of...

Embodiment 3

[0094] This example tests the protective effect of the silicone / polysaccharide nanocomposite prepared in Example 1 on Helf cells from oxidative damage

[0095] (1) Toxicity detection of organosilicon / polysaccharide nanocomposite of Scutellaria nigrum to normal cells

[0096] Helf cells in good growth state were inserted into the 96-well plate, and 3×10 cells were added to each well. 3 cells. at 5% CO 2 After adapting to the culture in the incubator for 24 hours, set up 5 groups of organosilicon / polysaccharide nanocomposite groups, 1 group of positive control group and 1 group of blank control group, and 5 groups of organosilicon / polysaccharide nanocomposites. In the compound administration group, the concentrations of the organosilicon / polysaccharide nanocomposite were 0.2mg / ml, 0.4mg / ml, 0.6mg / ml, 0.8mg / ml, 1.0mg / ml respectively, and the positive control group The concentration of ascorbic acid in the medium is 0.2mg / ml, after processing for 24h, remove culture fluid, util...

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Abstract

The invention discloses a preparation method of an organosilicon/sarcodon quel polysaccharide nano-composite. The preparation method comprises the following steps that preparation of sarcodon quel crude polysaccharide is conducted; preparation of sarcodon quel pure polysaccharide is conducted, specifically, the sarcodon quel crude polysaccharide is placed in a chromatographic column to be separated to obtain a polysaccharide component, and the polysaccharide component is freezed and dried for 72h to obtain the sarcodon quel pure polysaccharide; and organosilicone and the sarcodon quel pure polysaccharide are respectively dissolved in water, and then are respectively dissolved to obtain an organosilicon solution and a sarcodon quel pure polysaccharide solution, then the organosilicon solution is mixed with the sarcodon quel pure polysaccharide solution to obtain an organosilicon/sarcodon quel polysaccharide mixed solution, and the organosilicon/sarcodon quel polysaccharide mixed solution is subjected to an ultrasonic treatment to obtain the organosilicon/sarcodon quel polysaccharide nano-composite. According to the preparation method, the better free radical scavenging effect of theorganosilicon/sarcodon quel polysaccharide nano-composite is achieved, cell morphology is improved, resistance to hydrogen peroxide damage is improved, the toxic side effect is avoided, and the preparation method is simple.

Description

technical field [0001] The invention belongs to the technical field of food processing, and in particular relates to a nano-composite of organosilicon / polysaccharide from Scutellaria nigra, a preparation method and an application. Background technique [0002] If cells are constantly exposed to oxygen, they will continuously produce "oxygen free radicals". When cells metabolize in an environment that requires oxygen, they will also produce an antioxidant free radical defense system to resist the damage caused by oxygen free radicals to cells. No matter how sound the defense system against oxygen free radicals in cells is, as long as nucleic acid (DNA) and proteins damaged by oxygen free radicals accumulate to a considerable amount during the survival of cells, they will lead to various age-dependent diseases , such as arteriosclerosis, arthritis, neurodegenerative diseases, and cancer. [0003] Oxygen free radicals induce cell mutations, resulting in the initiation and pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L31/00A23L33/00A61K31/715A61K47/24A61P39/06
CPCA23L31/00A23L33/00A61K31/715A61K47/24A61P39/06A23V2002/00A23V2250/208A23V2200/30
Inventor 陈蕾董会彬陈彦蒋云杨军岭张文娜王亚
Owner ANHUI UNIVERSITY
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