Collagen extraction and purification system

A collagen and raw material technology, applied in animal/human protein, biochemical instruments, organic chemistry, etc., can solve the problems of poor water solubility, low filtration level, high salt content, etc., and achieve the effect of good molecular weight uniformity and improved purity

Inactive Publication Date: 2020-01-03
祁松
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, there are many defects in the traditional collagen preparation process, such as: (1) the volume of the extract is large, and the production cycle of the product is long; (2) the enzymatic hydrolysis solution is not completely removed, which affec...

Method used

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  • Collagen extraction and purification system

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Embodiment

[0015] See figure 1 , the invention discloses a collagen extraction and purification system, comprising the following devices interconnected in sequence: a degreasing tank and a rinsing device for degreasing raw materials, a crusher for crushing degreased raw materials, and enzyme processing for crushed raw materials Enzyme hydrolysis tank for hydrolysis treatment, refrigerated centrifuge for refrigerated centrifugation treatment of enzymatic hydrolysis solution, alkali washing tank for alkaline washing treatment of precipitate obtained by refrigerated centrifugation, salting-out unit for salting-out treatment of alkaline washing solution, Filtration unit and dialysis unit for purification of crude collagen after salting out, freeze-drying unit for solidification of purified collagen.

[0016] Among them, the raw materials are fish skin, pig skin and other collagen-rich, low-cost and easy-to-obtain materials, which are added to the degreasing tank, added with chloroform / ethano...

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Abstract

The invention discloses a collagen extraction and purification system. The collagen extraction and purification system comprises the following devices of a degreasing tank, a leaching device, a crusher, an enzymolysis tank, a refrigerated centrifuge, an alkali-washed tank, a salting-out unit, a filter unit, a dialysis unit and a freeze drying unit which communicate with each other in sequence, wherein the degreasing tank and the leaching device degrease raw materials, the crusher crushes the degreased raw materials, the enzymolysis tank conducts enzymolysis treatment on the crushed raw materials, the refrigerated centrifuge conducts refrigerated centrifugation treatment on enzymatic hydrolysate, the alkali-washed tank conducts alkali washing treatment on precipitates obtained by refrigerated centrifugation, the salting-out unit conducts salting-out treatment on alkali wash water, the filter unit and the dialysis unit purify coarse collagen subjected to salting-out, and the freeze drying unit cures a purified collagen solution. The collagen extraction and purification system is high in extraction efficiency, and the purity of obtained products is high.

Description

technical field [0001] The invention relates to the field of collagen extraction and processing, in particular to a system for extracting and purifying recombinant human collagen. Background technique [0002] As we all know, the existing collagen process is as follows: in the collagen extraction process, the raw materials are first mixed with an appropriate amount of water, steamed at high temperature, and an appropriate amount of enzymatic hydrolysis agent is added under appropriate pH conditions for enzymatic extraction. Afterwards, carry out fine filtration; the filtrate enters the evaporation concentration system for concentration and dehydration, and after the concentrated liquid reaches a certain concentration, it enters the spray drying equipment, and the obtained powder is collagen. [0003] However, there are many defects in the traditional collagen preparation process, such as: (1) the volume of the extract is large, and the production cycle of the product is long...

Claims

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Application Information

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IPC IPC(8): C07K14/78C07K1/34C07K1/02C07K1/00C12M1/40C12M1/12C12M1/02
CPCC07K14/78C12M21/18C12M27/02C12M33/14C12M41/14
Inventor 祁松
Owner 祁松
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