Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Placenta preservation solution and preparation method thereof

A technology for preserving liquid and placenta, applied in the biological field, can solve the problems of short preservation time, stem cell yield and activity effects, and achieve the effects of low cost, simple proportioning, and improved efficiency

Inactive Publication Date: 2020-01-10
QINGDAO RESTORE BIOTECHNOLOGY CO LTD
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the problems of short storage time and affected stem cell yield and activity in the prior art, and provide a placenta preservation solution, which has simple proportioning, low cost and convenient use

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Placenta preservation solution and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] High-sugar DMEM dry powder medium 13.4g, sodium bicarbonate 2.438g, insulin 10mg, penicillin 200mg, streptomycin 200mg, amphotericin 5mg, heparin sodium injection 1.5g, rapamycin 50mg, trametinib 0.6mg , Paeoniflorin 5mg, and the balance is water for injection.

[0019] The preparation method of this placenta preservation solution is as follows:

[0020] (a) Pour 13.4 g of high-sugar DMEM dry powder medium (brand: GIBCO, product number 12100-046) into a container, wash off the residual medium in the bag with a small amount of water for injection, and put it into the container. Add water for injection to 950ml, stir gently to dissolve;

[0021] (b) Add 2.438 g sodium bicarbonate;

[0022] (c) Gently stir to dissolve, add water for injection to 1L;

[0023] (d) adjust the pH value to neutrality with 1mol / L sodium hydroxide solution or 1mol / L hydrochloric acid solution;

[0024] (e) Insulin 10mg (brand Sigma, article number I0908), penicillin 200mg (brand Amresco, arti...

Embodiment 2

[0027] High-sugar DMEM dry powder medium 13.4g, sodium bicarbonate 2.438g, insulin 2mg, penicillin 100mg, streptomycin 100mg, amphotericin 2.5mg, heparin sodium injection 0.5g, rapamycin 10mg, trametinib 0.3 mg, paeoniflorin 2mg, and the balance is water for injection.

[0028] The preparation method of this placenta preservation solution is as follows:

[0029] (a) Pour 13.4 g of high-sugar DMEM dry powder medium into a container, wash off the residual medium in the bag with a small amount of water for injection, and put it into the container. Add water for injection to 950ml, stir gently to dissolve;

[0030] (b) Add 2.438 g sodium bicarbonate;

[0031] (c) Gently stir to dissolve, add water for injection to 1L;

[0032] (d) adjust the pH value to neutrality with 1mol / L sodium hydroxide solution or 1mol / L hydrochloric acid solution;

[0033] (e) Add 2 mg of insulin, 100 mg of penicillin, 100 mg of streptomycin, 2.5 mg of amphotericin, 0.5 g of heparin sodium injection, 1...

Embodiment 3

[0036] High-sugar DMEM dry powder medium 13.4g, sodium bicarbonate 2.438g, insulin 6mg, penicillin 150mg, streptomycin 150mg, amphotericin 3.5mg, heparin sodium injection 1g, rapamycin 30mg, trametinib 0.45mg , Paeoniflorin 3.5mg, and the balance is water for injection.

[0037] The preparation method of this placenta preservation solution is as follows:

[0038] (a) Pour 13.4 g of high-sugar DMEM dry powder medium into a container, wash off the residual medium in the bag with a small amount of water for injection, and put it into the container. Add water for injection to 950ml, stir gently to dissolve;

[0039] (b) Add 2.438 g sodium bicarbonate;

[0040] (c) Gently stir to dissolve, add water for injection to 1L;

[0041] (d) adjust the pH value to neutrality with 1mol / L sodium hydroxide solution or 1mol / L hydrochloric acid solution;

[0042] (e) Add 6 mg of insulin, 150 mg of penicillin, 150 mg of streptomycin, 3.5 mg of amphotericin, 1 g of heparin sodium injection, 30...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a placenta preservation solution. The placenta preservation solution is used for preserving placenta tissue samples after collection and before separation, and mainly comprisesa high-sugar DMEM dry powder culture medium, sodium bicarbonate, insulin, penicillin, streptomycin, amphotericin, a heparin sodium injection, rapamycin, trametinib and paeoniflorin. The placenta preservation solution has the advantages of simplicity in proportioning, low cost and convenience in use, keeping of the activity of stem cells in the placenta tissue sample, realization of effective preservation of the stem cells at 0-8 DEG C for at least 120 h, small influences of time on the activity of the separated stem cells, great reduction of the time limit from collection to preparation of the tissue samples, and effective reduction of the pollution probability.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a preservation solution for preserving placental tissue samples. Background technique [0002] Placental stem cells have broad prospects for clinical application, and have attracted the attention of numerous international experts from the very beginning. Placental stem cells refer to stem cells present in placental tissue, including hematopoietic stem cells, mesenchymal stem cells and other stem cells. Placental hematopoietic stem cell transplantation can treat malignant blood diseases, and the number of hematopoietic stem cells in the placenta is abundant, which is 5 to 10 times that of umbilical cord blood. hematopoietic system. Compared with stem cells from other sources, placental mesenchymal stem cells also have the following advantages: 1) low immunogenicity and immunosuppressive effect; 2) strong immunoregulatory effect, which can regulate the immune status of patients; The...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 陈梦梦张炳强邹伟刘玉欣辛毅付学奇
Owner QINGDAO RESTORE BIOTECHNOLOGY CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com