Placenta preservation solution and preparation method thereof

Abstract

The invention discloses a placenta preservation solution. The placenta preservation solution is used for preserving placenta tissue samples after collection and before separation, and mainly comprisesa high-sugar DMEM dry powder culture medium, sodium bicarbonate, insulin, penicillin, streptomycin, amphotericin, a heparin sodium injection, rapamycin, trametinib and paeoniflorin. The placenta preservation solution has the advantages of simplicity in proportioning, low cost and convenience in use, keeping of the activity of stem cells in the placenta tissue sample, realization of effective preservation of the stem cells at 0-8 DEG C for at least 120 h, small influences of time on the activity of the separated stem cells, great reduction of the time limit from collection to preparation of the tissue samples, and effective reduction of the pollution probability.

Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a preservation solution for preserving placental tissue samples. Background technique

[0002] Placental stem cells have broad prospects for clinical application, and have attracted the attention of numerous international experts from the very beginning. Placental stem cells refer to stem cells present in placental tissue, including hematopoietic stem cells, mesenchymal stem cells and other stem cells. Placental hematopoietic stem cell transplantation can treat malignant blood diseases, and the number of hematopoietic stem cells in the placenta is abundant, which is 5 to 10 times that of umbilical cord blood. hematopoietic system. Compared with stem cells from other sources, placental mesenchymal stem cells also have the following advantages: 1) low immunogenicity and immunosuppressive effect; 2) strong immunoregulatory effect, which can regulate the immune status of patients; The...

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