Purification and separation method of N-acetyl-D-glucosamine

Abstract

The invention belongs to the field of pharmaceutical chemicals, and discloses a purification and separation method of N-acetyl-D-glucosamine. The purification and separation method of the N-acetyl-D-glucosamine comprises the following steps: adding lactic acid glycine hydrochloride and hydrochloric acid into chitin powder, performing mixing, and performing filtering to obtain a filtrate; performing heating, adding hydrochloric acid dropwise, performing stirring, performing cooling, and performing decoloring to obtain D-glucosamine hydrochloride; adding an ethanol solution of sodium ethoxide, and performing filtering to obtain a free D-glucosamine solution; adding acetic anhydride to obtain a solution containing the N-acetyl-D-glucosamine; and performing concentration, performing cooling, performing crystallizing, performing filtration to obtain an N-acetyl-D-glucosamine crude product, performing soaking by using absolute ethyl alcohol, performing stirring, performing filtration, and performing drying to obtain the high-purity N-acetyl-D-glucosamine. According to the method, the N-acetyl-D-glucosamine with the purity of 99.95% or above can be obtained, the technological process is simple, the post-treatment is easy to realize, the cost is low, the used alcohol solvent can be recycled, and the method is environmentally friendly, economical and suitable for large-scale promotion and application.

Description

technical field [0001] The invention relates to the field of medicine and chemical industry, in particular to a purification and separation method of N-acetyl-D-glucosamine. Background technique [0002] Amino sugars are generally used as monosaccharide residues in complex oligosaccharides and polysaccharides, glucosamine is an amino derivative of monosaccharide glucose, and N-acetyl-D-glucosamine is an acetylated derivative of glucosamine. It is a special monosaccharide with high sweetness, has reducing properties, and is also an important precursor for the synthesis of bifidus factors. It has many important physiological functions in organisms and is clinically used as a drug for treating rheumatism and rheumatoid arthritis. , It can also be used as food antioxidant and infant food additive or sweetener for diabetic patients. At the same time, it can enhance the function of the human immune system, inhibit the excessive growth of cancer cells or fibroblasts, and inhibit a...

AI Technical Summary

Problems solved by technology

The preparation of N-acetyl-D-glucosamine by microbial fermentation requires the cultivation of microbial strains, followed by fermentation, separation and other processes. The whole process is complicated, the operation is cumbersome, and the yield is very low, which is not conducive to industrial production. Chemical production of N -Acetyl-D-glucosamine, the preparation process requires a lot of toxic and harmful chemical reagents, not only there are chemical residues in the product N-acetyl-D-glucosamine, but also chemical pollution is not conducive to environmental protection, and the whole process is extremely prone to combustion, explosion, etc. Accident, enzymatic hydrolysis to prepare N-acetyl-D-glucosamine, no matter what kind of enzyme is needed, it needs to increase the industrial cost, and the yield is also very low, which is not conducive to industrial production. Three kinds of N-acetyl-D-glucosamine preparation method, there is the problem of low product purity