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Steady-state petunidin-3-O-glucoside derivative and preparation method thereof

A technology of petunidin and glycoside derivatives, which is applied in the preparation of sugar derivatives, sugar derivatives, sugar derivatives, etc., can solve the problems of poor stability of anthocyanins, and achieve good light and heat stability and cell inhibition rate increase, the effect of increasing the cell proliferation rate

Inactive Publication Date: 2020-01-10
CHANGCHUN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In view of this, in order to solve the technical problem of poor anthocyanin stability in the prior art, the present invention provides a stabilized petunidin-3-O-glucoside derivative and its preparation method

Method used

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  • Steady-state petunidin-3-O-glucoside derivative and preparation method thereof
  • Steady-state petunidin-3-O-glucoside derivative and preparation method thereof
  • Steady-state petunidin-3-O-glucoside derivative and preparation method thereof

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preparation example Construction

[0035] The preparation method of the stabilized petunidin-3-O-glucoside derivative of the present invention comprises the following steps:

[0036] Step 1, destemming the mountain grapes, beating, and obtaining the mountain grape slurry;

[0037] Step 2, using a non-thermal extraction technique to extract the anthocyanins in the grape sap to obtain an anthocyanin solution;

[0038] Step 3, centrifuging the anthocyanin solution, taking the supernatant, concentrating under reduced pressure, eluting with a macroporous resin, combining the eluents, concentrating under reduced pressure, and freeze-drying to obtain a purified anthocyanin;

[0039] Step 4. Dissolve the purified anthocyanin in methanol solution containing 0.5-1% (v / v) HCl, separate by preparative liquid chromatography, collect the elution peaks, and freeze-dry to obtain Petunia-3-O- Glucoside;

[0040] Step 5. Dissolve caffeic acid and petunienin-3-O-glucoside in an organic solvent in a mass ratio of 1:(3-5), mix we...

Embodiment 1

[0050] Step 1, destemming the mountain grapes, beating, and obtaining the mountain grape slurry;

[0051] Step 2, using a high-voltage pulsed electric field to extract the anthocyanins in the vine grape slurry to obtain an anthocyanin solution; wherein, the extraction solvent is 0.1% hydrochloric acid-75% ethanol solution, the liquid-to-material ratio is 9:1w / w, and the electric field strength is 15Kv / cm , pulse number 4;

[0052] Step 3, centrifuge the anthocyanin solution, take the supernatant, concentrate under reduced pressure (to a relative density of 1.1, 50°C), elute with a macroporous resin, combine the eluents, and concentrate under reduced pressure (to a relative density of 1.3 , 50°C), freeze-dried to obtain purified anthocyanins; wherein, the conditions for preparative liquid chromatography separation were: chromatographic column: SunFire Prep C 18 Column, size 19mm×50mm, 5μm; detection wavelength 530nm; column temperature 25°C; flow rate 5mL / min; 15%B; 5~8min, 1...

Embodiment 2

[0056] Step 1, destemming the mountain grapes, beating, and obtaining the mountain grape slurry;

[0057] Step 2, using a high-voltage pulsed electric field to extract the anthocyanins in the vine grape slurry to obtain an anthocyanin solution; wherein, the extraction solvent is 0.1% hydrochloric acid-75% ethanol solution, the liquid-to-material ratio is 9:1w / w, and the electric field strength is 15Kv / cm , pulse number 4;

[0058] Step 3, centrifuge the anthocyanin solution, take the supernatant, concentrate under reduced pressure (to a relative density of 1.1, 50°C), elute with a macroporous resin, combine the eluents, and concentrate under reduced pressure (to a relative density of 1.2 , 50°C), freeze-dried to obtain purified anthocyanins;

[0059] Step 4. Dissolve the purified anthocyanin in methanol solution containing 0.5-1% (v / v) HCl, separate by preparative liquid chromatography, collect the elution peaks, and freeze-dry to obtain Petunia-3-O- Glucoside; Wherein, the ...

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Abstract

The present invention discloses a steady-state petunidin-3-O-glucoside derivative and a preparation method thereof, belongs to the technical field of compounds and preparation thereof, and solves a problem that existing anthocyanins are poor in stability. The derivative has a structural formula shown in a formula I. The preparation method is as follows: firstly stems of vitis amurensis are removedand the vitis amurensis is beaten into slurry to obtain vitis amurensis slurry liquid; then a non-thermal extraction technology is used to extract anthocyanins in the vitis amurensis slurry liquid toobtain an anthocyanin solution; then the anthocyanin solution is centrifuged, a supernatant is taken, concentration under reduced pressure, elution with a macroporous resin, eluate blending, concentration under reduced pressure, and freeze-drying are conducted to obtain purified anthocyanin; then a preparative liquid chromatography is used to separate and obtain petunia-3-O-glucoside; and finally, caffeic acid and petunidin-3-O-glucoside react, and dilution, stabilization by ultrahigh pressure, etc. are conducted to obtain the derivative. The derivative has better photothermal stability thanthe petunidin-3-O-glucoside, and has antioxidant and antitumor effects.

Description

technical field [0001] The invention belongs to the technical field of compounds and their preparation, and specifically relates to a stabilized petunidin-3-O-glucoside derivative and a preparation method thereof. Background technique [0002] Anthocyanins, also known as anthocyanins, are widely found in plants. Fruits, vegetables, flowers, and grains are rich in anthocyanins, such as blueberries, grapes, blueberries, lingonberries, mulberries, strawberries, purple sweet potatoes, purple Corn, purple cabbage, black beans, black rice, roselle, etc. Anthocyanins are high in grapes, blueberries, and indigo fruits, especially mountain grapes, which can be used as a good source of natural anthocyanins. Anthocyanins belong to flavonoid compounds, among which common anthocyanins include geranium, cyanidin, delphinidin, paeoniflorin, petunienin, mallowin and so on. As a powerful natural antioxidant, anthocyanins are not only consistent with the free radical scavenging mechanism of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H1/08C07H1/00C07H17/065A61P35/00A61P35/02A61P39/06
CPCA61P35/00A61P35/02A61P39/06C07H1/00C07H1/08C07H17/065
Inventor 郑飞越皓陈长宝文连奎贺阳
Owner CHANGCHUN UNIV OF CHINESE MEDICINE
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