Fungal spore separation device based on microporous filter films, system applying fungal spore separation device, and separation method applied to system

Abstract

The invention provides a fungal spore separation device based on microporous filter films, a system applying the fungal spore separation device, and a separation method applied to the system. The separation device comprises an upper-layer sample injection pool, an upper-layer silical gel rubber ring, an upper-layer microporous filter film, an upper-layer drainage tube, an upper-layer clamping clamp, a lower-layer sample injection pool, a lower-layer silical gel rubber ring, a lower-layer microporous filter film, a lower-layer drainage tube and a lower-layer clamping clamp which are sequentially connected from top to bottom; the upper-layer sample injection pool and the lower-layer sample injection pool are each a hollow cylinder; an upper-layer sample injection opening and a lower-layer sample injection opening are formed in the bottom of the upper-layer sample injection pool and the bottom of the lower-layer sample injection pool correspondingly; the sample injection pools and the drainage tubes are closely connected through the clamping clamps; and the lower end of the upper-layer drainage tube sequentially penetrates through the middle of the upper-layer clamping clamp and penetrates into the lower-layer sample injection pool. The double layers of microporous filter films have the great flexibility, can be taken down after filtering, are directly folded to be placed into anEP tube for follow-up detection, and are highly compatible with a downstream detection technology. Different targets are selectively separated and enriched mainly through the physical property difference between fungal spore and other cells in body fluid.

Description

technical field [0001] This application relates to the fields of advanced manufacturing and biomedical technology, in particular to a fungal spore separation device based on the difference in physical properties (such as size and deformability) between fungal spores and background cells, as well as its system and separation method. Background technique [0002] Globally, invasive fungal diseases cause 11.5 million serious infections and 1.5 million deaths every year, and have become one of the important diseases that seriously threaten people's lives. But so far, there is still no rapid and accurate diagnostic method for invasive fungal diseases that cause serious consequences, especially fungemia after fungi enter the blood. Fungi may also be present in other bodily fluids such as urine and sputum. [0003] Take candidemia, the most common form of fungemia, as an example. Two days after infection, the mortality rate is >40%. However, if treated on the same day, the mort...

AI Technical Summary

Problems solved by technology

Due to the low pathogen load in the blood, the diagnosis of fungemia still depends on blood culture, which takes a long time to diagnose, only culture needs 3-7 days, and the positive rate is less than 10%

[0005] However, limited by factors such as small chip size and large flow resistance, micro-nanofluidic chips have low throughput and time-consuming volume operations, and cannot operate large-volume samples. Therefore, it is difficult to solve the problem of low loads of fungal spores in body fluids. There is still a long way to go before clinical application

Images