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A culture medium for human peripheral blood lymphocytes
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A technology of lymphocytes and human peripheral blood, applied in the field of cell culture medium, which can solve the problems of complex components and difficult control, and achieve good culture effect and good culture effect
Active Publication Date: 2022-03-29
HANGZHOU GENE META MEDICAL DEVICE CO LTD
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[0010] Aiming at the problem that the existing cell culture medium contains serum, protein and other biological extracts, resulting in complicated and difficult-to-control components, the present invention provides a culture medium for human peripheral blood lymphocytes and a preparation method thereof
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[0030] 2) Add 5% bovine serum of the total volume and mix well.
[0031] 3) Add the following substances to make the final concentration meet the formulation requirements: gentamicinsulfate, heparin sodium, mercaptoethanol, glutamine, calcium ions, iron ions, titanium ions, and mix well.
[0032] 4) After mixing the above solution, filter it with a 0.22 μm filter membrane, then add the phytohemagglu...
[0037] RPMI 1640 medium 10.4g / L, sodium bicarbonate 2.0g / L, HEPES free acid 2.38g / L, gentamicin sulfate 50μg / mL, heparin sodium 6.9U / mL, mercaptoethanol 7.15μmol / L, glutamine Amide 0.25mmol / L, calcium ion 0.8mmol / L, iron ion 1.86μmol / L, titanium ion 20.8μmol / L, lectin 54mg / L.
[0038] The preparation method is similar to Example 1.
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Abstract
The invention belongs to the technical field of cell culture medium and discloses a human peripheralblood lymphocyte culture medium. The medium of the present invention mainly comprises the following components: RPMI 1640 medium powder, sodiumbicarbonate, HEPESfree acid, gentamicinsulfate, heparinsodium, mercaptoethanol, glutamine, calciumion, iron ion, titaniumion, plant blood Lectin. The culture medium of the present invention may contain serum or protein components, or may be free of serum and protein components, and it mainly utilizes certain specific metal ions to promote cell proliferation. The culture medium of the invention has clear components, stable performance and low cost, can effectively promote the proliferation and division of lymphoblasts in human whole blood, and has a relatively high lymphocyte transformation rate and lymphocyte division index.
Description
technical field [0001] The invention belongs to the technical field of cell culture medium, and in particular relates to a formula of human peripheralblood lymphocyte culture medium and a preparation method thereof. Background technique [0002] Lymphocytes in human peripheral blood are almost in the GI phase (G0 phase), and generally do not continue to divide. If conA, lipopolysaccharide (LPS), and interleukin When IL-2, phytohemagglutinin (PHA) and other mitogens and antigenic substances are released, small lymphocytes will be stimulated by the inducer to transform into lymphoblasts, and then enter mitosis. After short-term cell culture, colchicinesolution treatment, hypotonicity and fixation, some metaphase cells of mitosis can be obtained. This method has been widely used in clinical medicine, virology, pharmacology, genetic toxicity and so on. [0003] Causes known to affect the growth of human peripheral blood lymphocytes are: [0004] 1) Virus or microbial infect...
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