Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Electrochemical biosensor for detecting tetrabromobisphenol A bis (2-hydroxyethyl) ether, preparation method thereof and application thereof

A biosensor, hydroxyethyl technology, applied in electrochemical variables of materials, scientific instruments, instruments, etc., can solve the problem of strong neurotoxicity and cytotoxicity, inability to detect tetrabromobisphenol A bis(2-hydroxyethyl) ether, etc. problem, to achieve the effect of low cost, improved electrochemical performance, and convenient operation

Active Publication Date: 2020-01-10
佛山市南海区里水镇经济促进局 +1
View PDF8 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Tetrabromobisphenol A bis(2-hydroxyethyl) ether (TBBPA DHEE) is a typical TBBPA derivative, which is widely used, but has potential neurotoxicity and the strongest cytotoxicity
However, tetrabromobisphenol A bis(2-hydroxyethyl) ether cannot be detected, so there is an urgent need to develop a new nanometer electrochemical biosensor that can detect tetrabromobisphenol A bis(2-hydroxyethyl) ether. (TBBPADHEE) for rapid and accurate detection

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A method for preparing an electrochemical biosensor for detecting tetrabromobisphenol A bis(2-hydroxyethyl) ether, comprising the following steps:

[0030] (1) Take 1.6g of graphite powder and 0.8g of N-hexylpyridinium hexafluorophosphate (HPPF6) and grind them in a mortar for 3h to obtain carbon paste, then fill the carbon paste into a glass electrode tube with an inner diameter of 4mm and compact it. A copper wire was inserted as a wire to obtain a carbon ionic liquid electrode CILE.

[0031] (2) Modification of ZIF-8: 6 μL of 0.4 mg / mL ZIF-8 suspension was drop-coated on the surface of CILE obtained in the step (2) to obtain ZIF-8 modified CILE, namely ZIF-8 / CILE.

[0032] (3) Electrodeposition of Cl 2 -MWNTs: the ZIF-8 / CILE electrode obtained in the step (2) was soaked in 1mg / mL Cl 2-In the MWNTs solution, using cyclic voltammetry, the initial voltage and high potential are set at 0.5V, the low potential is -1.5V, and the electrodeposited Cl is cyclically scanned ...

Embodiment 2

[0037] A method for preparing an electrochemical biosensor for detecting tetrabromobisphenol A bis(2-hydroxyethyl) ether, comprising the following steps:

[0038] (1) Take 1.6g of graphite powder and 0.8g of N-hexylpyridinium hexafluorophosphate (HPPF6) and grind them in a mortar for 3h to obtain carbon paste, then fill the carbon paste into a glass electrode tube with an inner diameter of 4mm and compact it. A copper wire was inserted as a wire to obtain a carbon ionic liquid electrode CILE.

[0039] (2) Modification of ZIF-8: 4 μL of 0.6 mg / mL ZIF-8 suspension was drop-coated on the surface of CILE obtained in the step (2) to obtain ZIF-8 modified CILE, namely ZIF-8 / CILE.

[0040] (3) Electrodeposition of Cl 2 -MWNTs: the ZIF-8 / CILE electrode obtained in the step (2) was soaked in 1mg / mL Cl 2 -In the MWNTs solution, using cyclic voltammetry, the initial voltage and high potential are set at 0.5V, the low potential is -1.5V, and the electrodeposited Cl is cyclically scanned...

Embodiment 3

[0044] A method for preparing an electrochemical biosensor for detecting tetrabromobisphenol A bis(2-hydroxyethyl) ether, comprising the following steps:

[0045] (1) Take 1.6g of graphite powder and 0.8g of N-hexylpyridinium hexafluorophosphate (HPPF6) and grind them in a mortar for 3h to obtain carbon paste, then fill the carbon paste into a glass electrode tube with an inner diameter of 4mm and compact it. A copper wire was inserted as a wire to obtain a carbon ionic liquid electrode CILE.

[0046] (2) Modification of ZIF-8: 8 μL of 0.2 mg / mL ZIF-8 suspension was drop-coated on the surface of CILE obtained in the step (2) to obtain ZIF-8 modified CILE, namely ZIF-8 / CILE.

[0047] (3) Electrodeposition of Cl 2 -MWNTs: the ZIF-8 / CILE electrode obtained in the step (2) was soaked in 1mg / mL Cl 2 -In the MWNTs solution, using cyclic voltammetry, the initial voltage and high potential are set at 0.5V, the low potential is -1.5V, and the electrodeposited Cl is cyclically scanned...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an electrochemical biosensor for detecting tetrabromobisphenol A bis (2-hydroxyethyl) ether, a preparation method thereof and application thereof. According to the electrochemical biosensor, a carbon ionic liquid electrode is used as a substrate electrode, and a surface of the substrate electrode is sequentially modified with a zinc metal organic framework nano material layer, a chlorine-doped multi-walled carbon nanotube layer, a monoclonal antibody layer and a Nafion film. The preparation method of the electrochemical biosensor comprises the following steps of firstlymodifying ZIF8 on the surface of the CILE, then electrodepositing Cl2MWNTs, assembling a monoclonal antibody, and finally covering with the Nafion film. The nano electrochemical biosensor for detecting tetrabromobisphenol A bis (2-hydroxyethyl) ether has a wide detection range and low detection limit and can be used for detecting a sample containing the tetrabromobisphenol A bis (2-hydroxyethyl)ether.

Description

technical field [0001] The present invention relates to the technical fields of nanomaterials, electrochemical detection and biosensors, more specifically, to an electrochemical biosensor for detecting tetrabromobisphenol A bis(2-hydroxyethyl) ether and its preparation method and application. Background technique [0002] Electrochemical biosensors refer to sensors that use immobilized biological materials as recognition elements. Its working principle is to use biological components (enzymes, polypeptides, proteins, antibodies, antigens, etc.) or organisms themselves (cells, tissues, organelles, etc.) The conversion element, through the specific recognition between biomolecules, selectively recognizes the target molecule and captures the target molecule on the surface of the electrode, and then derives the signal from the basic electrode, using potential, current, capacitance or conductance as the characteristic detection signal sensor. Electrochemical biosensors occupy ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N27/327G01N27/30G01N33/577G01N27/48
CPCG01N27/308G01N27/327G01N27/48G01N33/577
Inventor 曾晖李国滨
Owner 佛山市南海区里水镇经济促进局
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com