Culture medium for screening endophytic fungi of cypripedium macranthum

An endophytic fungus and culture medium technology, applied in the field of microorganisms, can solve the problems of low transplant survival rate, slow growth and development, late flowering, etc., and achieve the effects of improving broad-spectrum, improving metabolic activity, and promoting mycelial growth.

Active Publication Date: 2020-01-14
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Cypripedium grandiflora has excellent ornamental and medicinal properties, however, under natural conditions, the reproduction rate of Cypripedium grandiflora is extremely low, while tissue culture Due to the lack of symbiosis with the corresponding mycorrhizal fungi, Cypripedium grandiflora not only has a low survival rate of transplanting, slow growth and development, but also blooms late or even does not bloom. Therefore, the screening of mycorrhizal fungi for Cypripedium grandiflorum is extremely important

Method used

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  • Culture medium for screening endophytic fungi of cypripedium macranthum
  • Culture medium for screening endophytic fungi of cypripedium macranthum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A culture medium for screening endophytic fungi of Cypripedium grandiflora, made of the following components in parts by weight: 45% potato, 200% guava juice, 18% glucose, 15% ammonium sulfate, 0.2% magnesium sulfate, 0.15% potassium alginate, vitamin 0.15, agar 20, 5-bromo-4-chloro-3-indole-α-D-galactoside 0.01, corn silk extract 35, which also includes streptomycin with a concentration of 100 μg / g, and the pH of the medium is 6.5;

[0019] Wherein, guava juice preparation method is:

[0020] Peel the guava, then add it to the wall-breaking machine, add 5 times the water of the guava, carry out the wall-breaking treatment for 20 minutes, then filter, remove the large particle filter residue, obtain the filtrate, and sterilize the filtrate. The guava juice was obtained, and the sterilization was performed by high-pressure steam sterilization at 132°C.

[0021] Wherein, the mixing weight ratio of potassium alginate and vitamin is 1:1.

[0022] Among them, the vitamin ...

Embodiment 2

[0027] A culture medium for screening endophytic fungi of Cypripedium grandiflora, made of the following components in parts by weight: potato 50, guava juice 250, glucose 22, ammonium sulfate 19, magnesium sulfate 0.4, potassium alginate 0.19, vitamin 0.19, agar 25, 5-bromo-4-chloro-3-indole-α-D-galactoside 0.02, corn silk extract 40, which also includes streptomycin with a concentration of 100 μg / g, and the pH of the medium is 6.6;

[0028] Wherein, guava juice preparation method is:

[0029] Peel the guava, then add it to the wall-breaking machine, add 5 times the water of the guava, carry out the wall-breaking treatment for 20 minutes, then filter, remove the large particle filter residue, obtain the filtrate, and sterilize the filtrate. The guava juice was obtained, and the sterilization was performed by high-pressure steam sterilization at 132°C.

[0030] Wherein, the mixing weight ratio of potassium alginate and vitamin is 1:1.

[0031] Among them, the vitamin is vit...

Embodiment 3

[0036] A culture medium for screening endophytic fungi of Cypripedium grandiflora, made of the following components in parts by weight: 55% potato, 220% guava juice, 19% glucose, 18% ammonium sulfate, 0.3% magnesium sulfate, 0.17% potassium alginate, vitamin 0.17, agar 22, 5-bromo-4-chloro-3-indole-α-D-galactoside 0.015, corn silk extract 38, which also includes streptomycin with a concentration of 100 μg / g, and the pH of the medium is 6.8;

[0037] Wherein, guava juice preparation method is:

[0038] Peel the guava, then add it to the wall-breaking machine, add 5 times the water of the guava, carry out the wall-breaking treatment for 20 minutes, then filter, remove the large particle filter residue, obtain the filtrate, and sterilize the filtrate. The guava juice was obtained, and the sterilization was performed by high-pressure steam sterilization at 121°C.

[0039] Wherein, the mixing weight ratio of potassium alginate and vitamin is 1:1.

[0040] Among them, the vitamin...

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Abstract

The invention discloses a culture medium for screening endophytic fungi of cypripedium macranthum. The culture medium is prepared from the following components including potatoes, a guava juice, glucose, ammonium sulfate, magnesium sulfate, potassium alginate, vitamins, agar, 5-bromine-4-chlorine-3-indole-alpha-D-galactoside, a corn stigma leaching solution and streptomycin, wherein a pH value ofthe culture medium was 6.5-6.8. For the culture medium for separating mycorrhiza fungi of the cypripedium macranthum, through a large number of experimental researches, careful preparation of a culture medium formula aims at further improving the effectiveness of separation and purification of the mycorrhiza fungi of the cypripedium macranthum; and in the formula, by specially adding two core components which are the 5-bromine-4-chlorine-3-indole-alpha-D-galactoside and the corn stigma leaching solution, the culture medium can have the effect of promoting growth of mycelia of the mycorrhiza fungi of the cypripedium macranthum.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a culture medium for screening endophytic fungi of Cypripedium grandiflora. Background technique [0002] The plant height of Cypripedium grandiflora is 25-50cm, with thick and short rhizomes. Stem erect, slightly pubescent or glabrous, with several sheaths at the base and 3-4 leaves above the sheaths. Leaf blade elliptic or elliptic-ovate, 10-15cm long, 6-8cm wide, apex acuminate or subacute, slightly pubescent or glabrous on both veins, with ciliates at the margin. Inflorescence terminal, with 1 flower, rarely 2 flowers; inflorescence peduncle pubescent or glabrous; bracts leaflike, usually elliptic, less elliptic-lanceolate, 7-9cm long, 4-6cm wide , apex short and acuminate, usually puberulent on both veins; pedicel and ovary 3-3.5cm long, glabrous; flowers large, purple, red or pink, usually with dark veins, rarely white. [0003] Cypripedium grandifloru...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/38C12N1/14
CPCC12N1/38C12N1/14
Inventor 陈丽飞胡良军李家琦陈翠红孟缘姜大崴
Owner JILIN AGRICULTURAL UNIV
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