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A kind of preparation method of cysteamine synergistic gold-silver nano material

A technology of nanomaterials and cysteamine, which can be used in medical preparations with non-active ingredients, wave energy or particle radiation treatment materials, medical preparations containing active ingredients, etc., and can solve the problems of reduced bactericidal effect and antibacterial activity , to achieve the effect of enhanced bactericidal effect, good stability and good dispersibility

Active Publication Date: 2021-06-25
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, pure nano-silver materials are easy to aggregate, resulting in reduced antibacterial activity.
Coating a layer of gold material on the surface of silver nanoparticles can stabilize gold nanomaterials, however, it inhibits the release of silver ions and reduces the bactericidal effect

Method used

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  • A kind of preparation method of cysteamine synergistic gold-silver nano material
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  • A kind of preparation method of cysteamine synergistic gold-silver nano material

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Embodiment 1

[0030] Embodiment 1: the preparation method of material of the present invention, comprises the steps:

[0031] (1) Preparation of template silver nanocubes of gold and silver nanocages: 0.192 g of silver nitrate, 0.11 g of sodium hydrosulfide, and 0.1 g of polyvinylpyrrolidone were weighed and dissolved in 4 mL, 5 mL, and 5 mL of ethylene glycol, respectively. After the dissolution is complete, immediately freeze the silver nitrate solution and sodium hydrosulfide solution at -20°C for at least half an hour for later use. Heat the ethylene glycol solution to 150°C in an oil bath, and blow in nitrogen gas while heating (100mL min -1 ), while stirring with a magnet at a speed of 1000rmin -1 After 30 minutes, 2.5mL polyvinylpyrrolidone solution and 120ul sodium hydrosulfide solution were added successively. Continue heating and stirring. After 30 minutes, close the nitrogen valve, pull out the catheter leading into nitrogen, and slowly add 850ul of silver nitrate solution. C...

Embodiment 2

[0034] Embodiment 2: Cysteamine induces aggregation of gold and silver nanocages

[0035] Using phosphate buffer as a solvent, a cysteamine solution with a concentration of 10 mM was prepared for use. Dilute the gold-silver nanocages to 32 µg / mL with deionized water. Mix the two at a volume ratio of 1:1, and record their UV-visible absorption spectra every 6 minutes for 1 hour. The obtained results are as figure 2 Shown: within 1h, the absorption peak in the visible light range begins to weaken, and the absorption peaks in the near-infrared and near-infrared regions gradually increase.

Embodiment 3

[0036] Embodiment 3: Cysteamine induces gold-silver nanocages to release silver particles

[0037] With the same amount of gold and silver nanocages in Example 2, mix with 10mM cysteamine, L-cysteine, glutathione, N-acetyl-L-cysteine ​​solution (pH=7) respectively for 4 Hour. In the control group, only an equal amount of phosphate buffer was added and mixed. Afterwards, separate the mixed solution with an ultrafiltration tube with a molecular weight cut-off of 10kDa, the centrifugation speed is 5000 rpm, and the time is 5 minutes, and the lower layer part is measured by inductively coupled plasma mass spectrometry to measure the silver content therein. From image 3 It can be seen that cysteamine can significantly induce the release of silver particles and has good antibacterial potential.

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Abstract

The invention discloses a method for preparing cysteamine synergistic gold and silver nanomaterials. Firstly, silver nano cubes are mixed with an acetone solution of equal volume, centrifuged, and ethanol aqueous solution is added to the precipitate to completely dissolve, centrifuged again, and ethanol aqueous solution is used to Washing, distilling the supernatant to volume with ethanol ultrapure water to obtain a silver nanocube solution for subsequent use; take an aqueous solution of polyvinylpyrrolidone, add the silver nanocube solution in a water bath environment, stir, then add dropwise an aqueous solution of chloroauric acid , when the solution turns purple, stop the dropwise addition, cool to room temperature, add excess sodium chloride, stir, collect the supernatant, wash with ethanol aqueous solution, and dilute the supernatant with ethanol ultrapure water; use deionized After diluting with water, add cysteamine solution and mix well. The invention introduces cysteamine and the strong interaction of the gold-sulfur bond to induce the gold-silver nano-material to release silver ions rapidly, thereby producing a strong bactericidal effect, and the introduction of infrared laser can enhance the bactericidal effect.

Description

technical field [0001] The invention relates to a preparation method of an antibacterial nanometer material, in particular to a preparation method of a cysteamine synergistic gold and silver nanometer material. Background technique [0002] As an ancient antibacterial, silver is widely used in all aspects of life. The development of nanotechnology has enabled silver to be used as an antibacterial agent on a new scale. However, pure nano-silver materials are easy to aggregate, resulting in reduced antibacterial activity. Coating a layer of gold material on the surface of silver nanoparticles can stabilize the gold nanomaterials, however, it inhibits the release of silver ions and reduces the bactericidal effect. Contents of the invention [0003] Purpose of the invention: The purpose of the invention is to provide a method for preparing cysteamine synergistic gold and silver nanomaterials. [0004] Technical solution: a method for preparing cysteamine synergistic gold an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K33/38A61K41/00A61K9/51A61K47/20A61K47/02A61P31/04
CPCA61K9/5115A61K9/5123A61K33/38A61K41/0052A61P31/04A61K2300/00
Inventor 姜晖秦肇建杜天宇王仪涵张佳蕾陈芸王雪梅
Owner SOUTHEAST UNIV
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