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Extract for artificially cultured phellinus igniarius fruiting bodies as well as preparation method and application for extract

A technology of artificial cultivation and Phellinus linteus, applied in the fields of application, medical preparations containing active ingredients, food science, etc., to achieve the effects of improving side effects, improving cell survival rate, and reducing cell damage

Active Publication Date: 2020-01-17
淳安千岛湖桑都食用菌专业合作社 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Phellinus radicis fruiting body has various active components, the main medicinal components are flavonoids, polysaccharides, pyrones and triterpenoids, which have pharmacological functions such as anti-tumor, enhancing body immunity, anti-oxidation, anti-inflammation, lowering blood sugar and blood fat, and anti-pneumonia. There is no report on the treatment of gout and hyperuricemia

Method used

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  • Extract for artificially cultured phellinus igniarius fruiting bodies as well as preparation method and application for extract
  • Extract for artificially cultured phellinus igniarius fruiting bodies as well as preparation method and application for extract
  • Extract for artificially cultured phellinus igniarius fruiting bodies as well as preparation method and application for extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Preparation of artificially cultivated Phellinus Phellinus fruiting body extract

[0031] Take 200g of the Phellinus fruiting body, put it in a swing grinder, and pulverize it into medium powder, and mix evenly. Take 20g, put it in a round bottom flask, add 500mL of 70% ethanol aqueous solution by volume, soak for 0.5h, reflux extraction for 1h, filter, collect the filtrate, wash the filter residue with 100mL of 70% ethanol by volume, combine the filtrate, and place in a rotary evaporation The ethanol was recovered in the container until there was no alcohol smell, and vacuum-dried (60° C.) to obtain 5.12 g of Phellinus Phellinus fruiting body extract.

Embodiment 2

[0032] Example 2: The effect of artificially cultivated Phellinus radix fruiting body extract on cell survival rate induced by sodium urate

[0033] Human umbilical vein endothelial cells were cultured in a culture flask to the logarithmic growth phase, digested with trypsin, centrifuged, resuspended, counted, and prepared to a cell density of 8×10 4 cells / mL of cell suspension, 100 μL was inoculated on a 96-well culture plate, and placed in CO 2 Cultivate in the incubator for 24h. Afterwards, the culture plate was taken out, the old culture medium was discarded, and the culture medium was added according to the following groups: normal control group, sodium urate group (150 μg / mL), sodium urate + different doses of Phellinus fruiting body extract group (10,20,40 , 80, 160 and 320 μg / mL), each group set up 6 parallel wells, incubated in the incubator for 24h, 20μL of 5mg / mL MTT, continued to incubate for 4h. Discard the culture medium, add 150 μL DMSO, shake at room temperat...

Embodiment 3

[0035] Example 3: The uric acid-lowering effect of artificially cultivated Phellinus radix fruiting body extract on hyperuricemia mice induced by potassium oxonate

[0036] 60 male ICR mice (body weight 18-22g) were fed adaptively for one week, and randomly divided into 6 groups: normal control group, hyperuricemia model group, Phellinus fruiting body extract 450, 150 and 50mg / kg doses Allopurinol 10mg / kg in the control group and the positive control group. The test drug was made into an appropriate concentration with 0.5% carboxymethylcellulose sodium (0.5% CMC-Na), and each group was administered by intragastric administration at regular intervals every morning for 7 consecutive days. Mouse weighing records, results figure 2 shown. On the 7th day, except for the normal group, the other groups were intraperitoneally injected oxonic acid potassium salt 350mg / kg 0.5h before the last administration to form a hyperuricemia model in mice, and the normal control group was intrap...

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Abstract

The invention discloses an extract for artificially cultured phellinus igniarius fruiting bodies as well as a preparation method and application for the extract. The preparation method comprises the following steps of selecting Sangdu No. 1 as a phellinus igniarius strain, adopting a bagging cultivation method in imitating a wild condition under a forest, harvesting the fruiting bodies when the color of the fruiting bodies changes from yellow to brown or dark brown, and obtaining the phellinus igniarius fruiting bodies; and crushing the phellinus igniarius fruiting bodies into powder, soakingthe powder with an ethanol aqueous solution, performing reflux extraction, performing vacuum concentration on an extracting solution until an ethanol smell does not exist, and performing vacuum dryingto obtain the extract for the artificially cultured phellinus igniarius fruiting bodies. The extract disclosed by the invention is capable of improving and relieving kidney injury of a mouse with hyperuricemia and increasing uric acid excretion, is moderate in effect and less in side effects and can be used for preparing the drug and the health-care product for preventing and treating the hyperuricemia and gout.

Description

technical field [0001] The invention relates to the field of the extract of the fruiting body of Phellinus radix, in particular to an extract of the artificially cultivated fruiting body of Phellinus phellidis and its preparation method and its new application in the prevention and treatment of hyperuricemia and gout. Background technique [0002] Gout belongs to the category of metabolic diseases. It is a series of inflammatory reactions caused by the deposition of urate due to the metabolic disorder of purine substances in the organism or the reduction of uric acid excretion in the kidneys. When the uric acid in the serum rises to a supersaturated state, the extracellular fluid is deposited in the joints, tissues and organs around the joints, causing a group of gout syndrome (including tophi, uric acid kidney stones, gouty arthritis and gouty nephropathy) . According to statistics, the overall incidence of gout in the world is 0.06‰–2.68‰. In my country, gout has become ...

Claims

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Application Information

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IPC IPC(8): A61K36/07A61P19/06A23L33/105
CPCA61K36/07A61P19/06A23L33/105A61K2236/333A61K2236/39A61K2236/53A61K2236/51A23V2002/00A23V2200/30A23V2250/208
Inventor 张信岳王建功李钦吕玮谷丽丽王星晨武柠子王建文
Owner 淳安千岛湖桑都食用菌专业合作社
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