Preparation method of silk fibroin lyophilized powder

Abstract

The invention provides a preparation method of silk fibroin lyophilized powder. The preparation method includes that small molecular silk fibroin is formed after silk fibroin is enzymatically hydrolyzed, starch is introduced and enzymatically hydrolyzed, viscous small molecular substance is generated after starch enzymolysis and adhered to the surface of the small molecular silk fibroin, and induction action of alcohol, crosslinking action of polyethylene glycol diglycidyl ether and freeze-thawing action are combined to enable the silk fibroin lyophilized powder to be in nanoscale size, supportive of transdermal absorption and good in cosmetic effect. The silk fibroin lyophilized powder is a lyophilized powder product and is high in activity and convenient to use.

Description

technical field

[0001] The invention relates to the technical field of beauty products, in particular to a preparation process of silk fibroin freeze-dried powder. Background technique

[0002] Human skin is exposed to ultraviolet rays, smog and other damage from the outside world, and with the continuous deterioration of human living environment and the acceleration of the pace of life, environmental pollution and life pressure will bring many problems to human skin, such as: dry skin, Spots, acne, wrinkles, sensitive skin, etc. In addition, since people's demand for quality of life has also been greatly improved compared with the past, the demand for beauty care is also increasing. With the formation of the concept of overall health and the pursuit of a higher quality of life, the development of China's beauty industry has increased rapidly in recent years, with an annual growth rate of more than 20%. It has become the third largest market in the world, and the industry h...

Examples

Embodiment 1

[0039] A preparation process of silk fibroin freeze-dried powder, the specific steps are as follows:

[0040] (1) first extract silk fibroin to obtain a silk fibroin solution;

[0041] (2) Then add elastase to realize the enzymolysis of silk fibroin, add starch, ultrasonically oscillate evenly, then add β-amylase and pullulanase to realize the enzymolysis of starch, filter to obtain a mixed solution;

[0042] (3) Then slowly drop the mixed solution into ethanol while stirring, add polyethylene glycol diglycidyl ether after the dropwise addition, and react at 50°C for 80 minutes;

[0043] (4) freeze-thaw treatment, sterilization, freeze-drying, and packaging to obtain the above-mentioned freeze-dried silk fibroin powder.

[0044] Wherein, the specific method of the step (1) is: first degumming the silk, and then hydrolyzing it to obtain the silk fibroin solution. The silk is mulberry silk. The specific method of degumming treatment is as follows: soak silk in 0.1% sodium car...

Embodiment 2

[0054] A preparation process of silk fibroin freeze-dried powder, the specific steps are as follows:

[0055] (1) first extract silk fibroin to obtain a silk fibroin solution;

[0056] (2) Then add elastase to realize the enzymolysis of silk fibroin, add starch, ultrasonically oscillate evenly, then add β-amylase and pullulanase to realize the enzymolysis of starch, filter to obtain a mixed solution;

[0057] (3) Slowly add the mixed solution into ethanol dropwise while stirring, add polyethylene glycol diglycidyl ether after the dropwise addition, and react at 55°C for 70 minutes;

[0058] (4) freeze-thaw treatment, sterilization, freeze-drying, and packaging to obtain the above-mentioned freeze-dried silk fibroin powder.

[0059] Wherein, the specific method of the step (1) is: first degumming the silk, and then hydrolyzing it to obtain the silk fibroin solution. The silk is tussah silk. The specific method of degumming treatment is: soak silk in 0.05% sodium carbonate aq...

Embodiment 3

[0069] A preparation process of silk fibroin freeze-dried powder, the specific steps are as follows:

[0070] (1) first extract silk fibroin to obtain a silk fibroin solution;

[0071] (2) Then add elastase to realize the enzymolysis of silk fibroin, add starch, ultrasonically oscillate evenly, then add β-amylase and pullulanase to realize the enzymolysis of starch, filter to obtain a mixed solution;

[0072] (3) Slowly add the mixed solution into ethanol dropwise while stirring, add polyethylene glycol diglycidyl ether after the dropwise addition, and react at 53°C for 75 minutes;

[0073] (4) freeze-thaw treatment, sterilization, freeze-drying, and packaging to obtain the above-mentioned freeze-dried silk fibroin powder.

[0074] Wherein, the specific method of the step (1) is: first degumming the silk, and then hydrolyzing it to obtain the silk fibroin solution. The silk is celestial silk. The specific method of degumming treatment is: immerse silk in 0.08% sodium carbon...

technical field

[0001] The invention relates to the technical field of beauty products, in particular to a preparation process of silk fibroin freeze-dried powder. Background technique

[0002] Human skin is exposed to ultraviolet rays, smog and other damage from the outside world, and with the continuous deterioration of human living environment and the acceleration of the pace of life, environmental pollution and life pressure will bring many problems to human skin, such as: dry skin, Spots, acne, wrinkles, sensitive skin, etc. In addition, since people's demand for quality of life has also been greatly improved compared with the past, the demand for beauty care is also increasing. With the formation of the concept of overall health and the pursuit of a higher quality of life, the development of China's beauty industry has increased rapidly in recent years, with an annual growth rate of more than 20%. It has become the third largest market in the world, and the industry h...

Examples

Embodiment 1

[0039] A preparation process of silk fibroin freeze-dried powder, the specific steps are as follows:

[0040] (1) first extract silk fibroin to obtain a silk fibroin solution;

[0041] (2) Then add elastase to realize the enzymolysis of silk fibroin, add starch, ultrasonically oscillate evenly, then add β-amylase and pullulanase to realize the enzymolysis of starch, filter to obtain a mixed solution;

[0042] (3) Then slowly drop the mixed solution into ethanol while stirring, add polyethylene glycol diglycidyl ether after the dropwise addition, and react at 50°C for 80 minutes;

[0043] (4) freeze-thaw treatment, sterilization, freeze-drying, and packaging to obtain the above-mentioned freeze-dried silk fibroin powder.

[0044] Wherein, the specific method of the step (1) is: first degumming the silk, and then hydrolyzing it to obtain the silk fibroin solution. The silk is mulberry silk. The specific method of degumming treatment is as follows: soak silk in 0.1% sodium car...

Embodiment 2

[0054] A preparation process of silk fibroin freeze-dried powder, the specific steps are as follows:

[0055] (1) first extract silk fibroin to obtain a silk fibroin solution;

[0056] (2) Then add elastase to realize the enzymolysis of silk fibroin, add starch, ultrasonically oscillate evenly, then add β-amylase and pullulanase to realize the enzymolysis of starch, filter to obtain a mixed solution;

[0057] (3) Slowly add the mixed solution into ethanol dropwise while stirring, add polyethylene glycol diglycidyl ether after the dropwise addition, and react at 55°C for 70 minutes;

[0058] (4) freeze-thaw treatment, sterilization, freeze-drying, and packaging to obtain the above-mentioned freeze-dried silk fibroin powder.

[0059] Wherein, the specific method of the step (1) is: first degumming the silk, and then hydrolyzing it to obtain the silk fibroin solution. The silk is tussah silk. The specific method of degumming treatment is: soak silk in 0.05% sodium carbonate aq...

Embodiment 3

[0069] A preparation process of silk fibroin freeze-dried powder, the specific steps are as follows:

[0070] (1) first extract silk fibroin to obtain a silk fibroin solution;

[0071] (2) Then add elastase to realize the enzymolysis of silk fibroin, add starch, ultrasonically oscillate evenly, then add β-amylase and pullulanase to realize the enzymolysis of starch, filter to obtain a mixed solution;

[0072] (3) Slowly add the mixed solution into ethanol dropwise while stirring, add polyethylene glycol diglycidyl ether after the dropwise addition, and react at 53°C for 75 minutes;

[0073] (4) freeze-thaw treatment, sterilization, freeze-drying, and packaging to obtain the above-mentioned freeze-dried silk fibroin powder.

[0074] Wherein, the specific method of the step (1) is: first degumming the silk, and then hydrolyzing it to obtain the silk fibroin solution. The silk is celestial silk. The specific method of degumming treatment is: immerse silk in 0.08% sodium carbon...

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