Unlock instant, AI-driven research and patent intelligence for your innovation.

Kit for simultaneously detecting four dengue virus subtypes

A technology of dengue virus and dengue virus, which is applied in the field of kits for simultaneous detection of 4 types of dengue virus typing, can solve the problems of long time and low specificity of dengue virus typing detection

Pending Publication Date: 2020-01-21
SHENZHEN CENT FOR DISEASE CONTROL & PREVENTION
View PDF11 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a kit for simultaneous detection of 4 kinds of dengue virus typing, aiming to solve the problems of low specificity and long time in the detection of dengue virus typing in the prior art

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Kit for simultaneously detecting four dengue virus subtypes
  • Kit for simultaneously detecting four dengue virus subtypes
  • Kit for simultaneously detecting four dengue virus subtypes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] S01. Select and collect 1 strain each of yellow fever virus, Rift Valley fever virus, West Nile virus, Zika virus and chikungunya virus, 12 strains of dengue virus type I, 3 strains of dengue virus type II, dengue virus 3 strains of virus type III and 3 strains of dengue virus type IV samples; according to the method of the virus RNA extraction kit QIAamp Viral RNA Kit, the nucleic acid was extracted from the samples for later use; samples of dengue virus types I, II, III and IV were taken respectively Nucleic acid is used for positive sample verification; the nucleic acid of yellow fever virus sample, Rift Valley fever virus sample nucleic acid, West Nile virus sample nucleic acid, Zika virus sample nucleic acid, and Chikungunya virus sample nucleic acid are taken for specific detection;

[0068] S02. According to the known complete genome sequences of dengue virus type I, type II, type III and type IV, respectively design and synthesize dengue virus type I upstream spe...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a kit for simultaneously detecting four dengue virus subtypes. The kit comprises the following reagent: an RT-PCR (reverse transcription-polymerase chain reaction) solution, anRT-PCR enzyme, a positive quality control product and a negative quality control product, wherein the RT-PCR solution contains a one-step reaction RT-qPCR buffer, a dengue virus type-I upstream specific primer, a dengue virus type-I downstream specific primer, a dengue virus type-I blocking primer and a dengue virus type-I probe, a dengue virus type-II upstream specific primer, a dengue virus type-II downstream specific primer, a dengue virus type-II blocking primer and a dengue virus type-II probe, a dengue virus type-III upstream specific primer, a dengue virus type-III downstream specific primer, a dengue virus type-III blocking primer and a dengue virus type-III probe, and a dengue virus type-IV upstream specific primer, a dengue virus type-IV downstream specific primer, a dengue virustype-IV blocking primer and a dengue virus type-IV probe. The kit has the advantages of strong specificity and short detection time.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a kit for simultaneously detecting four types of dengue fever viruses. Background technique [0002] Dengue is transmitted by the bite of an Aedes mosquito infected with any of the four dengue viruses. The disease occurs in tropical and subtropical regions of the world. Symptoms appear 3-14 days after being bitten by an infectious mosquito. Symptoms range from mild fever to disabling high fever with severe headache, pain behind the eyeball, muscle and joint pain, and rash. There are no specific antiviral drugs for dengue fever. Dengue virus (Dengue virus) belongs to the Flaviviridae Flavivirus genus, is a single positive-sense RNA virus, and is divided into four serotypes according to the antigenicity of the envelope protein E. At present, the diagnostic methods for dengue virus at home and abroad mainly include serological test, virus culture and PCR, among which serological test ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/70C12Q1/686C12N15/11C12R1/93
CPCC12Q1/701C12Q1/686C12Q2600/16C12Q2537/143C12Q2561/101C12Q2563/107Y02A50/30
Inventor 张仁利郭永超陈莹莹阳帆黄亚兰黄达娜
Owner SHENZHEN CENT FOR DISEASE CONTROL & PREVENTION