A plasma protein isolate for treating Alzheimer's disease and its preparation method and application

A technology for Alzheimer's disease and plasma protein, applied in the field of molecular biology, can solve the problems that plasma protein isolates have not been reported, and achieve the effects of improving cognition, reducing drug dependence, and simple preparation methods

Active Publication Date: 2021-10-26
北京豪思生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the low-temperature ethanol separation method and the obtained plasma protein isolate specifically for Alzheimer's disease have not yet been reported.

Method used

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  • A plasma protein isolate for treating Alzheimer's disease and its preparation method and application
  • A plasma protein isolate for treating Alzheimer's disease and its preparation method and application
  • A plasma protein isolate for treating Alzheimer's disease and its preparation method and application

Examples

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preparation example Construction

[0036] In the second aspect, the present invention provides a method for preparing the above-mentioned plasma protein isolate, comprising the following steps: separating plasma, removing cryoprecipitate, precipitating plasma components by low-temperature ethanol method, removing IgM by affinity chromatography, sterilizing and freeze-drying.

[0037] Step 1. Plasma separation: In the above plasma separation step, the blood of the donor is collected by using an anticoagulant tube, and then the supernatant is collected by centrifugation to obtain plasma; and the plasma is stored at less than or equal to minus 30°C within 6-8 hours Freezing into blocks, the centrifugal force of the centrifugation process is 500-1200g, the centrifugation time is 10-30 minutes, and the centrifugation temperature is 0-8°C; (preferably minus 40°C) is frozen into blocks to obtain fresh frozen plasma.

[0038] An anticoagulant has been added to the above-mentioned anticoagulant tube, and the mass volume ...

Embodiment 1

[0082] The present embodiment is the preparation method of plasma protein isolate, and this method comprises the following steps:

[0083] (1) Plasma collection: The blood donor is a healthy person aged 20-30, the blood is obtained from blood donation in the hospital, the blood collection tube is an anticoagulant tube (sodium citrate is added as an anticoagulant, the mass volume ratio of the anticoagulant to blood 1:9), during the blood collection process, shake the blood collection tube while bleeding to prevent blood coagulation; put the blood collection tube into the centrifuge, set the centrifugal force to 1200g, centrifuge for 15 minutes, and the centrifugation temperature is 4°C; then use a pipette The supernatant is carefully drawn into the liquid container, which is the collected human plasma; and the plasma is frozen at minus 40°C (preferably minus 40°C) within 6-8 hours to obtain fresh frozen plasma.

[0084] (2) Removal of cryoprecipitate: after thawing the above fr...

Embodiment 2- Embodiment 8

[0183] The operations of the steps (1), (2), (4), (5), and (6) of the preparation method of the plasma protein isolate of Examples 2-Example 6 are all the same as those in Example 1.

[0184] The operation of step (3) is basically the same as embodiment 1, the difference is:

[0185] 1): In the first to fourth low-temperature ethanol precipitation systems, the ethanol volume percentage, temperature, protein concentration, ionic strength, and pH value different from that of Example 1 were used in Examples 2-6, see the table below for details; The fifth low-temperature ethanol precipitation was not carried out.

[0186]

[0187]

[0188] 2): in embodiment 2-6, adopt respectively different from embodiment 1, for the stirring time of the first to the fourth low-temperature ethanol precipitation system, the 3rd precipitation adopts the standing time different from embodiment 1, detailed see table below;

[0189] the first time the second time the third time ...

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Abstract

The invention belongs to the field of molecular biology, and relates to a plasma protein isolate for treating Alzheimer's disease, a preparation method and application thereof. The plasma protein isolate includes: α, β globulin, ceruloplasmin, transferrin, haptoglobin and albumin; the preparation method of the plasma protein isolate comprises in turn: separating plasma from blood, and performing The cryoprecipitate is removed, and the supernatant obtained by the cryoprecipitate removal is subjected to four low-temperature ethanol precipitations, and the precipitate obtained after the fourth low-temperature ethanol precipitation is used as the plasma protein isolate. In the present invention, through a series of reasonable operation steps, reasonable reagents and parameters are selected in each step, and various factors cooperate with each other to improve the curative effect of the plasma protein isolate on Alzheimer's disease and enhance memory. The preparation method of the invention is simple, can be directly scaled up for industrialized production, and can be applied to the pharmaceutical industry on a large scale.

Description

technical field [0001] The invention belongs to the field of molecular biology and relates to a plasma protein isolate, in particular to a plasma protein isolate for treating Alzheimer's disease and its preparation method and application. Background technique [0002] Alzheimer's disease (Alzheimer's disease, AD), also known as Alzheimer's disease, is a degenerative disease of the brain and central nervous system caused by the loss of neurons. The incidence of the disease in the elderly population is about 4%-8%. . In 1906, the German neuropathologist Alzheimer first reported a 51-year-old female patient with progressive memory loss. In 1910, psychiatrist Emil Kraepelin, known for naming and categorizing brain disorders, proposed the name Alzheimer's disease. The common clinical manifestations of the disease are progressive memory and cognitive decline, language barriers, psychomotor abnormalities, etc., which seriously affect the normal life of patients and bring heavy bu...

Claims

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Application Information

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IPC IPC(8): C07K1/36C07K1/34C07K1/22A61K38/41A61P25/28
CPCA61K38/17A61K38/1709A61K38/38A61K38/41A61P25/28C07K1/22C07K1/34C07K1/36A61K2300/00
Inventor 栗琳崔雅轩江振作陈玥管书丽石浩威丁亮
Owner 北京豪思生物科技股份有限公司
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