Fermented birch juice and production method thereof

A birch sap and fermentation product technology, applied in the field of fermented birch sap and its production, can solve the problems of restricting the application of fermented birch sap, increasing the energy consumption of processing, and not having other active ingredients in birch sap, and achieving excellent Inhibition rate of tyrosinase activity and antioxidant activity, improvement of raw material utilization rate, and effect of good skin topical efficacy

Active Publication Date: 2020-01-31
NATURAL MEDICINE INST OF ZHEJIANG YANGSHENGTANG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the technological purpose of existing fermented birch sap all is to increase the ethanol or acetic acid content in the birch sap, can not make the birch sap have other active components, thus limits the application of fermented birch sap
CN109077960A discloses a birch sap anti-aging whitening composition, wherein although fermented birch sap is used as a cosmetic raw material, the fermentation strain, functional components and efficacy of the raw material are unknown, and there is a fermentation period of up to 30 days, production low efficiency
[0006] In addition, the existing process often uses organic solvents or concentrated treatment for the fermentation filtrate of Inonotus obliquus
For example, CN104956925A discloses the production method of continuous submerged fermentation broth and fermentation powder of Inonotus obliquus, wherein the fermentation filtrate is concentrated and processed, which has a higher process cost; CN106434755A discloses Inonotus obliquus submerged fermentation products and uses thereof, wherein Ethanol is used to elute the fermentation product, and the use of organic solvents is likely to cause environmental pollution and increase processing energy consumption

Method used

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  • Fermented birch juice and production method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1 : Preparation of fermented birch sap filtrate

[0063] (1) Preparation of seed solution

[0064] Scrape Inonotus obliquus from the solid PDA medium plate, inoculate it into a shaker flask containing 50mL of seed medium, culture at 28°C, shaker speed 150rpm, and cultivate for 4 days to obtain a first-class seed liquid; Under the condition, according to the inoculum amount of 10%, transfer the primary seed solution to the shake flask equipped with 400mL seed medium, and under the condition of 28 ℃ and 150rpm, keep the constant temperature for 2 days to obtain the secondary seed solution; The formula of the medium includes 1000mL deionized water, 3% glucose, 1% oat germ bran powder, 1% yeast powder, 0.1% potassium dihydrogen phosphate and 0.05% anhydrous magnesium sulfate, natural pH;

[0065] (2) Preparation of birch sap medium

[0066] The birch sap stock solution (brix 0.875) collected in the Daxinganling Mountains in Northeast China was used as a substrate...

Embodiment 2

[0072] Example 2 : Preparation of fermented birch sap filtrate

[0073] (1) Preparation of seed solution

[0074] Seed liquid preparation process is identical with embodiment 1;

[0075] (2) Preparation of birch sap medium

[0076] The birch sap stock solution (brix 1.3) collected in Finland was used as a substrate, 1.5% glucose was added as a carbon source, 1% pea flour (purchased from Cargill Grain and Oil (Nantong) Co., Ltd.) was added as a nitrogen source, and 0.1% phosphoric acid was added Potassium dihydrogen and 0.05% anhydrous magnesium sulfate, using 1M sodium citrate aqueous solution to adjust the pH of the birch sap medium to 5.8;

[0077] (3) Inoculation fermentation

[0078] According to the volume of 65% (v / v) liquid content, the prepared birch sap medium in step (2) is added in a 100L fermenter, and sterilized at 121°C for 30 minutes; 7% inoculum amount, put the secondary seed liquid into the fermenter under aseptic conditions, and ferment continuously for...

Embodiment 3

[0082] Example 3 : Preparation of fermented birch sap filtrate

[0083] (1) Preparation of seed solution

[0084] The preparation method of seed liquid is identical with embodiment 1;

[0085] (2) Preparation of birch sap medium

[0086] The birch sap concentrate (concentrated 3 times, brix 3.58) from the Northeast Xiaoxing’an Mountains was used as a substrate, 0.5% glucose was added as a carbon source, and 3% hydrolyzed oat protein (purchased from Xiamen Granbell Biotechnology Co., Ltd.) As a nitrogen source, add 0.1% potassium dihydrogen phosphate and 0.05% anhydrous magnesium sulfate, and use 1M sodium citrate aqueous solution to adjust the pH of the birch sap medium to 6.0;

[0087] (3) Inoculation fermentation

[0088] According to the volume of 65% (v / v) liquid, add the birch sap medium prepared in step (2) into a 500L fermenter, and sterilize at 121°C for 30 minutes; The inoculum amount of the secondary seed solution was inserted into the fermenter under aseptic c...

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Abstract

The invention relates to fermented birch juice and a production method thereof. The fermented birch juice is obtained through fermentation by taking inonotus obliquus as a strain and birch juice as asubstrate. The method comprises the steps of performing fermentation by taking the inonotus obliquus as a strain and the birch juice as a substrate.

Description

technical field [0001] The present invention relates to fermented birch sap and a production method thereof, wherein the fermented birch sap is obtained by fermentation using Inonotus obliquus as a strain and using birch sap as a substrate; Bacteria as a strain, the step of fermenting with birch sap as a substrate. Background technique [0002] Birch is a deciduous tree of the Betaceae family. There are currently about 100 species in the world, mainly distributed in the northern temperate zone and the cold temperate zone. Among them, there are about 29 varieties in my country, mainly distributed in the northeast, northwest, north and southwest of China. Most birch trees grow in remote mountainous areas with less human intervention and no industrial pollution. Birch sap (also known as birch sap) is the fresh sap from birch bark cut or trunk drilled. It is colorless or light yellow, free of sediment and impurities, and has a faint birch fragrance. Birch sap contains a lot o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/02C12P19/04C12P7/22A61K8/9789A61Q19/00A61Q19/02A61Q19/08C12R1/645
CPCC12P1/02C12P19/04C12P7/22A61K8/9789A61Q19/00A61Q19/08A61Q19/02A61K2800/85C12R2001/645C12N1/145A61K8/347A61K36/185A61K36/076A61K2236/19A61K2300/00A61K8/73A61K8/9728
Inventor 段颖异王昕悦洪涛
Owner NATURAL MEDICINE INST OF ZHEJIANG YANGSHENGTANG
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