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A fluorescent probe for identifying aluminum ions and its preparation method and application

A fluorescent probe and aluminum ion technology, applied in the field of fluorescent probes for aluminum ion identification, can solve the problems of difficulty in synthesis, interference, and high cost, and achieve the effects of simple preparation process, simple reaction conditions, and easy availability of raw materials

Active Publication Date: 2021-02-02
GUIZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a fluorescent probe for identifying aluminum ions and its preparation method and application for the above-mentioned deficiencies in the prior art, so as to solve the problem that the existing fluorescent probe proposed in the above-mentioned background technology is Some aluminum ion identification and detection are not specific enough, easily interfered by other ions, and some are costly, difficult to synthesize, and some membranes have poor permeability; some excitation wavelengths are in the ultraviolet region, which are applied to the detection of living organisms issues such as restrictions

Method used

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  • A fluorescent probe for identifying aluminum ions and its preparation method and application
  • A fluorescent probe for identifying aluminum ions and its preparation method and application
  • A fluorescent probe for identifying aluminum ions and its preparation method and application

Examples

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Embodiment 1

[0030] Example 1, the present invention provides a technical solution: a fluorescent probe for identifying aluminum ions, the molecular formula is C 24 h 25 N 2 o 3 , its structural formula:

[0031]

[0032] A method for preparing a fluorescent probe for identifying aluminum ions,

[0033]

[0034] Include the following steps,

[0035] S1. First add 1.14g (1R, 2R)-cyclohexanediamine and 60mL absolute ethanol to a 250mL three-necked bottle to obtain a (1R, 2R)-cyclohexanediamine solution, and simultaneously add 1.52g o-vanillin Dissolve in 50mL absolute ethanol solution to obtain o-vanillin solution, then add o-vanillin solution dropwise to (1R,2R)-cyclohexanediamine solution within half an hour, continue to stir, and react at a constant temperature of 50°C 30min;

[0036] S2. Add concentrated hydrochloric acid dropwise after the reaction is completed, the concentration of the concentrated hydrochloric acid is 1mol / L, adjust the pH of the solution to pH=4-5, and sp...

Embodiment 2

[0040] Example 2, verifying the selectivity of fluorescent probes to metal ions,

[0041] (1) Prepare fluorescent probe stock solution with a concentration of 4.0×10 -4 mol / L: Weigh 16.0mg of fluorescent probe, dissolve it in absolute ethanol, and prepare 100mL solution; Al 3+ stock solution 2.0×10 -3 mol / L: weigh 7.5mg Al(NO 3 ) 3 , dissolved in ethanol to prepare a 100mL solution; Al 3+ stock solution 4.0×10 -5 mol / L and 4.0×10 -4 mol / L by Al 3+ The stock solution was diluted to 2.0×10-3 mol / L, and all metal cations were prepared with ethanol to 2.0×10 -3 mol / L;

[0042] (2) Add fluorescent probe stock solution and metal cations in sequence to a 10.0mL volumetric flask, dilute to the mark with ethanol, shake well, place for 2 hours, transfer to a 1cm quartz cuvette for UV-visible absorption and fluorescence spectrum measurement, fluorescence The excitation and emission wavelengths of the spectroscopic determination are 364 / 480nm, and the ultraviolet-visible absorpti...

Embodiment 3

[0045] Embodiment 3, the fluorescence spectrum of fluorescent probe changes with aluminum ion concentration

[0046] The concentration of fixed fluorescent probe solution was 4.0×10 -5 mol / L, adding different concentrations of Al respectively 3+ , oscillated and shaken for 2 hours and then tested for fluorescence, the excitation wavelength was 346nm. The results are attached figure 2 As shown, the fluorescence spectrum of the fluorescent probe in ethanol solution varies with Al 3+ The change diagram of the concentration increase, the maximum emission wavelength of the fluorescent probe is at 480nm, with the increase of Al 3+ The concentration of the fluorescent probe increases continuously, but when Al 3+ After the concentration of the fluorescent probe is about twice that of the fluorescent probe, the fluorescence increase of the fluorescent probe becomes smaller.

[0047] In addition, in ethanol solution, the excitation and emission wavelengths are 364 / 480nm, and the a...

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Abstract

The invention provides a fluorescent probe for identifying aluminum ions and its preparation method and application. The molecular formula of the fluorescent probe is C 24 h 25 N 2 o 3 , the preparation method comprises the following steps: adding (1R, 2R)-cyclohexanediamine and dehydrated ethanol successively to a three-neck flask under N2 protection, and then adding o-vanillin to adjust the pH of the solution to pH=4~4 After 5, spin dry under vacuum to obtain a light yellow solid, and dissolve it in 100 mL of distilled water, wash with dichloromethane three times to obtain a yellow aqueous solution, slowly add ammonia water dropwise to pH = 9-10, wash with distilled water three times, and collect Dichloro, vacuum-dried with anhydrous sodium sulfate to obtain yellow semi-body compound A, then add compound A and anhydrous methanol to the three-necked flask in turn, then dissolve 2-hydroxy-1-naphthaldehyde in methanol solution and add to it , continue the reaction at room temperature and then spin dry until a solid is produced. The solid is washed with methanol three times to obtain a fluorescent probe. This method is simple, fast, easy to operate, and has good selectivity and high sensitivity.

Description

technical field [0001] The invention belongs to the technical field of fluorescent probes for identifying aluminum ions, and in particular relates to a fluorescent probe for identifying aluminum ions, a preparation method and application thereof. Background technique [0002] Aluminum is the metal element with the highest content in the earth's crust. With the extensive use of aluminum products and aluminum-containing compounds, the content of aluminum ions in water and soil increases significantly. Excessive intake of aluminum will affect the absorption of calcium in the human stomach, leading to rickets and organ atrophy. Moreover, it can also cause damage to the central nervous system of the human body, causing diseases such as Parkinson's disease and Alzheimer's disease. Therefore, the detection of aluminum ions in the environment is particularly important. The traditional methods for detecting aluminum ions include atomic absorption, atomic emission, electrochemical an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C251/24C07C249/02C09K11/06G01N21/64
CPCC07C249/02C07C251/24C09K11/06C09K2211/1007C07C2601/14G01N21/643
Inventor 张奇龙王家忠谢海芳徐红黄亚励
Owner GUIZHOU MEDICAL UNIV
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