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Fluorescent probe for identifying aluminum ions and preparation method and application of fluorescent probe

A technology of fluorescent probes and aluminum ions, which is applied in the field of fluorescent probes for aluminum ion identification, can solve the problems of difficult synthesis, interference, and high cost, and achieve the effects of simple preparation process, simple reaction conditions, and simple operation

Active Publication Date: 2020-02-07
GUIZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a fluorescent probe for identifying aluminum ions and its preparation method and application for the above-mentioned deficiencies in the prior art, so as to solve the problem that the existing fluorescent probe proposed in the above-mentioned background technology is Some aluminum ion identification and detection are not specific enough, easily interfered by other ions, and some are costly, difficult to synthesize, and some membranes have poor permeability; some excitation wavelengths are in the ultraviolet region, which are applied to the detection of living organisms issues such as restrictions

Method used

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  • Fluorescent probe for identifying aluminum ions and preparation method and application of fluorescent probe
  • Fluorescent probe for identifying aluminum ions and preparation method and application of fluorescent probe
  • Fluorescent probe for identifying aluminum ions and preparation method and application of fluorescent probe

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Embodiment 1

[0030] Example 1, the present invention provides a technical solution: a fluorescent probe for identifying aluminum ions, the molecular formula is C 24 h 25 N 2 o 3 , its structural formula:

[0031]

[0032] A method for preparing a fluorescent probe for identifying aluminum ions,

[0033]

[0034] Include the following steps,

[0035] S1. First add 1.14g (1R, 2R)-cyclohexanediamine and 60mL absolute ethanol to a 250mL three-necked bottle to obtain a (1R, 2R)-cyclohexanediamine solution, and simultaneously add 1.52g o-vanillin Dissolve in 50mL absolute ethanol solution to obtain o-vanillin solution, then add o-vanillin solution dropwise to (1R,2R)-cyclohexanediamine solution within half an hour, continue to stir, and react at a constant temperature of 50°C 30min;

[0036] S2. Add concentrated hydrochloric acid dropwise after the reaction is completed, the concentration of the concentrated hydrochloric acid is 1mol / L, adjust the pH of the solution to pH=4-5, and sp...

Embodiment 2

[0040] Example 2, verifying the selectivity of fluorescent probes to metal ions,

[0041] (1) Prepare fluorescent probe stock solution with a concentration of 4.0×10 -4 mol / L: Weigh 16.0mg of fluorescent probe, dissolve it in absolute ethanol, and prepare 100mL solution; Al 3+ stock solution 2.0×10 -3 mol / L: weigh 7.5mg Al(NO 3 ) 3 , dissolved in ethanol to prepare a 100mL solution; Al 3+ stock solution 4.0×10 -5 mol / L and 4.0×10 -4 mol / L by Al 3+ The stock solution was diluted to 2.0×10-3 mol / L, and all metal cations were prepared with ethanol to 2.0×10 -3 mol / L;

[0042] (2) Add fluorescent probe stock solution and metal cations in sequence to a 10.0mL volumetric flask, dilute to the mark with ethanol, shake well, place for 2 hours, transfer to a 1cm quartz cuvette for UV-visible absorption and fluorescence spectrum measurement, fluorescence The excitation and emission wavelengths of the spectroscopic determination are 364 / 480nm, and the ultraviolet-visible absorpti...

Embodiment 3

[0045] Embodiment 3, the fluorescence spectrum of fluorescent probe changes with aluminum ion concentration

[0046] The concentration of fixed fluorescent probe solution was 4.0×10 -5 mol / L, adding different concentrations of Al respectively 3+ , oscillated and shaken for 2 hours and then tested for fluorescence, the excitation wavelength was 346nm. The results are attached figure 2 As shown, the fluorescence spectrum of the fluorescent probe in ethanol solution varies with Al 3+ The change diagram of the concentration increase, the maximum emission wavelength of the fluorescent probe is at 480nm, with the increase of Al 3+ The concentration of the fluorescent probe increases continuously, but when Al 3+ After the concentration of the fluorescent probe is about twice that of the fluorescent probe, the fluorescence increase of the fluorescent probe becomes smaller.

[0047] In addition, in ethanol solution, the excitation and emission wavelengths are 364 / 480nm, and the a...

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Abstract

The invention provides a fluorescence probe for identifying aluminum ions and a preparation method and application of the fluorescent probe. The molecular formula of the fluorescence probe is C24H25N2O3. The preparation method comprises the following steps: in the presence of N2, sequentially adding (1R,2R)-hexamethylene diamine and anhydrous ethanol into a three-neck bottle, further adding o-vanillin, adjusting the pH value of the solution to pH=4-5, and performing vacuum spinning drying so as to obtain a light yellow solid; dissolving the light yellow solid into 100mL of distilled water, andperforming washing for three times by using dichloromethane so as to obtain a yellow aqueous liquid; slowly dropwise adding ammonium hydroxide till pH=9-10, performing washing for three times by using distilled water, collecting dichloride, and performing vacuum drying by using anhydrous sodium sulfate so as to obtain a yellow half body compound A; and further sequentially adding the compound A and anhydrous methanol into the three-neck bottle, dissolving 2-hydroxyl-1-naphthalic aldehyde into the methanol solution, adding the solution into the three-neck bottle, performing a reaction continuously at room temperature till a solid is generated, and washing the solid for three times by using a methanol lotion, so as to obtain the fluorescent probe. The method is simple and rapid, easy to operate, good in selectivity and high in sensitivity.

Description

technical field [0001] The invention belongs to the technical field of fluorescent probes for identifying aluminum ions, and in particular relates to a fluorescent probe for identifying aluminum ions, a preparation method and application thereof. Background technique [0002] Aluminum is the metal element with the highest content in the earth's crust. With the extensive use of aluminum products and aluminum-containing compounds, the content of aluminum ions in water and soil increases significantly. Excessive intake of aluminum will affect the absorption of calcium in the human stomach, leading to rickets and organ atrophy. Moreover, it can also cause damage to the central nervous system of the human body, causing diseases such as Parkinson's disease and Alzheimer's disease. Therefore, the detection of aluminum ions in the environment is particularly important. The traditional methods for detecting aluminum ions include atomic absorption, atomic emission, electrochemical an...

Claims

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Application Information

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IPC IPC(8): C07C251/24C07C249/02C09K11/06G01N21/64
CPCC07C249/02C07C251/24C09K11/06C09K2211/1007C07C2601/14G01N21/643
Inventor 张奇龙王家忠谢海芳徐红黄亚励
Owner GUIZHOU MEDICAL UNIV
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